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Towards the development of a nuclear transformation system for Dunaliella tertiolecta

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Abstract

A nuclear transformation system for the microalga Dunaliella tertiolecta was explored using electroporation. Plasmids incorporating the D. tertiolecta RbcS1 5′ and 3′ untranslated regions flanking the Streptoalloteichus hindustanus gene encoding bleomycin resistance (ble) were introduced into D. tertiolecta cells both transiently and stably. Southern hybridisation was used to examine the fate of the plasmid following electroporation and revealed that the DNA was entering the cells but was quickly degraded. Using the same procedure one stably transformed line was recovered.

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Abbreviations

ble:

bleomycin resistance gene

RbcS:

ribulose bisphosphate carboxylase/oxygenase small subunit

References

  • Ben-Amotz A, Avron M (1990) The biotechnology of cultivating the halotolerant alga Dunaliella. Trends Biotechnol. 8: 121–126.

    Article  Google Scholar 

  • Brown LE, Sprecher SL, Keller LR (1991) Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation. Mol. Cell Biol. 11: 2328–2332.

    PubMed  Google Scholar 

  • Chen Y, Wang Y, Sun Y, Zhang L, Li W (2001) Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells. Curr. Genet. 39: 365–370.

    Article  PubMed  Google Scholar 

  • Geng D, Wang Y, Wang P, Li W, Sun Y (2003) Stable expression of hepatitis B surface antigen in Dunaliella salina (Chlorophyta). J. Appl. Phycol. 15: 451–456.

    Article  Google Scholar 

  • Jin ES, Polle JE, Melis A (2001) Involvement of zeaxanthin and of the Cbr protein in the repair of photosystem II from photoinhibition in the green alga Dunaliella salina. Biochim. Biophys. Acta 1506: 244–259.

    PubMed  Google Scholar 

  • Lumbreras V, Stevens DR, Purton S (1998) Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron. Plant J. 14: 441–447.

    Article  Google Scholar 

  • Peto R, Doll R, Buchley JD, Sporn MB (1981) Can dietary beta-carotene materially reduce human cancer rates? Nature (Lond) 290-201–207.

    Article  Google Scholar 

  • Porath JB, Gokhman I, Lers A, Levy A, Zamir A (1997) Developing a transformation system in Dunaliella. Phycologia 36(Suppl): 89.

    Google Scholar 

  • Provasoli L, McLaughlin JAA, Droop MR (1957) The development of artificial media for marine microalgae. Archiv fur Mikrobiologie 25: 392–428.

    Article  PubMed  Google Scholar 

  • Stevens DR, Rochaix JD, Purton S (1996) The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas. Mol. Gen. Genet. 251: 23–30.

    Article  PubMed  Google Scholar 

  • Suda D, Schwartz J, Shklar G (1986) Inhibition of experimental oral carcinogenesis by topical beta-carotene. Carcinogenesis 7: 711–715.

    PubMed  Google Scholar 

  • Walker TW, Becker DK, Collet C (2005) Characterisation of the Dunaliella tertiolecta RbcS genes and their promoter activity in Chlamydomonas reinhardtii. Plant Cell Rep. 23: 727–735.

    Article  PubMed  Google Scholar 

  • Widholm JMC (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47: 189–194.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Cluster for Molecular Biotechnology, Science Research Centre and CRC for Diagnostics, Queensland University of Technology, GPO Box 2434, Brisbane, QLD, 4001, Australia

    Tara L. Walker, Douglas K. Becker, James L. Dale & Chris Collet

Authors
  1. Tara L. Walker
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  2. Douglas K. Becker
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  3. James L. Dale
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  4. Chris Collet
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Correspondence to Chris Collet.

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Walker, T.L., Becker, D.K., Dale, J.L. et al. Towards the development of a nuclear transformation system for Dunaliella tertiolecta. J Appl Phycol 17, 363–368 (2005). https://doi.org/10.1007/s10811-005-4783-5

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  • DOI: https://doi.org/10.1007/s10811-005-4783-5

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