Skip to main content

Advertisement

SpringerLink
Log in

Characterization and expression of a CD40 homolog gene in Japanese flounder Paralichthys olivaceus

  • Original Paper
  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

A CD40 homolog cDNA encoding 300 amino acid residues was isolated from a Japanese flounder leukocyte cDNA library. The amino acid sequence identities of Japanese flounder CD40 and previously reported CD40s of cow, human, mouse, and chicken range from 32 to 35%. The positions of cysteine residues, CD40 ligand binding amino acid residues, and four cysteine-rich domains are well conserved in Japanese flounder CD40. The Japanese flounder CD40 gene is composed of nine exons and eight intervening introns spread over 6 kb. The nucleotide sequence of the 5′-flanking region of this gene revealed the presence of several regulatory regions, including a TATA-like box, AP-1-, CEBPB-, IRF-1-, LYF-1-, NF-κBp-, SP-1-, and Stat-1-like motifs. In healthy fish, reverse transcriptase–polymerase chain reaction (RT-PCR) detected constitutive expression of CD40 in all tested tissues (leukocytes, kidney, spleen, liver, intestine, brain, gill, and skin). The mRNA of CD40 was predominantly expressed in several tissues that contained lymphocytes (leukocytes, kidney, spleen, intestine, and gill). Expression of the Japanese flounder CD40 molecule was induced in peripheral blood leukocytes from 1 to 6 h following Con A (50 μg/ml)/phorbol myristate acetate (PMA) (0.35 μg/ml) stimulation, with a peak at 1 h after stimulation, and increased at 1, 3, and 6 h after induction by lipopolysaccharide (LPS) (500 μg/ml) compared with the control.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman EW (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  PubMed  CAS  Google Scholar 

  • Altschul SF, Madden TL, Schofer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Aoki T, Nam B-H, Hirono I (1999) Sequence of 596 cDNA clones (565,977) of Japanese flounder Paralichthys olivaceus leukocytes infected with hirame rhabdovirus. Mar Biotechnol 1:477–488

    Article  PubMed  CAS  Google Scholar 

  • Bajorath J, Chalupny NJ, Marken JS, Siadak AW, Skonier J, Gordon M, Hollenbaugh D, Noelle RJ, Ochs HD, Aruffo A (1995) Identification of residues on CD40 and its ligand which are critical for the receptor–ligand interaction. Biochemistry 34:1833–1844

    Article  PubMed  CAS  Google Scholar 

  • Braesch-Andersen S, Paulie S, Koho H, Nika H, Aspenstrom P, Perlmann P (1989) Biochemical characteristics and partial amino acid sequence of the receptor-like human B cell and carcinoma antigen CDw40. J Immunol 142:562–567

    PubMed  CAS  Google Scholar 

  • Clark EA, Ledbetter JA (1986) Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50. Proc Natl Acad Sci U S A 83:4494–4498

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1996) PHYLIP (phylogeny inference package), version 4.0. Department of Genetics, University of Washington, Seattle

    Google Scholar 

  • Fries KM, Sempowski GD, Gaspari AA, Blieden T, Looney RJ, Phipps RP (1995) CD40 expression by human fibroblasts. Clin Immunol Immunopathol 77:42–51

    Article  PubMed  CAS  Google Scholar 

  • Galy AH, Spits H (1992) CD40 is functionally expressed on human thymic epithelial cells. J Immunol 149:775–782

    PubMed  CAS  Google Scholar 

  • Graham S, Secombes CJ (1988) The production of a macrophage-activating factor from rainbow trout Salmo gairdneri leucocytes. Immunology 65:293–297

    PubMed  CAS  Google Scholar 

  • Hart DN, McKenzie JL (1988) Isolation and characterization of human tonsil dendritic cells. J Exp Med 168:157–170

    Article  PubMed  CAS  Google Scholar 

  • Hirono I, Nam B-H, Kurobe T, Aoki T (2000) Molecular cloning, characterization, and expression of TNF cDNA and gene from Japanese flounder Paralichthys olivaceus. J Immunol 165:4423–4427

    PubMed  CAS  Google Scholar 

  • Johnson MD, Housey GM, Kirschmeier PT, Weinstein IB (1987) Molecular cloning of gene sequences regulated by tumor promoters and mitogens through protein kinase C. Mol Cell Biol 7:2821–2829

    PubMed  CAS  Google Scholar 

  • Karmann K, Hughes CC, Schechner J, Fanslow WC, Pober JS (1995) CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression. Proc Natl Acad Sci U S A 92:4342–4346

    Article  PubMed  CAS  Google Scholar 

  • Katagiri T, Asakawa S, Hirono I, Aoki T, Shimizu N (2000) Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus. Mar Biotechnol 2:571–576

    Article  PubMed  CAS  Google Scholar 

  • Kooten C, Banchereau J (2000) CD40-CD40 ligand. J Leukoc Biol 67:2–17

    PubMed  Google Scholar 

  • Krzesz R, Wagner AH, Cattaruzza M, Hecker M (1999) Cytokine-inducible CD40 gene expression in vascular smooth muscle cells is mediated by nuclear factor κB and signal transducer and activator of transcription-1. FEBS Lett 453:191–196

    Article  PubMed  CAS  Google Scholar 

  • Murphy JJ, Norton JD (1993) Phorbol ester induction of early response gene expression in lymphocytic leukemia and normal human B cells. Leuk Res 17:657–662

    Article  PubMed  CAS  Google Scholar 

  • Nam B-H, Yamamoto E, Hirono I, Aoki T (2000) A survey of expressed genes in the leukocytes of Japanese flounder, Paralichthys olivaceus, infected with hirame rhabdovirus. Dev Comp Immunol 24:13–24

    Article  PubMed  CAS  Google Scholar 

  • Ota N, Nakajima T, Takeuchi T, Shirai Y, Emi M (1999) A highly polymorphic CA repeat marker at the interleukin-11 locus. Genes Immun 1:159–160

    Article  PubMed  CAS  Google Scholar 

  • Park C-I, Kurobe T, Hirono I, Aoki T (2003) Cloning and characterization of cDNA for two distinct tumor necrosis factor receptor genes from Japanese flounder Paralichthys olivaceus. Dev Comp Immunol 27:365–375

    Article  PubMed  CAS  Google Scholar 

  • Ridge JP, Rosa F, Matzinger P (1998) A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell. Nature 393:474–478

    Article  PubMed  CAS  Google Scholar 

  • Schönbeck U, Libby P (2001) The CD40/CD15 receptor/ligand dyad. Cell Mol Life Sci 58:4–43

    Article  PubMed  Google Scholar 

  • Schönbeck U, Mach M, Bonnefoy JY, Loppnow H, Flad HD, Libby P (1997) Ligation of CD40 activates interleukin 1β-converting enzyme (caspase-1) activity in vascular smooth muscle and endothelial cells and promotes elaboration of active interleukin 1β. J Biol Chem 272:19569–19574

    Article  PubMed  Google Scholar 

  • Sempowski GD, Chess PR, Moretti AJ, Padilla J, Phipps RP, Blieden TM (1997) CD40 mediated activation of gingival and periodontal ligament fibroblasts. J Periodontol 68:284–292

    PubMed  CAS  Google Scholar 

  • Stamenkovic I, Clark EA, Seed B (1989) A B lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas. EMBO J 8:1403–1410

    PubMed  CAS  Google Scholar 

  • Takeshita T, Goto Y, Nakamura M, Fujii M, Iwami M, Hinuma Y, Sugamura K (1988) Phorbol esters can persistently replace interleukin-2 (IL-2) for the growth of a human IL-2-dependent T-cell line. J Cell Physiol 136:319–325

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported in part by grants-in-aid for Scientific Research (S) (no.15108003) from the Ministry of Education, Science, Sports and Culture of Japan, and a research grant of the JSPS.

Author information

Author notes

  1. Chan-Il Park

    Present address: Department of Marine Biology and Agriculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong, 650-160, South Korea

Authors and Affiliations

  1. Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo, 108-8477, Japan

    Chan-Il Park, Ikuo Hirono, Jee Youn Hwang & Takashi Aoki

Authors
  1. Chan-Il Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ikuo Hirono
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jee Youn Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takashi Aoki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takashi Aoki.

Additional information

The nucleotide sequence data reported in this paper have been submitted to the DDBJ nucleotide database and have been assigned the accession number BAC87848 (CD40 cDNA)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, CI., Hirono, I., Hwang, J.Y. et al. Characterization and expression of a CD40 homolog gene in Japanese flounder Paralichthys olivaceus . Immunogenetics 57, 682–689 (2005). https://doi.org/10.1007/s00251-005-0032-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00251-005-0032-y

Keywords

Search

Navigation