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Bacterial Community Structure of Autotrophic Denitrification Biocathode by 454 Pyrosequencing of the 16S rRNA Gene

  • Environmental Microbiology
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Abstract

Few studies have been conducted to explore the community composition in denitrifying biocathode. Herein, the microbial communities of denitrifying biocathodes yielding current of 1 mA (reactor C1) and 1.5 mA (reactor C2) were characterized by 454 pyrosequencing. The nitrate removal efficiencies in C1 and C2 were about 93 and 85 %, respectively. The optimization of data generated high-quality sequences of 18509 in C1 and 14857 in C2. Proteobacteria was the predominant phylum, and Bacteroidetes, Chloroflexi, and Planctomycetes were the subdominant groups. Classes of Alphaproteobacteria, Anaerolineae, and Phycisphaerae may benefit the performance of current production and nitrate removal. Twenty-nine dominant operational taxonomic units (OTUs) accounted for 64 and 65 % of sequences in C1 and C2, respectively. A denitrifying pathway was constructed based on the phylogenetic analysis and function inferring of the dominant OTUs. Obviously, the 454 pyrosequencing provided a high-resolution profile of bacteria community in denitrifying biocathode.

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Acknowledgments

This study was sponsored by the National Natural Science Foundation of China (51208490, 21177122) and the Natural Science Foundation of Fujian Province (2012J05105).

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Authors and Affiliations

  1. Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China

    Yong Xiao, Yue Zheng, Song Wu & Feng Zhao

  2. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China

    Yue Zheng, Song Wu & Zhao-Hui Yang

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  1. Yong Xiao
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  2. Yue Zheng
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  3. Song Wu
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  4. Zhao-Hui Yang
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  5. Feng Zhao
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Corresponding authors

Correspondence to Yong Xiao or Feng Zhao.

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Xiao, Y., Zheng, Y., Wu, S. et al. Bacterial Community Structure of Autotrophic Denitrification Biocathode by 454 Pyrosequencing of the 16S rRNA Gene. Microb Ecol 69, 492–499 (2015). https://doi.org/10.1007/s00248-014-0492-4

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  • DOI: https://doi.org/10.1007/s00248-014-0492-4

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