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The effect of reactive oxygen species (ROS) and ROS-scavenging enzymes, superoxide dismutase and catalase, on the thermotolerant ability of Corynebacterium glutamicum

  • Applied microbial and cell physiology
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Abstract

The function of two reactive oxygen species (ROS) scavenging enzymes, superoxide dismutase (SOD) and catalase, on the thermotolerant ability of Corynebacterium glutamicum was investigated. In this study, the elevation of the growth temperature was shown to lead an increased intracellular ROS for two strains of Corynebacterium glutamicum, the wild-type (KY9002) and the temperature-sensitive mutant (KY9714). In order to examine the effects of ROS-scavenging enzymes on cell growth, either the SOD or the catalase gene was disrupted or overexpressed in KY9002 and KY9714. In the case of the KY9714 strain, it was shown that the disruption of SOD and catalase disturbs cell growth, while the over-productions of both the enzymes enhances cell growth with a growth temperature of 30 °C and 33 °C. Whereas, in the relatively thermotolerant KY9002 strain, the disruption of both enzymes exhibited growth defects more intensively at higher growth temperatures (37 °C or 39 °C), while the overexpression of at least SOD enhanced the cell growth at higher temperatures. Based on the correlation between the cell growth and ROS level, it was suggested that impairment of cell growth in SOD or catalase-disrupted strains could be a result of an increased ROS level. In contrast, the improvement in cell growth for strains with overexpressed SOD or catalase resulted from a decrease in the ROS level, especially at higher growth temperatures. Thus, SOD and catalase might play a crucial role in the thermotolerant ability of C. glutamicum by reducing ROS-induced temperature stress from higher growth temperatures.

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Acknowledgements

The authors wish to thank Sayoko Tanigawa and Seisuke Hatakeyama for their technical support. Part of this work was performed through collaboration with the Core to Core Program, which was supported by the Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT).

Funding

This work was supported financially by the Advanced Low Carbon Technology Research and Development Program (ALCA: JPMJAL1106) of Japan Science and Technology Agency (JST).

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Authors and Affiliations

  1. School of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 3000, Thailand

    Nawarat Nantapong

  2. Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515, Japan

    Ryutarou Murata, Toshiharu Yakushi & Kazunobu Matsushita

  3. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Sarvitr Trakulnaleamsai

  4. Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi, 753-8515, Japan

    Naoya Kataoka, Toshiharu Yakushi & Kazunobu Matsushita

  5. Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yamaguchi, 753-8515, Japan

    Naoya Kataoka, Toshiharu Yakushi & Kazunobu Matsushita

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  1. Nawarat Nantapong
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Correspondence to Nawarat Nantapong.

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Nantapong, N., Murata, R., Trakulnaleamsai, S. et al. The effect of reactive oxygen species (ROS) and ROS-scavenging enzymes, superoxide dismutase and catalase, on the thermotolerant ability of Corynebacterium glutamicum. Appl Microbiol Biotechnol 103, 5355–5366 (2019). https://doi.org/10.1007/s00253-019-09848-2

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  • DOI: https://doi.org/10.1007/s00253-019-09848-2

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