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Smoking and Physical Activity Significantly Influence Stromal Vascular Fraction Cell Yield and Viability

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  • Basic Science/Experimental
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Abstract

Background

Subcutaneous tissue is an abundant source of adipose-derived regenerative cells. It is readily available and easy to extract by means of liposuction, making it one of the most popular sources for tissue engineering and regenerative medical applications.

Methods

The stromal vascular fraction (SVF) cell yield and viability of the lipoaspirate obtained from 43 patients undergoing elective liposuction were examined in correlation with their age, gender, body mass index, smoking status, and physical activity. The lipoaspirate was processed with the Celution® 800/CRS system to isolate the SVF and a few drops of the obtained pellet were used for cell counting with NecleoCounter® NC-100TM.

Results

Twenty-eight (65.1%) were men and 15 (34.9%) were women with an average age of 40.7 ± 10.4 years (women) and 38.9 ± 11.8 years (men). Viable SVF cells/g fat was significantly correlated with smoking level (negative correlation, ρ=  − 0.312, P < 0.05) and with marginal significance with female gender. Cell viability showed a significant negative correlation with physical activity level (ρ =   − 0.432, P < 0.01); borderline significance for correlation of this parameter with smoking level should not be neglected. Other parameters did not influence the cell yield nor the viability of the stromal vascular fraction.

Conclusion

Many factors may influence SVF cell yield and viability. Our findings indicate that age and smoking significantly influenced SVF cell yield, age positively while smoking negatively. Increased physical activity had a negative correlation with SVF cell viability.

Level of Evidence N/A

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Authors and Affiliations

  1. Faculty of Medicine, University of Belgrade, BelPrime Clinic, 16 Brane Crncevica, 11000, Belgrade, Serbia

    Katarina Andjelkov

  2. Boca Raton, Florida, USA

    Alexandra Conde-Green

  3. University College of London, London, UK

    Afshin Mosahebi

Authors
  1. Katarina Andjelkov
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  2. Alexandra Conde-Green
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  3. Afshin Mosahebi
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Correspondence to Katarina Andjelkov.

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The authors declare that they have no conflict of interest.

Ethical Standard

The study was approved by the ethics committee of the First Surgical Clinic, faculty of medicine, University of Belgrade, Serbia and every patient gave written consent prior to the procedure.

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Appendix

Appendix

See Table 4.

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Andjelkov, K., Conde-Green, A. & Mosahebi, A. Smoking and Physical Activity Significantly Influence Stromal Vascular Fraction Cell Yield and Viability. Aesth Plast Surg 45, 315–321 (2021). https://doi.org/10.1007/s00266-020-02008-2

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  • DOI: https://doi.org/10.1007/s00266-020-02008-2

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