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A Novel Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Promoter for Expressing Transgenes in the Halotolerant Alga Dunaliella salina

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Abstract

A major challenge for efficient transgene expression in Dunaliella salina is to find strong endogenous promoters to drive the transgene expression. In the present study, a novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter was cloned and used to drive expressions of the bialaphos resistance (bar) gene and of the N-terminal fragment of human canstatin (Can-N). The results showed that the bar gene was transcribed by the GAPDH promoter and integrated into the genome of the transformants of D. salina. Furthermore, the PCR identification, Southern and western blots indicated that Can-N was expressed in transgenic D. salina, demonstrating that the promoter of the D. salina GAPDH gene is suitable for driving expression of heterologous genes in transgenic D. salina.

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Acknowledgments

This study was supported by the grant from International Science and Technology Cooperation Program of the Ministry of Science and Technology of PR China (No. 2007DFA01240).

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Authors and Affiliations

  1. Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, Henan, China

    Yanlong Jia, Shenke Li, Shuying Feng & Lexun Xue

  2. Pharmacy College, Xinxiang Medical University, Xinxiang, 453003, Henan, China

    Yanlong Jia

  3. Puyang City Oil Field General Hospital, Puyang, 457001, Henan, China

    Shenke Li

  4. Department of Horticultural Science, North Carolina State University, Raleigh, NC, 27606, USA

    George Allen

  5. Institute of Biomedical Engineering, Henan University of Science and Technology, Luoyang, 471003, Henan, China

    Shuying Feng

  6. Laboratory for Cell Biology, Department of Biology, Zhengzhou University, Zhengzhou, 450001, Henan, China

    Lexun Xue

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  1. Yanlong Jia
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  2. Shenke Li
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  3. George Allen
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  4. Shuying Feng
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  5. Lexun Xue
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Correspondence to Lexun Xue.

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Jia, Y., Li, S., Allen, G. et al. A Novel Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Promoter for Expressing Transgenes in the Halotolerant Alga Dunaliella salina . Curr Microbiol 64, 506–513 (2012). https://doi.org/10.1007/s00284-012-0102-y

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  • DOI: https://doi.org/10.1007/s00284-012-0102-y

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