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Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations

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Abstract

Cynomolgus monkey is one of the macaque species currently used as an animal model for experimental surgery and medicine, in particular, to experiment new drugs or therapy protocols designed for the prevention of allograft rejection. In this field, it is of utmost importance to select histoincompatible recipient–donor pairs. One way to ensure incompatibility between donor and recipient is to check their major histocompatibility complex (MHC) genotypes at the loci playing a determinant role in histocompatibility. We report in this paper on the cynomolgus monkey DRB polymorphism evidenced by sequencing of amplified exon 2 separated either by denaturing gradient gel electrophoresis (DGGE), or by cloning. By the study of 253 unrelated animals from two populations (Mauritius and The Philippines), we characterized 50 exon 2 sequences among which 28 were identical to sequences already reported in Macaca fascicularis or other macaque species (Macaca mulatta, Macaca nemestrina). By cloning and sequencing DRB cDNA, we revealed two additional DRB alleles. Out of the 20 haplotypes that we defined here, only two were found in both populations. The functional impact of DR incompatibility was studied in vitro by mixed lymphocyte culture.

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Acknowledgements

We thank Béatrice Atlan, Stéphanie Despiau and Sylvie Molinier for their excellent technical assistance. We thank Alexandrine Canitrot for her contribution to this study during her master’s degree. This study was supported by the Ministère Français de la Recherche (Contract E.A. 3034), and by research funds from Novartis Pharma AG. We thank Nastasia de Groot and Ronald Bontrop to have assigned DRB allele names and included new alleles in the IMGT/MHC-NHP database.

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Authors and Affiliations

  1. Laboratoire d’Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France

    Antoine Blancher, Pierre Tisseyre, Marianne Dutaur, Pol-André Apoil & Michel Abbal

  2. Laboratoire d’immunologie, Hopital Rangueil, TSA 50032, Toulouse, 31059, cedex 9, France

    Antoine Blancher, Pol-André Apoil & Michel Abbal

  3. Transplantation Research, Novartis Pharma AG, WSJ-386.1.55A, Basel, CH-4002, Switzerland

    Claudine Maurer, Friedrich Raulf & Marc Bigaud

  4. Institut de Transgénose, CNRS-GEM FRE 2358, 3B, rue de la Férollerie, F-45071, Orléans, cedex 2, France

    Valérie Quesniaux

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  1. Antoine Blancher
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  2. Pierre Tisseyre
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  3. Marianne Dutaur
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  4. Pol-André Apoil
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  5. Claudine Maurer
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  6. Valérie Quesniaux
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  7. Friedrich Raulf
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  8. Marc Bigaud
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  9. Michel Abbal
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Correspondence to Antoine Blancher.

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Blancher, A., Tisseyre, P., Dutaur, M. et al. Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations. Immunogenetics 58, 269–282 (2006). https://doi.org/10.1007/s00251-006-0102-9

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  • DOI: https://doi.org/10.1007/s00251-006-0102-9

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