Skip to main content
SpringerLink
Log in

Genomic Bacterial Artificial Chromosome Library of the Japanese Flounder Paralichthys olivaceus

  • Published:
Marine Biotechnology Aims and scope Submit manuscript

Abstract:

We have constructed a genomic bacterial artificial chromosome (BAC) library from homozygous cloned Japanese flounder Paralichthys olivaceus using the pBAC-lac vector. This BAC library consists of about 49,100 clones and is deposited in 128 microtiter plates with 384 wells. The average size of inserted DNA was calculated to be 165 kb. The BAC library was determined to cover 9 times the Japanese flounder haploid genome. The Japanese flounder genomic BAC library will be useful for gene isolation as well as quantitative trait loci (QTL) analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Laboratory of Genetics and Biochemistry, Tokyo University of Fisheries, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan, , , , , , JP

    Takayuki Katagiri, Ikuo Hirono & Takashi Aoki

  2. Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan, , , , , , JP

    Shuichi Asakawa & Nobuyoshi Shimizu

Authors
  1. Takayuki Katagiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuichi Asakawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ikuo Hirono
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takashi Aoki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nobuyoshi Shimizu
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received March 1, 2000; accepted May 29, 2000.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Katagiri, T., Asakawa, S., Hirono, I. et al. Genomic Bacterial Artificial Chromosome Library of the Japanese Flounder Paralichthys olivaceus . Mar. Biotechnol. 2, 571–576 (2000). https://doi.org/10.1007/s101260000041

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s101260000041

Search

Navigation