Abstract
Inhibitors of 3OC12, an initial signal molecule of the quorum sensing (QS) signaling cascade in Pseudomonas aeruginosa have been developed. Eight inhibitor candidates were synthesized by substituting the head part of 3-oxododecanoyl-homoserine lactone (3OC12) with different aromatic rings, and their docking poses and scores (binding energies) were predicted by in silico modeling study. All compounds gave better docking scores than 3OC12 and good inhibition effects on LasR activity in the in vivo bioassay. Like the modifications in the tail part of 3OC12 in our previous study Kim et al. (2008), the head-part modifications also showed inhibition activity in a fairly good proportion to the docking scores from the modeling analysis. This implies that the head part of 3OC12 also contributes significantly to forming the active conformation of the LasR-3OC12 complex, and its modification could effectively induce the inactive conformation of the complex. We suggest that the head part of 3OC12 is also a good target moiety to develop the structure-based Pseudomonas QS inhibitors.
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Acknowledgments
This research was partially supported by the Korea Ministry of Environment as “The Eco-technopia 21 project (102-081-067)”, the Korea Science and Engineering Foundation grant funded by the Korea government (MOST; No. R01-2007-000-20732-0), and World Class University program through the Korea science and Engineering Foundation funded by the Ministry of Education, Science, and Technology (400-2008-0230). For Joon-Hee Lee, this work was supported by a research grant for the Research Team for Longevity Life Sciences in Pusan National University.
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Kim, C., Kim, J., Park, HY. et al. Structural understanding of quorum-sensing inhibitors by molecular modeling study in Pseudomonas aeruginosa . Appl Microbiol Biotechnol 83, 1095–1103 (2009). https://doi.org/10.1007/s00253-009-1954-3
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DOI: https://doi.org/10.1007/s00253-009-1954-3