Abstract
Brevibacterium flavum is an important microorganism for the production of amino acids in industrial fermentation. Knowledge of promoters in B. flavum is essential for efficient modulation of gene expression in metabolic engineering. Here we have constructed a novel E. coli-B. flavum promoter-probe vector pDXW-11. The pDXW-11 habors an oriE for replication in E. coli, genes dso and sso for replication in B. flavum, a kan gene used as selected marker, a multiple cloning sites preceded by a rrnBT1T2 terminator and sequentially followed by stop codons, an SD sequence and a cat reporter gene. Using pDXW-11, activities of several promoters were evaluated in B. flavum. A strong promoter, the tac-M promoter, was designed. The tac-M promoter would be very useful for metabolic engineering research in B. flavum.
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Acknowledgments
This work was supported by Chinese 863 National High-Tech Research and Development Plan Project (No. 2007AA02Z229 and No. 2007AA02Z230), the 111 Project (No. 111-2-06) and the Basic Research Programs of Jiangsu Province (BK2009003).
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Xu, D., Tan, Y., Li, Y. et al. Construction of a novel promoter-probe vector and its application for screening strong promoter for Brevibacterium flavum metabolic engineering. World J Microbiol Biotechnol 27, 961–968 (2011). https://doi.org/10.1007/s11274-010-0539-8
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DOI: https://doi.org/10.1007/s11274-010-0539-8