Abstract
Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D-xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport.
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References
Archer DB (2000) Filamentous fungi as microbial cell factories for food use. Curr Opin Biotechnol 11:478–483
Bos CJ, Debets AJ, Swart K, Huybers A, Kobus G, Slakhorst SM (1988) Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger. Curr Genet 14:437–443
de Groot MJ, van de Vondervoort PJ, de Vries RP, van Kuyk PA, Ruijter GJ, Visser J (2003) Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism. Microbiology 149:1183–1191
de Groot MJL, Prathumpai W, Visser J, Ruijter GJG (2005) Metabolic control analysis of Aspergillus niger L-arabinose catabolism. Biotechnol Prog 21:1610–1616
Den Haan R, Van Zyl WH (2003) Enhanced xylan degradation and utilisation by Pichia stipitis overproducing fungal xylanolytic enzymes. Enzyme Microb Technol 33:620–628
Dijkema C, Kester HCM, Visser J (1985) 13C NMR studies of carbon metabolism in the hyphal fungus Aspergillus nidulans. Proc Natl Acad Sci U S A 82:14–18
Flipphi M, van de Vondervoort PJ, Ruijter GJ, Visser J, Arst HN Jr, Felenbok B (2003) Onset of carbon catabolite repression in Aspergillus nidulans. Parallel involvement of hexokinase and glucokinase in sugar signaling. J Biol Chem 278:11849–11857
Jansen ML, De Winde JH, Pronk JT (2002) Hxt-carrier-mediated glucose efflux upon exposure of Saccharomyces cerevisiae to excess maltose. Appl Environ Microbiol 68:4259–4265
Jeffries TW, Jin YS (2004) Metabolic engineering for improved fermentation of pentoses by yeasts. Appl Microbiol Biotechnol 63:495–509
Karlgren S, Filipsson C, Mullins JG, Bill RM, Tamas MJ, Hohmann S (2004) Identification of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen. Eur J Biochem 271:771–779
Kelavkar UP, Chhatpar HS (1993) Polyol concentrations in Aspergillus repens grown under salt stress. World J Microbiol Biotechnol 9:579–582
Kötter P, Ciriacy M (1993) Xylose fermentation by Saccharomyces cerevisiae. Appl Microbiol Biotechnol 38:776–783
La Grange DC, Pretorius IS, Claeyssens M, van Zyl WH (2001) Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger beta-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes. Appl Environ Microbiol 67:5512–5519
Prathumpai W, Gabelgaard JB, Wanchanthuek P, van de Vondervoort PJ, de Groot MJ, McIntyre M, Nielsen J (2003) Metabolic control analysis of xylose catabolism in Aspergillus. Biotechnol Prog 19:1136–1141
Ramos AJ, Magan N, Sanchis V (1999) Osmotic and matric potential effects on growth, sclerotia and partitioning of polyols and sugars in colonies and spores of Aspergillus ochraceus. Mycol Res 103:141–147
Richard P, Verho R, Putkonen M, Londesborough J, Penttila M (2003) Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway. FEMS Yeast Res 3:185–189
Ruijter GJG, Visser J, Rinzema A (2004) Polyol accumulation by Aspergillus oryzae at low water activity in solid-state fermentation. Microbiology 150:1095–1101
Tantirungkij M, Nakashima N, Seki T, Yoshida T (1993) Construction of xylose-assimilating Saccharomyces cerevisiae. J Ferment Bioeng 75:83–88
Witteveen CF, Visser J (1995) Polyol pools in Aspergillus niger. FEMS Microbiol Lett 134:57–62
Witteveen CFB, Busink R, van de Vondervoort P, Dijkema C, Swart K, Visser J (1989) L-arabinose and D-xylose catabolism in Aspergillus niger. J Gen Microbiol 135:2163–2171
Acknowledgements
We thank Marieke Escher for technical assistance. This work was supported by grants from the European Union (EUROFUNG), contract numbers BIO4CT96-0535 and QTRK3-199-00729 to J.V.
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The first two authors have contributed equally to this work.
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van de Vondervoort, P.J.I., de Groot, M.J.L., Ruijter, G.J.G. et al. Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport. Appl Microbiol Biotechnol 73, 881–886 (2006). https://doi.org/10.1007/s00253-006-0527-y
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DOI: https://doi.org/10.1007/s00253-006-0527-y