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Production of l-valine from metabolically engineered Corynebacterium glutamicum

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Abstract

l-Valine is one of the three branched-chain amino acids (valine, leucine, and isoleucine) essential for animal health and important in metabolism; therefore, it is widely added in the products of food, medicine, and feed. l-Valine is predominantly produced through microbial fermentation, and the production efficiency largely depends on the quality of microorganisms. In recent years, continuing efforts have been made in revealing the mechanisms and regulation of l-valine biosynthesis in Corynebacterium glutamicum, the most utilitarian bacterium for amino acid production. Metabolic engineering based on the metabolic biosynthesis and regulation of l-valine provides an effective alternative to the traditional breeding for strain development. Industrially competitive l-valine-producing C. glutamicum strains have been constructed by genetically defined metabolic engineering. This article reviews the global metabolic and regulatory networks responsible for l-valine biosynthesis, the molecular mechanisms of regulation, and the strategies employed in C. glutamicum strain engineering.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31370131), Post-graduate Research & Practice Innovation Program of Jiangsu Province (CXZZ12-0755), and the Collaborative Innovation Center of Jiangsu Modern Industrial Fermentation.

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  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Xiaoyuan Wang

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Xiaoyuan Wang & Hailing Zhang

  3. Department of Biochemistry, King’s College London, 150 Stamford Street, London, SE1 9NH, UK

    Peter J. Quinn

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Wang, X., Zhang, H. & Quinn, P.J. Production of l-valine from metabolically engineered Corynebacterium glutamicum. Appl Microbiol Biotechnol 102, 4319–4330 (2018). https://doi.org/10.1007/s00253-018-8952-2

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