Abstract
In recent years, the use of cynomolgus macaques in biomedical research has increased greatly. However, with the exception of the Mauritian population, knowledge of the MHC class II genetics of the species remains limited. Here, using cDNA cloning and Sanger sequencing, we identified 127 full-length MHC class II alleles in a group of 12 Indonesian and 12 Vietnamese cynomolgus macaques. Forty two of these were completely novel to cynomolgus macaques while 61 extended the sequence of previously identified alleles from partial to full length. This more than doubles the number of full-length cynomolgus macaque MHC class II alleles available in GenBank, significantly expanding the allele library for the species and laying the groundwork for future evolutionary and functional studies.
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Acknowledgments
This work was supported by NIH grant 1 R24 RR021745-01A1. Additional support was provided by NIH grant number P51 RR000167 to the Wisconsin National Primate Research Center, University of Wisconsin–Madison. This research was conducted in part at a facility constructed with support from Research Facilities Improvement Program grant numbers RR15459-01 and RR020141-01. We thank Battelle Biomedical Research Center, the Washington National Primate Research Center, the Cerus Corporation, and the Wake Forest University Primate Center for providing samples. Finally, we acknowledge Nel Otting and Natasja de Groot with the Immuno Polymorphism Database for assigning official allele nomenclature and members of the O’Connor laboratory for their helpful discussions.
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Creager, H.M., Becker, E.A., Sandman, K.K. et al. Characterization of full-length MHC class II sequences in Indonesian and Vietnamese cynomolgus macaques. Immunogenetics 63, 611–618 (2011). https://doi.org/10.1007/s00251-011-0537-5
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DOI: https://doi.org/10.1007/s00251-011-0537-5