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Bioreactor aeration conditions modulate growth and antigen expression during Erysipelothrix rhusiopathiae cultivation

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Abstract

Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, was cultivated in a 5-L stirred and aerated bioreactor under different dissolved oxygen tensions (0%, 5%, and 30% of saturation) for evaluation of the influence of oxygen on cell growth as well as on the production of the main antigenic component of the vaccine against erysipelas, a 64–69 kDa protein (SpaA). The microorganism presented different growth profiles for different aeration conditions. However, at the end of the batch cultivations, similar cell concentrations were obtained under the studied conditions. In order to maximize biomass titers and antigen production, the microorganism was cultivated in fed-batch operation mode under aerobic conditions. Under this condition, there was a fivefold increase in biomass production in comparison to the results attained in batch cultivations. To follow up antigen expression, samples collected during batch cultivations were concentrated and treated with choline for antigen extraction. Antigen expression was then assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by murine immunization tests. It was observed a direct influence of oxygen availability upon antigen expression, which is favored in the presence of oxygen. Analysis of the samples collected throughout the fed-batch process also revealed that antigen production is growth associated.

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Acknowledgements

The authors thank Vallée S.A. for funding this research.

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Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal de São Carlos, Rod. Washington Luís, km 235 (SP 310), 13565-905, São Carlos, SP, Brazil

    Adilson José da Silva, Álvaro de Baptista-Neto, Roberto de Campos Giordano & Teresa Cristina Zangirolami

  2. Vallée S.A., Av. Eng. Luis C. Berrini, 716-2 andar-Brooklin Novo, 04571-000, São Paulo, SP, Brazil

    Maria do Carmo Cilento

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  1. Adilson José da Silva
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  2. Álvaro de Baptista-Neto
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  3. Maria do Carmo Cilento
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  4. Roberto de Campos Giordano
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  5. Teresa Cristina Zangirolami
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Correspondence to Teresa Cristina Zangirolami.

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da Silva, A.J., de Baptista-Neto, Á., do Carmo Cilento, M. et al. Bioreactor aeration conditions modulate growth and antigen expression during Erysipelothrix rhusiopathiae cultivation. Appl Microbiol Biotechnol 79, 23–31 (2008). https://doi.org/10.1007/s00253-008-1399-0

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  • DOI: https://doi.org/10.1007/s00253-008-1399-0

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