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Effect of Radiofrequency Ablation of the Liver on Cell-Mediated Immunity in Rats

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Abstract

We investigated the effect of radiofrequency ablation (RFA) of the liver on cell-mediated immunity in rats. Sprague-Dawley rats were divided into five groups: control group that did not have any procedure, a sham-operation group that underwent laparotomy, a lobectomy group that had left lateral lobectomy of the liver, a one-lobe RFA group that had RFA on the left lateral lobe of the liver, and a two-lobe RFA group that had RFA on the left lateral and left median lobe of the liver. Delayed-type hypersensitivity (DTH) was induced by challenging the ears of previously sensitized rats with 2,4-dinitro-1-fluorobenzene. Cell-mediated immunity was evaluated by direct measurement of the ear thickness and quantitative analysis of leukocytic infiltration of the tissue. The increased ear thickness was 42.0%, 69.2%, 61.8%, 46.7%, and 39.8% from the baseline one day after each procedure, and the mean leukocytic infiltration into the ear tissue was 3136.7, 3895.0, 3913.3, 2803.3, and 2316.7/mm2 seven days after each procedure in the control, sham-operation, lobectomy, one-lobe RFA, and two-lobe RFA groups, respectively. In conclusion, RFA of the liver tissue can partially abolish the augmentation of DTH and leukocytic infiltration those were seen in the hepatic lobectomy and sham-operation groups. The procedure had a negative effect on cell-mediated immunity in rats, and the intensity of RFA showed a reverse correlation with cell-mediated immunity.

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Acknowledgments

This study was supported in part by clinical research fund Research Institute of Clinical Medicine, Chonbuk National University Hospital.

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Authors and Affiliations

  1. Department of Surgery, Chonbuk National University Medical School, 634-18 Keumam-dong, Dukjin-gu, Jeonju, Jeonbuk, 561-180, Korea

    Hee Chul Yu M.D., PhD., Zhe-Wu Jin M.D., Dae Yung Lee M.D., Chan Young Kim M.D. & Baik Hwan Cho M.D., Ph.D.

  2. Division of Liver/GI Transplant, Department of Surgery, University of Miami School of Medicine, 1801 NW 9th Ave., Suite 507, Miami, Florida 33136

    Jang Il Moon M.D.

  3. Department of Anatomy, Chonbuk National University Medical School, 634–18 Keumam-dong, Dukjin-gu Jeonju, Jeonbuk, 561–712, Korea

    Chang Ho Song M.D., PhD.

  4. Research Institute of Clinical Medicine, Chonbuk National University Hospital, 634–18 Keumam-dong, Dukjin-gu Jeonju, Jeonbuk, 561–712, Korea

    Hee Chul Yu M.D., PhD. & Baik Hwan Cho M.D., Ph.D.

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  1. Hee Chul Yu M.D., PhD.
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  2. Jang Il Moon M.D.
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  3. Zhe-Wu Jin M.D.
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  4. Dae Yung Lee M.D.
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  6. Chang Ho Song M.D., PhD.
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Correspondence to Baik Hwan Cho M.D., Ph.D..

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Yu, H.C., Il Moon, J., Jin, ZW. et al. Effect of Radiofrequency Ablation of the Liver on Cell-Mediated Immunity in Rats . World J. Surg. 29, 739–743 (2005). https://doi.org/10.1007/s00268-005-7728-x

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  • DOI: https://doi.org/10.1007/s00268-005-7728-x

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