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Role for the TRPV1 Channel in Insulin Secretion from Pancreatic Beta Cells

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Abstract

Transient receptor potential channels have been put forward as regulators of insulin secretion. A role for the TRPV1 ion channel in insulin secretion has been suggested in pancreatic beta cell lines. We explored whether TRPV1 is functionally expressed in RINm5F and primary beta cells from neonate and adult rats. We examined if capsaicin could activate cationic non-selective currents. Our results show that TRPV1 channels are not functional in insulin-secreting cells, since capsaicin did not produce current activation, not even under culture conditions known to induce the expression of other ion channels in these cells. Although TRPV1 channels seem to be irrelevant for the physiology of isolated beta cells, they may play a role in glucose homeostasis acting through the nerve fibers that regulate islet function. At the physiological level, we observed that Trpv1 −/− mice presented lower fasting insulin levels than their wild-type littermates, however, we did not find differences between these experimental groups nor in the glucose tolerance test or in the insulin secretion. However, we did find that the Trpv1 −/− mice exhibited a higher insulin sensitivity compared to their wild-type counterparts. Our results demonstrate that TRPV1 does not contribute to glucose-induced insulin secretion in beta cells as was previously thought, but it is possible that it may control insulin sensitivity.

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Acknowledgments

We are grateful to Dr. Clara Ortega for providing the RINm5F cells. To Ana María Escalante-Gonzalbo and Francisco Pérez-Eugenio, from the Computer Unit, and to Dr. Fernando García from the Microscopy Unit for excellent technical support. Also to Dr. Claudia Rivera-Cerecedo, Dr. Héctor Malagón and the bioterium staff. All from Instituto de Fisiología Celular, UNAM. Work in Dr. Hiriart laboratory was supported by grants from Consejo Nacional de Ciencia y Tecnología CONACYT CB2009-131647, and DGAPA-PAPIIT IN215611, Universidad Nacional Autónoma de México (UNAM). C. M. Diaz-Garcia is a PhD student from Posgrado en Ciencias Biológicas, UNAM and was supported by a doctoral fellowship from CONACyT. Work in Dr. Rosenbaum laboratory was supported by grants from the Marcos Moshinsky Foundation, CONACyT 129474, DGAPA-PAPIIT IN204111 and IN200314. S. L. Morales-Lázaro is supported by the postdoctoral scholarship program of UNAM (DGAPA).

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  1. División de Neurociencias, Departamento de Neurodesarrollo y Fisiología, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior S/N Avenida Universidad, Ciudad Universitaria, CP 04510, Coyoacán, Mexico DF, Mexico

    Carlos Manlio Diaz-Garcia, Sara L. Morales-Lázaro, Carmen Sánchez-Soto, Myrian Velasco, Tamara Rosenbaum & Marcia Hiriart

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  1. Carlos Manlio Diaz-Garcia
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  2. Sara L. Morales-Lázaro
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  3. Carmen Sánchez-Soto
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  5. Tamara Rosenbaum
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  6. Marcia Hiriart
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Correspondence to Marcia Hiriart.

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Diaz-Garcia, C.M., Morales-Lázaro, S.L., Sánchez-Soto, C. et al. Role for the TRPV1 Channel in Insulin Secretion from Pancreatic Beta Cells. J Membrane Biol 247, 479–491 (2014). https://doi.org/10.1007/s00232-014-9658-8

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