Skip to main content
SpringerLink
Log in

Characterization of Adhesive Exopolysaccharide (EPS) Produced by Pseudomonas aeruginosa Under Starvation Conditions

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Pseudomonas aeruginosa synthesizes large quantities of exopolysaccharide (EPS), making it an excellent model organism for the study of EPS-mediated adhesion. The purpose of this investigation was to evaluate the influence of limited nutrients availability in the culture medium on the composition of EPS produced by P. aeruginosa. The relationship between the EPS production and the adhesion process of the P. aeruginosa cells to stainless steel surface (type 316 L) under starvation conditions were also examined. In all experimental variants P. aeruginosa produced more EPS with an increase of incubation period upon starvation conditions. Under limited nutrients condition, glucose dominated in the EPS materials. After 6 days of the process, only glucosyl units were detected in the extracellular matrix produced by nutrient-deprived P. aeruginosa cells. These extracellular molecules promoted more advanced stages of P. aeruginosa biofilm formation on the surface of stainless steel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Allison DA, Sutherland IW (1987) The role of exopolysaccharides in adhesion of freshwater bacteria. J Gen Microbiol 133:1319–1327

    CAS  Google Scholar 

  2. Bower CK, McGuire J, Daeschel MA (1996) The adhesion and detachment of bacteria and spores on food-contact surfaces. Trends Food Sci Tech 7:152–157

    Article  CAS  Google Scholar 

  3. Celik GY, Aslim B, Beyatli Y (2008) Characterization and production of the exopolysaccharide (EPS) from Pseudomonas aeruginosa G1 and Pseudomonas putida G12 strains. Carbohydr Polym 73:178–182

    Article  CAS  Google Scholar 

  4. Chen X, Stewart PS (2002) Role of electrostatic interactions in cohesion of bacterial biofilms. Appl Microbiol Biotechnol 59:718–722

    Article  PubMed  CAS  Google Scholar 

  5. Costerton JW, Cheng K-I, Geesey G (1981) Bacterial biofilms in nature and disease. Annu Rev Microbiol 35:399–424

    Article  Google Scholar 

  6. Drenkard E (2003) Antimicrobial resistance of Pserudomonas aeruginosa biofilms. Microbes Infect 5:1213–1219

    Article  PubMed  CAS  Google Scholar 

  7. Dunne WM (2002) Bacterial adhesion: seen any good biofilms lately? Clin Microbiol Rev 15:155–166

    Article  PubMed  CAS  Google Scholar 

  8. Forde A, Fitzgerald GF (1999) Analysis of exopolysaccharide (EPS) production mediated by the bacteriphage adsorption blocking plasmid, pCI658, isolated from Lactococcus lactis ssp. cremoris HO2. Int Dairy J 9:465–472

    Article  CAS  Google Scholar 

  9. Friedman L, Kolter R (2004) Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms. Mol Microbiol 51:675–690

    Article  PubMed  CAS  Google Scholar 

  10. Fuster-Valls N, Hernandez-Herrero M, Marin-de-Mateo M et al (2008) Effect of different environmental conditions on the bacteria survival on stainless steel surfaces. Food Control 19:308–314

    Article  CAS  Google Scholar 

  11. Gram L, Ravn L, Rasch M et al (2002) Food spoilage––interactions between food spoilage bacteria. Int J Food Microbiol 78:79–97

    Article  PubMed  Google Scholar 

  12. Grobe S, Wingender J, Flemming H-C (2001) Capability of mucoid Pseudomonas aeruginosa to survive in chlorinated water. Int J Hyg Environ Health 204:139–142

    Article  PubMed  CAS  Google Scholar 

  13. Hung C-C, Santschi PH, Gillow JB (2005) Isolation and characterization of extracellular polysaccharides produced by Pseudomonas fluorescens Biovar II. Carbohydr Polym 61:141–147

    Article  CAS  Google Scholar 

  14. Kiliς NK, Dönmez G (2008) Environmental conditions affecting exopolysaccharide production by Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp. J Hazard Mater 154:1019–1024

    Article  Google Scholar 

  15. Le Thi TT, Prigent-Combaret C, Dorel C et al (2001) First stages of biofilm formation: characterization and quantification of bacterial functions involved in colonization process. Methods Enzymol 336:152–159

    Article  PubMed  CAS  Google Scholar 

  16. Parkar SG, Flint SH, Palmer JS et al (2001) Factors influencing attachment of thermophilic bacilli to stainless steel. J Appl Microbiol 11:675–685

    Google Scholar 

  17. Pontefract RD (1991) Bacterial adherence: its consequences in food processing. Can I Food Sci Tech J 24:113–117

    Google Scholar 

  18. Precival SL, Knapp JS, Edyvean RGJ et al (1998) Biofilms, mains water and stainless steel. Water Res 32:2187–2201

    Article  Google Scholar 

  19. Sanin SL, Sanin SD, Bryers JD (2003) Effect of starvation on the adhesive properties of xenobiotic degrading bacteria. Process Biochem 38:909–914

    Article  CAS  Google Scholar 

  20. Schubert R (1989) The use of arginine briliantgreen glucose peptone broth (ABGP medium) as a primary culture medium for Pseudomonas aeruginosa. Zbl Bakt Mik Hyg 187:166–268

    Google Scholar 

  21. Wai SN, Mizunoe Y, Yoshida S (1999) How Vibrio cholerae survive during starvation. FEMS Microbiol Lett 180:123–131

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgment

This study was supported by the Ministry of Science and Higher Education, Poland, project no NN 312326533.

Author information

Authors and Affiliations

  1. Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, Poznan, 60-627, Poland

    Kamila Myszka & Katarzyna Czaczyk

Authors
  1. Kamila Myszka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katarzyna Czaczyk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kamila Myszka.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Myszka, K., Czaczyk, K. Characterization of Adhesive Exopolysaccharide (EPS) Produced by Pseudomonas aeruginosa Under Starvation Conditions. Curr Microbiol 58, 541–546 (2009). https://doi.org/10.1007/s00284-009-9365-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-009-9365-3

Keywords

Search

Navigation