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Peculiarities and applications of galactanolytic enzymes that act on type I and II arabinogalactans

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Abstract

Arabinogalactans (AGs) are branched galactans to which arabinose residues are bound as side chains and are widely distributed in plant cell walls. They can be grouped into two types based on the structures of their backbones. Type I AGs have β-1,4-galactan backbones and are often covalently linked to the rhamnogalacturonan-I region of pectins. Type II AGs have β-1,3-galactan backbones and are often covalently linked to proteins. The main enzymes involved in the degradation of AGs are endo-β-galactanases, exo-β-galactanases, and β-galactosidases, although other enzymes such as α-l-arabinofuranosidases, β-l-arabinopyranosidases, and β-d-glucuronidases are required to remove the side chains for efficient degradation of the polysaccharides. Galactanolytic enzymes have a wide variety of potential uses, including the bioconversion of AGs to fermentable sugars for production of commodity chemicals like ethanol, biobleaching of cellulose pulp, modulation of pectin properties, improving animal feed, and determining the chemical structure of AGs. This review summarizes our current knowledge about the biochemical properties and potential applications of AG-degrading enzymes.

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References

  • Ahn YO, Zheng M, Bevan DR, Esen A, Shiu SH, Benson J, Peng HP, Miller JT, Cheng CL, Poulton JE, Shih MC (2007) Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35. Phytochemistry 68:1510–1520

    Article  CAS  Google Scholar 

  • Bonnin E, Lahaye M, Vigouroux J, Thibault JF (1995) Preliminary characterization of a new exo-β-(1,4)-galactanase with transferase activity. Int J Biol Macromol 17:345–351

    Article  CAS  Google Scholar 

  • Bonnin E, Thibault JF (1996) Galactooligosaccharide production by transfer reaction of an exogalactanase. Enzyme Microb Technol 19:99–106

    Article  CAS  Google Scholar 

  • Braithwaite KL, Barna T, Spurway TD, Charnock SJ, Black GW, Hughes N, Lakey JH, Virden R, Hazlewood GP, Henrissat B, Gilbert HJ (1997) Evidence that galactanase a from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues. Biochem 36:15489–15500

    Article  CAS  Google Scholar 

  • Brillouet JM, Williams P, Moutounet M (1991) Purification and some properties of a novel endo-β-(1→6)-d-galactanase from Aspergillus niger. Agric Biol Chem 55:1565–1571

    Article  CAS  Google Scholar 

  • Bro C, Knudsen S, Regenberg B, Olsson L, Nielsen J (2005) Improvement of galactose uptake in Saccharomyces cerevisiae through overexpression of phosphoglucomutase: example of transcript analysis as a tool in inverse metabolic engineering. Appl Environ Microbiol 71:6465–6472

    Article  CAS  Google Scholar 

  • Buckeridge MS, Reid JSG (1994) Purification and properties of a novel β-galactosidase or exo-(1→4)-β-d-galactanase from the cotyledons of germinated Lupinus angustifolius L. seeds. Planta 192:502–511

    Article  CAS  Google Scholar 

  • Buckeridge MS, Hutcheon IS, Reid JSG (2005) The role of exo-(1→4)-β-galactanase in the mobilization of polysaccharides from the cotyledon cell walls of Lupinus angustifolius following germination. Ann Bot 96:435–444

    Article  CAS  Google Scholar 

  • Carey AT, Holt K, Picard S, Wilde R, Tucker GA, Bird CR, Schuch W, Seymour GB (1995) Tomato exo-(1→4)-β-d-galactanase. Isolation, changes during ripening in normal and mutant tomato fruit, and characterization of a related cDNA clone. Plant Physiol 108:1099–1107

    Article  CAS  Google Scholar 

  • Carey AT, Smith DL, Harrison E, Bird CR, Gross KC, Seymour GB, Tucker GA (2001) Down-regulation of a ripening-related β-galactosidase gene (TBG1) in transgenic tomato fruits. J Exp Bot 52:663–668

    CAS  Google Scholar 

  • Christgau S, Sandal T, Kofod LV, Dalbøge H (1995) Expression cloning, purification and characterization of a β-1,4-galactanase from Aspergillus aculeatus. Curr Genet 27:135–141

    Article  CAS  Google Scholar 

  • Cid M, Pedersen HL, Kaneko S, Coutinho PM, Henrissat B, Willats WGT, Boraston AB (2010) Recognition of the helical structure of β-1,4-galactan by a new family of carbohydrate-binding modules. J Biol Chem 285:35999–36009

    Article  CAS  Google Scholar 

  • Clarke AE, Anderson RL, Stone BA (1979) Form and function of arabinogalactans and arabinogalactan-proteins. Phytochemistry 18:521–540

    Article  CAS  Google Scholar 

  • Colquhoun IJ, Ralet MC, Thibault JF, Faulds CB, Williamson G (1994) Structure identification of feruloylated oligosaccharides from sugar-beet pulp by NMR spectroscopy. Carbohydr Res 263:243–256

    Article  CAS  Google Scholar 

  • Delangle A, Prouvost AF, Cogez V, Bohin JP, Lacroix JM, Cotte-Pattat NH (2007) Characterization of the Erwinia chrysanthemi Gan locus, involved in galactan catabolism. J Bacteriol 189:7053–7061

    Article  CAS  Google Scholar 

  • De Vries RP, Parenicová L, Hinz SWA, Kester HCM, Beldman G, Benen JAE, Visser J (2002) The β-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions. Eur J Biochem 269:4985–4993

    Article  Google Scholar 

  • Emi S, Fukumoto J, Yamamoto T (1971) Studies of hemicellulolytic enzymes of Bacillus subtilis. Part I. Purification, crystallization and some properties of arabinogalactanase. Agric Biol Chem 35:1891–1898

    Article  CAS  Google Scholar 

  • Emi S, Yamamoto T (1972) Purification and properties of several galactanases of Bacillus subtilis var. amylosacchariticus. Agric Biol Chem 36:1945–1954

    Article  CAS  Google Scholar 

  • Figueroa-Gonzalez I, Quijano G, Ramirez G, Cruz-Guerrero A (2011) Probiotics and prebiotics: perspectives and challenges. J Sci Food Agric 91:1341–1348

    Article  CAS  Google Scholar 

  • Gamauf C, Marchetti M, Kallio J, Puranen T, Vehmaanperä J, Allmaier G, Kubicek CP, Seiboth B (2007) Characterization of the bga1-encoded glycoside hydrolase family 35 β-galactosidase of Hypocrea jecorina with galacto-β-d-galactanase activity. FEBS J 274:1691–1700

    Article  CAS  Google Scholar 

  • Gantulga D, Turan Y, Bevan DR, Esen A (2008) The Arabidopsis At1g45130 and At3g52840 genes encode β-galactosidases with activity toward cell wall polysaccharides. Phytochemistry 69:1661–1670

    Article  CAS  Google Scholar 

  • Gantulga D, Ahn YO, Zhou C, Battogtokh D, Bevan DR, Winkel BSJ, Esen A (2009) Comparative characterization of the Arabidopsis subfamily a1 β-galactosidases. Phytochemistry 70:1999–2009

    Article  CAS  Google Scholar 

  • Gaspar Y, Johnson KL, McKenna JA, Bacic A, Schultz CJ (2001) The complex structures of arabinogalactan-proteins and the journey towards understanding function. Plant Mol Biol 47:161–176

    Article  CAS  Google Scholar 

  • Gosling A, Stevens GW, Barber AR, Kentish SE, Gras SL (2010) Recent advances refining galactooligosaccharide production from lactose. Food Chem 121:307–318

    Article  CAS  Google Scholar 

  • Hashimoto Y (1971) Studies on the enzyme treatment of coffee beans part V. Structure of coffee arabinogalactan. Nippon Nogeikagaku Kaishi 45:147–150

    Article  CAS  Google Scholar 

  • Hashimoto Y, Tsujisaka Y, Fukumoto J (1969) Studies on the enzyme treatment of coffee beans part III. Purification and some properties of galactanase from Rhizopus niveus. Nippon Nogeikagaku Kaishi 43:831–836

    Article  CAS  Google Scholar 

  • Hinz SWA, Pastink MI, van den Broek LAM, Vincken JP, Voragen AGJ (2005a) Bifidobacterium longum endogalactanase liberates galactotriose from type I galactans. Appl Environ Microbiol 71:5501–5510

    Article  CAS  Google Scholar 

  • Hinz SWA, Verhoef R, Schols HA, Vincken JP, Voragen AGJ (2005b) Type I arabinogalactan contains β-d-Galp-(1→3)-β-d-Galp structural elements. Carbohydr Res 340:2135–2143

    Article  CAS  Google Scholar 

  • Hirano Y, Tsumuraya Y, Hashimoto Y (1994) Characterization of spinach leaf α-l-arabinofuranosidases and β-galactosidases and their synergistic action on an endogenous arabinogalactan-protein. Physiol Plant 92:286–296

    Article  CAS  Google Scholar 

  • Hortling B, Tamminen T, Pekkala O (2001) Effects of delignification on residual lignin–carbohydrate complexes in normal pine wood and pine wood enriched in compression wood. 1. Kraft pulping. Nordic Pulp Pap Res J 16:219–224

    Article  CAS  Google Scholar 

  • Huisman MMH, Brül LP, Thomas-Oates JE, Haverkamp J, Schols HA, Voragen AGJ (2001) The occurrence of internal (1→5)-linked arabinofuranose and arabinopyranose residues in arabinogalactan side chains from soybean pectic substances. Carbohydr Res 330:103–114

    Article  CAS  Google Scholar 

  • Husain Q (2010) β Galactosidases and their potential applications: a review. Crit Rev Biotechnol 30:41–62

    Article  CAS  Google Scholar 

  • Hwang J, Pyun YR, Kokini JL (1993) Sidechains of pectins: some thoughts on their role in plant cell walls and foods. Food Hydrocolloids 7:39–53

    Article  CAS  Google Scholar 

  • Ichinose H, Yoshida M, Kotake T, Kuno A, Igarashi K, Tsumuraya Y, Samejima M, Hirabayashi J, Kobayashi H, Kaneko S (2005) An exo-β-1,3-galactanase having a novel β-1,3-galactan-binding module from Phanerochaete chrysosporium. J Biol Chem 280:25820–25829

    Article  CAS  Google Scholar 

  • Ichinose H, Kotake T, Tsumuraya Y, Kaneko S (2006a) Characterization of an exo-β-1,3-d-galactanase from Streptomyces avermitilis NBRC14893 acting on arabinogalactan-proteins. Biosci Biotechnol Biochem 70:2745–2750

    Article  CAS  Google Scholar 

  • Ichinose H, Kuno A, Kotake T, Yoshida M, Sakka K, Hirabayashi J, Tsumuraya Y, Kaneko S (2006b) Characterization of an exo-β-1,3-galactanase from Clostridium thermocellum. Appl Environ Microbiol 72:3515–3523

    Article  CAS  Google Scholar 

  • Ichinose H, Kotake T, Tsumuraya Y, Kaneko S (2008) Characterization of an endo-β-1,6-galactanase from Streptomyces avermitilis NBRC14893. Appl Environ Microbiol 74:2379–2383

    Article  CAS  Google Scholar 

  • Ishimaru M, Smith DL, Mort AJ, Gross KC (2009) Enzymatic activity and substrate specificity of recombinant tomato β-galactosidases 4 and 5. Planta 229:447–456

    Article  CAS  Google Scholar 

  • Kimura I, Yoshioka N, Tajima S (1998) Purification and characterization of an endo-1,4-β-d-galactanase from Aspergillus sojae. J Ferment Bioeng 85:48–52

    Article  CAS  Google Scholar 

  • Knox JP (1995) The extracellular matrix in higher plants. 4. Developmentally regulated proteoglycans and glycoproteins of the plant cell surface. FASEB J 9:1004–1012

    CAS  Google Scholar 

  • Kotake T, Kaneko S, Kubomoto A, Haque MA, Kobayashi H, Tsumuraya Y (2004) Molecular cloning and expression in Escherichia coli of a Trichoderma viride endo-β-(1→6)-galactanase gene. Biochem J 377:749–755

    Article  CAS  Google Scholar 

  • Kotake T, Dina S, Konishi T, Kaneko S, Igarashi K, Samejima M, Watanabe Y, Kimura K, Tsumuraya Y (2005) Molecular cloning of a β-galactosidase from radish that specifically hydrolyzes β-(1→3)- and β-(1→6)-galactosyl residues of arabinogalactan protein. Plant Physiol 138:1563–1576

    Article  CAS  Google Scholar 

  • Kotake T, Kitazawa K, Takata R, Okabe K, Ichinose H, Kaneko S, Tsumuraya Y (2009) Molecular cloning and expression in Pichia pastoris of a Irpex lacteus exo-β-(1→3)-galactanase gene. Biosci Biotechnol Biochem 73:2303–2309

    Article  CAS  Google Scholar 

  • Kotake T, Hirata N, Degi Y, Ishiguro M, Kitazawa K, Takata R, Ichinose H, Kaneko S, Igarashi K, Samejima M, Tsumuraya Y (2011) Endo-β-1,3-galactanase from winter mushroom Flammulina velutipes. J Biol Chem 286:27848–27854

    Article  CAS  Google Scholar 

  • Labavitch JM, Freeman LE, Albersheim P (1976) Structure of plant-cell walls—purification and characterization of a β-1,4-galactanase which degrades a structural component of the primary cell walls of dicots. J Biol Chem 251:5904–5910

    CAS  Google Scholar 

  • Lahaye M, Vigouroux J, Thibault JF (1991) Endo-β-1,4-d-galactanase from Aspergillus niger var. aculeatus: purification and some properties. Carbohydr Polym 15:431–444

    Article  CAS  Google Scholar 

  • Lamsal BP (2012) Production, health aspects and potential food uses of dairy prebiotic galactooligosaccharides. J Sci Food Agric 92:2020–2028

    Article  CAS  Google Scholar 

  • Lazan H, Ng SY, Goh LY, Ali ZM (2004) Papaya β-galactosidase/galactanase isoforms in differential cell wall hydrolysis and fruit softening during ripening. Plant Physiol Biochem 42:847–853

    Article  CAS  Google Scholar 

  • Lee KS, Hong ME, Jung SC, Ha SJ, Yu BJ, Koo HM, Park SM, Seo JH, Kweon DH, Park JC, Jin YS (2011) Improved galactose fermentation of Saccharomyces cerevisiae through inverse metabolic engineering. Biotechnol Bioeng 108:621–631

    Article  CAS  Google Scholar 

  • Le Nours J, Ryttersgaard C, Lo Leggio L, Østergaard PR, Borchert TV, Christensen LLH, Larsen S (2003) Structure of two fungal β-1,4-galactanases: searching for the basis for and pH optimum. Protein Sci 12:1195–1204

    Google Scholar 

  • Ling NXY, Lee J, Ellis M, Liao ML, Mau SL, Guest D, Janssen PH, Kováč P, Bacic A, Pettolino FA (2012) An exo-β-(1→3)-d-galactanase from Streptomyces sp. provides insights into type II arabinogalactan structure. Carbohydr Res 352:70–81

    Article  CAS  Google Scholar 

  • Luonteri E, Laine C, Uusitalo S, Teleman A, Siika-aho M, Tenkanen M (2003) Purification and characterization of Aspergillus β-d-galactanases acting on β-1,4- and β-1,3/6-linked arabinogalactans. Carbohydr Polym 53:155–168

    Article  CAS  Google Scholar 

  • Macfarlane GT, Steed H, Macfarlane S (2008) Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotics. J Appl Microbiol 104:305–344

    CAS  Google Scholar 

  • Manzanares P, de Graaff LH, Visser J (1998) Characterization of galactosidases from Aspergillus niger: purification of a novel α-galactosidase activity. Enzyme Microb Technol 22:383–390

    Article  CAS  Google Scholar 

  • Martín I, Dopico B, Muñoz FJ, Esteban R, Oomen RJFJ, Driouich A, Vincken JP, Visser R, Labrador E (2005) In vivo expression of a Cicer arietinum β-galactosidase in potato tubers leads to a reduction of the galactan side-chains in cell wall pectin. Plant Cell Physiol 46:1613–1622

    Article  Google Scholar 

  • Michalak M, Thomassen LV, Roytio H, Ouwehand AC, Meyer AS, Mikkelsen JD (2012) Expression and characterization of an endo-1,4-β-galactanase from Emericella nidulans in Pichia pastoris for enzymatic design of potentially prebiotic oligosaccharides from potato galactans. Enzyme Microb Technol 50:121–129

    Article  CAS  Google Scholar 

  • Minor JL (1991) Location of lignin-bonded pectic polysaccharides. J Wood Chem Technol 11:159–169

    Article  CAS  Google Scholar 

  • Morita M (1965) Polysaccharides of soybean seeds, part II: a methylated arabinogalactan isolated from methylated product of “hot-water-extract” fraction of soybean seed polysaccharides. Agric Biol Chem 29:626–630

    Article  CAS  Google Scholar 

  • Nakano H, Takenishi S, Watanabe Y (1985) Purification and properties of two galactanases from Penicillium citrinum. Agric Biol Chem 49:3445–3454

    Article  CAS  Google Scholar 

  • Nakano H, Takenishi S, Kitahata S, Kinugasa H, Watanabe Y (1990) Purification and characterization of an exo-1,4-β-galactanase from a strain of Bacillus subtilis. Eur J Biochem 193:61–67

    Article  CAS  Google Scholar 

  • Nakano H, Kitahata S, Kinugasa H, Watanabe Y, Fujimoto H, Ajisaka K, Takenishi S (1991) Transfer reaction catalyzed by exo-β-1,4-galactanase from Bacillus subtilis. Agric Biol Chem 55:2075–2082

    Article  CAS  Google Scholar 

  • Nie SP, Wang C, Cui SW, Wang Q, Xie MY, Phillips GO (2013) A further amendment to the classical core structure of gum arabic (Acacia senegal). Food Hydrocolloids 31:42–48

    Article  CAS  Google Scholar 

  • Nothnagel EA (1997) Proteoglycans and related components in plant cells. Int Rev Cytol 174:195–291

    Article  CAS  Google Scholar 

  • O'Connell Motherway M, Fitzgerald GF, van Sinderen D (2011) Metabolism of a plant derived galactose-containing polysaccharide by Bifidobacterium breve UCC2003. Microb Biotechnol 4:403–416

    Article  Google Scholar 

  • Ohr LM (2010) Health benefits of probiotics and prebiotics. Food Technol 64:59–64

    Google Scholar 

  • Okawa M, Fukamachi K, Tanaka H, Sakamoto T (2013) Identification of an exo-β-1,3-d-galactanase from Fusarium oxysporum and the synergistic effect with related enzymes on degradation of type II arabinogalactan. Appl Microbiol Biotechnol. doi:10.1007/s00253-013-4759-3

    Google Scholar 

  • Okemoto K, Uekita T, Tsumuraya Y, Hashimoto Y, Kasama T (2003) Purification and characterization of an endo-β-(1→6)-galactanase from Trichoderma viride. Carbohydr Res 338:219–230

    Article  CAS  Google Scholar 

  • Ostergaard S, Olsson L, Johnston M, Nielsen J (2000a) Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network. Nat Biotechnol 18:1283–1286

    Article  CAS  Google Scholar 

  • Ostergaard S, Roca C, Rønnow B, Nielsen J, Olsson L (2000b) Physiological studies in aerobic batch cultivations of Saccharomyces cerevisiae strains harboring the MEL1 gene. Biotechnol Bioeng 68:252–259

    Article  CAS  Google Scholar 

  • Pellerin P, Brillouet JM (1994) Purification and properties of an exo-(1→3)-β-d-galactanase from Aspergillus niger. Carbohydr Res 264:281–291

    Article  CAS  Google Scholar 

  • Polizeli MLTM, Rizzatti ACS, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577–591

    Article  CAS  Google Scholar 

  • Ponder GR, Richards GN (1997) Arabinogalactan from Western larch, part III: alkaline degradation revisited, with novel conclusions on molecular structure. Carbohydr Polym 34:251–261

    Article  CAS  Google Scholar 

  • Ralet MC, Thibault JF, Faulds CB, Williamson G (1994) Isolation and purification of feruloylated oligosaccharides from cell walls of sugar-beet pulp. Carbohydr Res 263:227–241

    Article  CAS  Google Scholar 

  • Ross GS, Wegrzyn T, MacRae EA, Redgwell RJ (1994) Apple β-galactosidase. Activity against cell wall polysaccharides and characterization of a related cDNA clone. Plant Physiol 106:521–528

    Google Scholar 

  • Ryttersgaard C, Le Nours J, Lo Leggio L, Jørgensen CT, Christensen LLH, Bjørnvad M, Larsen S (2004) The structure of endo-β-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. J Mol Biol 341:107–117

    Article  CAS  Google Scholar 

  • Sakamoto T, Taniguchi Y, Suzuki S, Ihara H, Kawasaki H (2007) Characterization of Fusarium oxysporum β-1,6-galactanase, an enzyme that hydrolyzes larch wood arabinogalactan. Appl Environ Microbiol 73:3109–3112

    Article  CAS  Google Scholar 

  • Sakamoto T, Tanaka H, Nishimura Y, Ishimaru M, Kasai N (2011) Characterization of an exo-β-1,3-d-galactanase from Sphingomonas sp. 24T and its application to structural analysis of larch wood arabinogalactan. Appl Microbiol Biotechnol 90:1701–1710

    Article  CAS  Google Scholar 

  • Sakamoto T, Nishimura Y, Makino Y, Sunagawa Y, Harada N (2013) Biochemical characterization of a GH53 endo-β-1,4-galactanase and a GH35 exo-β-1,4-galactanase from Penicillium chrysogenum. Appl Microbiol Biotechnol 97:2895–2906

    Article  CAS  Google Scholar 

  • Schultz C, Gilson P, Oxley D, Youl J, Bacic A (1998) GPI-anchors on arabinogalactan-proteins: implications for signalling in plants. Trends Plant Sci 3:426–431

    Article  Google Scholar 

  • Sekimata M, Ogura K, Tsumuraya Y, Hashimoto Y, Yamamoto S (1989) A β-galactosidase from radish (Raphanus sativus L.) seeds. Plant Physiol 90:567–574

    Article  CAS  Google Scholar 

  • Selvendran RR (1983) The chemistry of plant cell walls. In: Birch GG, Parker EJ (eds) Dietary fibre. Applied Science, London, pp 108–113

    Google Scholar 

  • Shipkowski S, Brenchley JE (2006) Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 β-galactosidases are arabinogalactan type I oligomer hydrolases. Appl Environ Microbiol 72:7730–7738

    Article  CAS  Google Scholar 

  • Smith DL, Gross KC (2000) A family of at least seven β-galactosidase genes is expressed during tomato fruit development. Plant Physiol 123:1173–1183

    Article  CAS  Google Scholar 

  • Smith DL, Starrett DA, Gross KC (1998) A gene coding for tomato fruit β-galactosidase II is expressed during fruit ripening. Cloning, characterization, and expression pattern. Plant Physiol 117:417–423

    Article  CAS  Google Scholar 

  • Sørensen SO, Pauly M, Bush M, Skjøt M, McCann MC, Borkhardt B, Ulvskov P (2000) Pectin engineering: modification of potato pectin by in vivo expression of an endo-1,4-β-d-galactanase. Proc Natl Acad Sci USA 97:7639–7644

    Article  Google Scholar 

  • Svetek J, Yadav MP, Nothnagel EA (1999) Presence of a glycosylphosphatidylinositol lipid anchor on rose arabinogalactan proteins. J Biol Chem 274:14724–14733

    Article  CAS  Google Scholar 

  • Tabachnikov O, Shoham Y (2013) Functional characterization of the galactan utilization system of Geobacillus stearothermophilus. FEBS J 280:950–964

    CAS  Google Scholar 

  • Takata R, Tokita K, Mori S, Shimoda R, Harada N, Ichinose H, Kaneko S, Igarashi K, Samejima M, Tsumuraya Y, Kotake T (2010) Degradation of carbohydrate moieties of arabinogalactan-proteins by glycoside hydrolases from Neurospora crassa. Carbohydr Res 345:2516–2522

    Article  CAS  Google Scholar 

  • Trainotti L, Spinello R, Piovan A, Spolaore S, Casadoro G (2001) β-Galactosidases with a lectin-like domain are expressed in strawberry. J Exp Bot 52:1635–1645

    Article  CAS  Google Scholar 

  • Tsumura K, Hashimoto Y, Akiba T, Hirokoshi K (1991) Purification and properties of galactanases from alkalophilic Bacillus sp. S-2 and S-39. Agric Biol Chem 55:1265–1271

    Article  CAS  Google Scholar 

  • Tsumuraya Y, Mochizuki N, Hashimoto Y, Kovác P (1990) Purification of an exo-β-(1→3)-d-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins. J Biol Chem 265:7207–7215

    CAS  Google Scholar 

  • van den Brink J, de Vries RP (2011) Fungal enzyme sets for plant polysaccharide degradation. Appl Microbiol Biotechnol 91:1477–1492

    Article  CAS  Google Scholar 

  • Van der Vlugt-Bergmans CJB, van Ooyen AJJ (1999) Expression cloning in Kluyveromyces lactis. Biotechnol Tech 13:87–92

    Article  Google Scholar 

  • van de Vis JW, Searle-van Leeuwen MJF, Siliha HA, Kormelink FJM, Voragen AGJ (1991) Purification and characterization of endo-1,4-β-d-galactanases from Aspergillus niger and Aspergillus aculeatus: use in combination with arabinases from Aspergillus niger in enzymic conversion of potato arabinogalactan. Carbohydr Polym 16:167–187

    Article  Google Scholar 

  • van Maris AJA, Abbott DA, Bellissimi E, van den Brink J, Kuyper M, Luttik MAH, Wisselink HW, Scheffers WA, van Dijken JP, Pronk JT (2006) Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status. Antonie Van Leeuwenhoek 90:391–418

    Article  CAS  Google Scholar 

  • Voragen AGJ, Coenen GJ, Verhoef RP, Schols H (2009) Pectin, a versatile polysaccharide present in plant cell walls. Struct Chem 20:263–275

    Article  CAS  Google Scholar 

  • Yamaguchi F, Inoue S, Hatanaka C (1995) Purification and properties of endo-β-1,4-galactanase from Aspergillus niger. Biosci Biotechnol Biochem 59:1742–1744

    Article  CAS  Google Scholar 

  • Yang H, Ichinose H, Yoshida M, Nakajima M, Kobayashi H, Kaneko S (2006) Characterization of a thermostable endo-β-1,4-d-galactanase from the hyperthermophile Thermotoga maritima. Biosci Biotechnol Biochem 70:538–541

    Article  CAS  Google Scholar 

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Acknowledgments

This research was partially supported by a grant-in-aid for scientific research (22580091) from the Japan Society for the Promotion of Science.

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  1. Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan

    Tatsuji Sakamoto

  2. Department of Science and Technology on Food Safety, Faculty of Biology-Oriented Science and Technology, Kinki University, Wakayama, 649-6493, Japan

    Megumi Ishimaru

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Sakamoto, T., Ishimaru, M. Peculiarities and applications of galactanolytic enzymes that act on type I and II arabinogalactans. Appl Microbiol Biotechnol 97, 5201–5213 (2013). https://doi.org/10.1007/s00253-013-4946-2

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  • DOI: https://doi.org/10.1007/s00253-013-4946-2

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