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Innate immune response, intestinal morphology and microbiota changes in Senegalese sole fed plant protein diets with probiotics or autolysed yeast

  • Applied microbial and cell physiology
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Abstract

The effects of using plant ingredients in Senegalese sole (Solea senegalensis) diet on immune competence and intestine morphology and microbial ecology are still controversial. Probiotics or immunostimulants can potentially alter the intestinal microbiota in a way that protects fish against pathogens. The current study aimed to examine the intestine histology and microbiota and humoral innate immune response in juvenile sole fed diets with low (35 %) or high (72 %) content of plant protein (PP) ingredients supplemented with a multispecies probiotic bacteria or autolysed yeast. Fish fed the probiotic diet had lower growth performance. Lysozyme and complement activities were significantly higher in fish fed PP72 diets than in their counterparts fed PP35 diets after 17 and 38 days of feeding. At 2 days of feeding, fish fed unsupplemented PP72 showed larger intestine section area and longer villus than fish fed unsupplemented PP35. At 17 days of feeding, fish fed unsupplemented PP72 showed more goblet cells than the other dietary groups, except the group fed yeast supplemented PP35 diet. High dietary PP level, acutely stimulate fish innate immune defence of the fish after 2 and 17 days of feeding. However, this effect does not occur after 73 days of feeding, suggesting a habituation to dietary treatments and/or immunosuppression, with a reduction in the number of the goblet cells. Fish fed for 38 days with diets supplemented with autolysed yeast showed longer intestinal villus. The predominant bacteria found in sole intestine were Vibrio sp. and dietary probiotic supplementation caused a reduction in Vibrio content, regardless of the PP level.

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Acknowledgments

We would like to thank to CIIMAR/ICBAS (UP) and FBA (University of Nordland) for the use of the facilities and equipment and for technical support and Mrs. Ligia Lourenço (UTAD) for her histology technical assistance.

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Authors and Affiliations

  1. ICBAS – Instituto de Ciências Biomédicas de Abel Salazar, Universidade de Porto, Rua Jorge Viterbo Ferreira n.° 228, 4050-313, Porto, Portugal

    S. Batista, L. M. P. Valente & R. O. A. Ozório

  2. CIMAR/CIIMAR – Centro Interdisciplinar de Investigação Marinha e Ambiental, Rua dos Bragas, 289, 4050-123, Porto, Portugal

    S. Batista, K. Sansuwan, L. M. P. Valente & R. O. A. Ozório

  3. FBA - Faculty of Biosciences and Aquaculture, Nord University, 8049, Bodø, Norway

    S. Batista & J. M. O. Fernandes

  4. Department of Microbiology, Faculty of Sciences, University of Málaga, 29071, Málaga, Spain

    A. Medina & M. A. Moriñigo

  5. CECAV – Centro de Ciência Animal e Veterinária, UTAD, Apartado 1013, 5000-801, Vila Real, Portugal

    M. A. Pires

  6. Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand

    K. Sansuwan

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  1. S. Batista
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  7. L. M. P. Valente
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  8. R. O. A. Ozório
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Correspondence to R. O. A. Ozório.

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Funding

S. M. G. Batista was supported by FCT – SFRH/BD/76668/2011. This work was also supported by the FCT Projects PEst-OE/AGR/UI0772/2011 and PEst-OE/AGR/UI0772/2014.

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All authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Batista, S., Medina, A., Pires, M.A. et al. Innate immune response, intestinal morphology and microbiota changes in Senegalese sole fed plant protein diets with probiotics or autolysed yeast. Appl Microbiol Biotechnol 100, 7223–7238 (2016). https://doi.org/10.1007/s00253-016-7592-7

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  • DOI: https://doi.org/10.1007/s00253-016-7592-7

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