Abstract
Major histocompatibility complex (MHC) class I alleles of nonhuman primates have been associated with disease susceptibility, resistance, and resolution. Here, using high-resolution pyrosequencing, we characterized MHC class I transcripts expressed in Mauritian cynomolgus macaques (MCM), a nonhuman primate population with restricted MHC diversity. Using this approach, we identified 67 distinct MHC class I transcripts encoded by the seven most frequent MCM MHC class I haplotypes, 40 (60%) of which span the complete open reading frames. These results double the number of MHC class I sequences previously defined by cloning and Sanger sequencing of cDNA-PCR products and provide a rapid, high-throughput, and economical method for MHC characterization. Overall, this approach significantly expanded our knowledge of MCM haplotypes and will facilitate future studies on disease pathogenesis and protective cellular immunity.
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Acknowledgments
We would like to thank 454 Life Sciences, a Roche Company, for providing early access to Titanium amplicon chemistry and the GS Junior instrument. We would also like to thank Benjamin Bimber and Simon Lank for their bioinformatics support. This research was supported by the US National Institute of Allergy and Infectious Disease contract number HHSN266200400088C/N01-AI-40088 at the Wisconsin National Primate Research Center, a facility supported by grant number P51 RR000167 from the National Center for Research Resources, a component of the National Institutes of Health. Work from this manuscript was conducted in a facility constructed with support from Research Facilities Improvement Program Grant numbers RR15459-01 and RR020141-01.
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Budde, M.L., Wiseman, R.W., Karl, J.A. et al. Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing. Immunogenetics 62, 773–780 (2010). https://doi.org/10.1007/s00251-010-0481-9
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DOI: https://doi.org/10.1007/s00251-010-0481-9