Abstract
Ecosystem engineers often exert strong effects on the recruitment of other species through modification of the local abiotic and biotic environment. In 2015, artificial reefs in eastern Tasmania (− 42.64693, 148.01481) spanning seven different patch sizes (0.12–7.68 m2) and supporting four densities of transplanted kelp (Ecklonia radiata at 0, 4.1, 8.2 and 16.4 kelp m−2) were used to determine how the patch size and density of this ecosystem engineer influenced the recruitment of microphytobenthic (MPB) algae, and a secondary ecosystem engineer, the mussel Mytilus galloprovincialis. Increasing kelp density and patch size inhibited the establishment of subcanopy MPB algae on settlement slides and reduced the recruitment of mussels in standardised rope fibre habitats (RFHs). The productivity:biomass ratio (P:B) of mussel recruits tended to be lower on small reefs and reefs without kelp, relative to larger reefs with high densities of kelp. Canopy shading and reduced cover of turf algae appeared to negatively impact the recruitment of MPB algae and mussels, whilst reduced sediment accumulation on the reefs due to the kelp was also negatively associated with mussel recruitment. These findings highlight the role of ecosystem engineering by kelp in inhibiting the establishment of other species which may additionally impact community dynamics and primary and secondary productivity. The limited capacity of small kelp patches to inhibit the recruitment of other organisms supports the notion that fragmented patches of ecosystem engineers could be more suspectable to adverse outcomes from species interactions making them less resistant to shifts towards an alternative ecosystem state.
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Introduction
Ecosystem engineers often exert strong effects on the recruitment of other species through their capacity to modify local abiotic and biotic environmental conditions (Lambrinos and Bando 2008; Wright et al. 2016). Understanding the consequences of variability in the structure of ecosystem engineers (i.e., their patch size and density) on the local environment and the establishment of associated species can help us anticipate the impacts of habitat degradation (i.e., habitat loss or fragmentation) or predict the benefits of ecological restoration (Crain and Bertness 2006).
Forests of canopy-forming kelp, which dominate rocky reefs in temperate and subpolar coastal waters around the world, face a barrage of stresses (e.g., ocean warming, range expanding herbivores, coastal development) which are causing precipitous declines in canopy cover in some locations (Steneck et al. 2002; Johnson et al. 2011; Wernberg et al. 2013; Krumhansl et al. 2016; Vergés et al. 2016). Restoring kelp forests, which in some instances requires use of artificial structures to provide substratum for the kelp to grow, provides an avenue to reverse these declines (Reed et al. 2006; Wood et al. 2019; Layton et al. 2020).
Kelp canopy cover substantially reduces the transmission of ambient light to the benthos whist increasing rates of particle deposition to the substratum and baffling water flow beneath the canopy (Eckman et al. 1989; Wernberg et al. 2005; Layton et al. 2019b). The laminae of many smaller kelp species also sweep and scour the benthos which removes sediment and increases particle re-suspension (Toohey et al. 2004; Teagle et al. 2017). High kelp cover typically results in less understory algae and associated epifauna, whilst increasing the prevalence of sessile invertebrates (Arkema et al. 2009; Flukes et al. 2014; Miller et al. 2018; Shelamoff et al. 2019b, 2020a). The physical structure of the kelp itself can provide habitat complexity which supports some larger mobile species such as fishes and macroinvertebrates (Efird and Konar 2014; Hinojosa et al. 2014; Bruno et al. 2018; Shelamoff et al. 2020b). Kelp additionally provides a range of physical and chemical cues which may enhance or discourage the recruitment of some organisms (Denley et al. 2014; Hinojosa et al. 2014). Furthermore, by directly influencing the abundance of various species, kelp also modify trophic and competitive interactions within the community, additionally affecting other species and community composition (Graham 2004; Arkema et al. 2009; Miller et al. 2018). In particular, canopy-shading and abrasion from kelp blades impedes the growth of understory turf algae which can otherwise outcompete more structurally diverse foliose algal species as well as filter feeding sessile invertebrates resulting in a less productive and diverse assemblage overall (Strain et al. 2014; Filbee-Dexter and Wernberg 2018). Highly persistent algal turfs are sometimes viewed as an alternative ecosystem state to kelp forests (Reeves et al. 2018). Turfs trap sediments and can modify chemical boundary layers (Layton et al. 2019a); however, some species thrive amongst the highly branched filamentous algae and the entrapped sediments that characterise these habitats (Connell et al. 2014; Fraser et al. 2020; Shelamoff et al. 2020a).
Abiotic and biotic ecosystem engineering by kelp is likely to have important implications for the recruitment of microphytobenthic (MPB) organisms (e.g., bacteria, cyanobacteria, benthic diatoms, other microalgae, and macroalgal spores), which are important primary producers beneath the kelp canopy. These organisms have a pivotal role at the base of coastal food webs in the cycling of carbon and nutrients, and additionally in stabilising sediments (Moncreiff and Sullivan 2001; Chang-Keun et al. 2006; Frankenbach et al. 2020; Hope et al. 2020). In kelp associated food webs, epilithic and epiphytic MPB species are a major food source for epifauna which then support the productivity of fishes and macroinvertebrates (Fraser et al. 2020; Shelamoff et al. 2020a). MPB organisms also facilitate the recruitment of many other species through the production of chemical cues which attract and induce settlement and metamorphosis of larvae, thus providing a further critical ecosystem function (Peteiro et al. 2007; Leise et al. 2009). Although spatially variable in composition, MPB organisms are highly abundant within sediments and on other benthic surfaces and are therefore likely to readily colonise available substratum from the immediate surrounds (Jackson et al. 2010).
On intertidal rocky shores, relatively small macroalgae such as Pyropia sp. and Fucus sp. positively influence the establishment of MPB algae by providing a refuge from the harsh physical environment (i.e., heat, desiccation, wave action, predation) and by helping to concentrate nutrients beneath their canopy (Sundbäck and McGlathery 2005; Umanzor et al. 2017, 2018). It is less clear whether MPB algae are influenced by the structure of canopy-forming kelp subtidally. Light limitation, especially beneath a dense kelp canopy, could impede the recruitment and growth of MPB algae through reducing photosynthesis. Turf algae beneath a more open canopy on the other hand, may allow for MPB organisms to flourish by providing a relatively light rich environment (i.e., no canopy shading), a high surface area on which to grow, and additional habitat in the form of sediments which co-occur with turf-algae (MacIntyre et al. 1996; Totti et al. 2009). There are however a range of additional factors such as surface stability, roughness, sediment characteristics, wave energy, and bulk water flow that vary depending on the amount of kelp or turf algae on reefs, and which could ultimately affect the establishment and composition of MPB organisms (Jantzen et al. 2013; Semcheski et al. 2016).
Kelp are also likely to influence the establishment of benthic macroinvertebrates including shellfish that can form extensive and complex three-dimensional reef structures and provide a range of additional ecosystem services including nutrient removal, microhabitat provision and modification, additional trophic interactions and pathways of energy flow, and fisheries enhancement (Nielsen et al. 2016; Fitzsimons et al. 2019; Rullens et al. 2019). Many shellfish reefs have been lost across the globe through over-exploitation, poor water quality, and other forms of habitat degradation which has prompted substantial efforts to restore these valuable ecosystems (Beck et al. 2011; Fitzsimons et al. 2019). Recruiting shellfish may respond either positively or negatively to macroalgal cover, which has implications for restoration practice (Witman 1987; Bulleri et al. 2006; Yang et al. 2007; Kochmann and Crowe 2014). The facilitatory effect of some macroalgae on shellfish recruitment may help to support the establishment of shellfish reefs whilst providing additional and complementary ecosystem services (Shelamoff et al. 2019b; McAfee et al. 2020; Reeves et al. 2020). Conversely, if macroalgae interact competitively with shellfish (Witman 1987; Chapman et al. 2005), this is likely to hinder shellfish restoration efforts. It is currently unclear how canopy-forming kelp will affect the establishment of many shellfish species.
Mussels are often a focal species in shellfish reef restoration efforts with a number of factors likely to influence their recruitment. Their larvae seek suitable habitats in which to settle and metamorphose. Choice of settlement location has been shown to be positively influenced by the presence and abundance of MPB biofilms (Bao et al. 2007; Peteiro et al. 2007; Yang et al. 2017), but may be negatively affected by other factors such as the presence of conspecific settlers (von der Meden et al. 2010). Thin macroalgal filaments can also provide an initial attachment point for settling mussel larvae and rougher surface textures may additionally increase larval settlement (Petraitis 1990). Following initial settlement, mussel larvae may subsequently move location through gliding along the substratum or through substratum de-attachment and re-settlement prior to metamorphosis. After metamorphosis mussels may further re-locate through the detachment and reattachment of their byssal threads. For example, Mytilus edulis initially settles in areas of low flow, but can subsequently migrate to areas of high flow which better supports their filter feeding (Dobretsov and Wahl 2008). Small patches of reef and edge locations with higher water flow may offer improved environmental conditions for mussel growth and survival, however these locations may be more difficult to settle into and more exposed to predators (Svane and Ompi 1993). Despite the importance of filamentous algae for the settlement of some mussel species, it is unclear whether turf-dominated reefs (comprised mainly of highly branched and filamentous algae) that often occur in the absence of kelp enhance mussel settlement or improve survival prospects relative to the subcanopy environment of a kelp forest.
Here we aimed to determine the role of the patch size and density of Australasia’s most widespread kelp species, Ecklonia radiata, on the recruitment of MPB algae and mussels. We then determined how these patch characteristics influenced the productivity to biomass ratio (P:B) of the mussels that recruited to the reefs. We also assessed how the recruitment and P:B of mussels were affected by ‘edge’ vs. ‘interior’ patch positions. Finally, we explored the potential role of abiotic and biotic correlates (subcanopy: light, water flow, sediment deposition, the depth of accumulated sediments, and the percentage cover of turf algae) in explaining the abundance of MPB algae and mussel recruits (in the centre of patches). Overall, we predicted that increasing kelp patch size and canopy density would 1) reduce the development of MPB algae predominantly due to shading and 2) reduce recruitment of mussels as a result of water flow reductions and/or scour, but increase annual P:B of mussels. Mussels were also predicted to be less abundant and have a higher P:B, in central positions on the reefs compared to edge positions due to reduced adverse effects of kelp at the patch edge. Thus, small reefs and those without a sufficiently dense kelp canopy and which were dominated by turf algae (Shelamoff et al. 2019b), were expected to support relatively high abundances of MPB algae and mussels.
Materials and methods
Artificial reefs
This experiment used 28 artificial reefs supporting transplants of Ecklonia radiata, the dominant canopy-forming kelp across southern Australia, fully described in Layton et al. (2019b). To summarise, the experimental reefs were deployed in December 2014 and were constructed of concrete pavers supported on a steel frame elevated 30 cm above sandy substratum, spanning a range of sizes: 0.12, 0.24, 0.48, 1.08, 1.92, 4.32, and 7.68 m2 (Fig. 1a). Adult E. radiata were transplanted to the pavers at four transplant densities (0, 4.1, 8.2, and 16.4 kelp m−2), hereafter referred to as: zero, low, medium and high densities, respectively. These densities were maintained for the duration of these experiments by replacing any losses with fresh transplant material at 6 week intervals. The medium density was the density of kelps on the natural reef where they were collected.
MPB recruitment
We positioned a single settlement slide made of polycarbonate (25 × 75 × 3 mm) near the mid-point of every experimental reef in October to November 2015 (we anticipated more rapid establishment of MPB algae for warmer/longer day length times of the year) to determine the effects of E. radiata structure on the abundance of MPB algae (Fig. 1b). Each slide was previously scraped using sandpaper (#550) to provide a rough surface to increase settlement. After 17 days on the reefs, slides were removed and individually placed into zip lock bags with ample seawater for transportation. Once in the laboratory, every slide was transferred to a plastic petri dish where they were preserved with Lugol’s solution (1%). To quantify and identify MPB cells, slides were visually divided into ten equally sized sections. One haphazardly positioned photograph was taken from each of eight randomly chosen sections (avoiding the edges) under 40 × magnification to yield eight images per slide and within each image cells were identified and quantified identified using Image J (Schneider 2012).
Mussels
Recruitment
The recruitment of the mussel Mytilus galloprovincialis was assessed using standardised rope fibre habitats (RFHs) attached to the pavers in the centre and at the northern (light exposed) edge of each reef (to determine differences between an interior and a relatively consistent edge environment) between May 2015 and November 2015 (with high recruitment expected towards the end of this period) (Fig. 1a). A detailed description of the RFHs is given in Shelamoff et al. (2020a). After a 6-month deployment period, the RFHs were collected and brought to the lab for analysis. Mussels were enumerated after teasing the rope fibres apart using tweezers.
P:B
To understand variability in habitat quality across the different reefs in supporting a standing biomass of mussels, we assessed productivity to biomass ratio (P:B) by examining the size structure of mussels associated with the RFHs. Here we used P:B ratios to indicate the quality of habitat for mussel growth, with low P:B values (a higher proportion of large individuals) indicating more beneficial habitats for mussel establishment relative to habitats with a higher P:B (a higher proportion of small individuals). We used the method outlined in Edgar (1990) based on size structure to estimate productivity and biomass and therefore P:B of the mussel recruits. This involved separating mussels into different size classes using a stacked series of sieves (1.0, 1.4, 2.0, 2.8, 4.0 mm aperture sizes) after their removal from the RFHs. We then used the abundance of mussels in each size class to estimate biomass (based on previously established relationships between size and biomass from the literature) (Edgar 1990). Productivity (P) was estimated as: P = 0.0049 × biomass0.80 × temperature0.89 and P:B determined accordingly (Edgar 1990) (water temperature at the time of collection was 14 °C).
Environmental correlates
We used average annual data from Layton et al. (2019b) (light, water flow, sediment deposition, and sediment accumulation) and Shelamoff et al. (2019b) (percentage cover of turf algae) as quantitative descriptors of the abiotic and biotic environment for each experimental reefs beneath the canopy (on reefs where a canopy existed), and correlated these measures with recruitment of MPB algae (to polycarbonate slides) and mussels (to RFHs) in the centre of reefs only (as the abiotic and biotic measurements were indicative of the internal environment of the kelp patches, not the edge). Light was expressed as the percentage of above canopy light transmitted to the subcanopy (using a LI-COR LI-193 spherical senser and logger). Flow was measured using plaster clod cards and expressed as the percentage of above canopy flow. Sediment deposition was the proportion of above canopy deposition that was measured at the benthos using sediment traps, and sediment accumulation was the depth of the algal-sediment matrix on the reef surface measured with a ruler. The percentage cover of turf algae was determined by analysing photo quadrats of the surface of each reef. Density of MPB algae on the settlement slides was also used as predictor of mussel recruitment to RFHs. Broadly speaking subcanopy light, water flow, sediment accumulation, and turf algae cover decreased with kelp patch size and density, and there was a simultaneous increase in the amount of sediment deposition (Layton et al. 2019b; Shelamoff et al. 2019b).
Analyses and statistics
The effect of patch size (fixed effect covariate) and kelp density (fixed factor) on the number of MPB algal cells that recruited to the settlement slides was assessed using an analysis of covariance (ANCOVA) conducted in R (ver. 3.2.4). ANCOVA was also used to assess the recruitment of mussels to RFHs and P:B, with the additional fixed factor of habitat position (reef edge vs. centre). Model assumptions were checked using diagnostic plots (for normality, linearity and homoscedascity) and model residuals (for linearity and homoscedascity). Data transformations were based on the maximum λ coefficient from the log-likelihood plots produced using the Box-Cox procedure. The covariate patch size was log2 transformed to linearise the data (reflecting that patch size increased on a log2 scale). Homogeneity of slopes was assessed by fitting the full model including the interaction term, and when the interaction term was non-significant (p > 0.25), the unsaturated model without the interaction term was fitted. Significant kelp density effects (p < 0.05) were further examined through pair-wise comparisons of covariate adjusted means with a Bonferroni adjustment of significance (to correct for multiple testing) (Quinn and Keough 2002). The potential influence of environmental correlates (light, flow, sediment accumulation, sediment deposition, and turf algae cover) on the abundance of MPB algal cells and mussels was assessed using multiple regression with the density of MPB algal cells also included as a predictor variable in assessing mussel recruitment. Normality, linearity and homogeneity of variance of the response variables was investigated through a scatterplot matrix and diagnostic plots (as outlined for ANCOVA). Multicollinearity of the predictors was satisfied through assessments of pairwise correlations and the variance inflation factor. Model selection was achieved by comparing the fit of all possible models for the two response variables (recruitment of MPB algae and recruitment of mussels) (Quinn and Keough 2002) using BIC (Bayesian information criterion) in the leaps package in R. We then used hierarchical partitioning to determine the independent contribution of all the predictor variables using the hier.part function. This allowed us to consider the relative independent effect of each of the predictors on the observed levels of recruitment. We then determined the likelihood that the independent contribution could be due to chance by performing a randomisation test and the assessing the significance of the Z scores to 95% confidence level.
Results
MPB algae
Overall, the benthic diatom Amphora spp. was the most abundant MPB organism recruiting beneath Ecklonia radiata canopies (68% of MPB cells) while other diatoms such as Cylindrotheca sp., Melosira sp., and Navicula sp and the dinoflagellate Gynmodinium sp. settled at much lower densities (< 1%) and only beneath lower-kelp-density treatments. There was an interactive effect of kelp density and patch size on the recruitment of MPB algae (Fig. 2, Table1), reflecting that decline in MPB cell density with increasing reef size was only evident on reefs supporting medium to high kelp density. Omitting both the zero-density and the low-density kelp treatments indicated no significant differences in MPB cell density across medium- and high-density kelp treatments.
Mussels
Recruitment
There was a significant interactive effect of patch size, kelp density, and habitat position on the recruitment of mussels to rope fibre habitats (RFHs) (Fig. 3a, b; Table 1). This reflects that declines in mussel density with increasing patch size was evident only on reefs supporting kelp cover, and in RFHs located in the centre of reef (i.e., not at the edge of the patch). There was no effect of patch size on the recruitment of mussels to RFHs located at the edge of reefs, however, recruitment to these edge positions was significantly elevated on reefs without kelp compared to reefs with kelp at the medium/natural density.
P:B
Productivity to biomass ratio (P:B) of mussels was similar in RFHs located in both edge-of-patch and central position, and increased significantly with patch size (Fig. 3c, d; Table 1). Kelp density significantly affected P:B in the two-way ANCOVA although there were no significant differences in pairwise comparisons. Kelp density was not significant in either of the separate one-way ANCOVAs for each habitat position.
Environmental covariates
The multiple regression indicated that light alone was the main factor correlated with the density of MPB algal cells, explaining around half of the variability (adjusted r2 = 0.47, BIC = − 12). The more complex model including light and turf algae cover had the same explanatory power as the simpler model, but a higher BIC (adjusted r2 = 0.47, BIC = − 9.8) (Fig. 4). Subsequent hierarchical partitioning, however, supported the more complex model, showing that turf cover (24%) and light (44%) both had significant independent effects on MPB algae recruitment, whilst sediment accumulation (13%), water flow (3%), and sediment deposition (16%) had non-significant independent effects. The optimal multiple regression models explaining variability in the abundance of mussel recruits to centre RFHs in terms of the adjusted R2 value (0.76) all included turf algae cover, light, sediment accumulation, and sediment deposition, with water flow and MPB algae additionally being included in some of those models (Fig. 5). However, two simpler models, one including just turf algae cover, light, and sediment accumulation, and the other including just turf algae cover, sediment accumulation, and sediment deposition had the lowest BIC value (−28) albeit with slightly lower adjusted R2 values (0.75 and 0.74, respectively). Subsequent hierarchical partitioning supported the simpler model with turf algae cover (28%), light (21%) and sediment accumulation (24%) being the only factors having significant independent effects on mussel recruitment, whilst water flow (7%), sediment deposition (12%) and MPB algae (8%) had non-significant independent effects.
Discussion
Increasing kelp cover in terms of both patch size and kelp density negatively affected the recruitment of microphytobenthic (MPB) algae and mussels. Densities of both declined with increasing patch size except on reefs where kelp was absent and for mussels on rope fibre habitats (RFHs) located at the reef edge. The adverse effect of kelp patch size on mussels also manifested in higher productivity:biomass (P:B) on larger reefs, indicating relatively few mussels reached the larger size classes on the larger reefs.
Low light and low cover of turf algae were identified as potential drivers limiting the abundance of MPB algae beneath kelp canopies, whilst low light, low turf cover, and low sediment accumulation appear to be drivers limiting mussel recruitment in the centre of reefs. Our results demonstrate that ecosystem engineers can generate an undesirable or hostile environment for other species to recruit into. Recruitment inhibition of competitors provides a mechanism by which ecosystem engineers can support their own persistence. Contrary to the notion that reefs devoid of kelp and covered in turf algae present a harsh physical and chemical environment that adversely affects associated species (Filbee-Dexter and Wernberg 2018; Layton et al. 2019a), these types of reefs supported high establishment and persistence of MPB algae and mussels (Figs. 2, 3; Table 1), indicating that these ecosystems may yet support high productivity of certain species.
MPB algae
This adverse effect of E. radiata on MPB algae is in stark contrast to the beneficial effect intertidal macroalgae have on MPB settlement in providing shelter and helping to concentrate nutrients for benthic diatoms with low irradiance requirements (Sundbäck and McGlathery 2005; Umanzor et al. 2018). Consistent with our prediction, the negative effect of shading appears to overcome any positive engineering effects from the kelp, presumably by limiting photosynthesis of MPB cells that typically have a relatively high light optimum (Blanchard and Montagna 1992). Our results also suggest that turf algae which thrived on reefs with limited kelp cover, provided a beneficial environment for MPB algae. This beneficial effect may result from the high surface area of filamentous turf algae and/or the large amounts of entrapped sediments providing additional habitat. In contrast, larger foliose and corticated macroalgal species, which tended to dominate beneath a fuller canopy of E. radiata on our reefs (Shelamoff et al. 2019b), have a relatively low surface area, less associated sediments, and produce a secondary canopy which further limits light transmission to the benthos (Tait and Schiel 2018). The highly variable effect of the low-density kelp treatment on MPB algal recruitment probably resulted from the specific placement of the settlement slides with respect to the patchy canopy cover (i.e., placement directly beneath the canopy resulted in low recruitment, whilst placement beneath a gap in the canopy resulted in high recruitment). Finally, MPB algae did not appear to influence the settlement of mussels, although other MPB organisms (e.g., bacteria, cyanobacteria), have been shown to enhance settlement of mussels and other marine organisms in studies elsewhere (Wang et al. 2012; Yang et al. 2017; Dobretsov and Rittschof 2020).
Reductions in the productivity of MPB algae through canopy shading could constrain levels of secondary productivity of epifaunal invertebrates (which often feed on the MPB algae) on kelp dominated reefs compared to turf-dominated reefs (Fraser et al. 2020; Shelamoff et al. 2020a). As these epifauna are a major food source for higher trophic level consumers, their diminished productivity could reduce the overall productivity of the associated food web. However, kelp also facilitate the export and deposition of resource subsides through the erosion of their tissue, through increasing localised concentrations of holoplankton, and by elevating the deposition of detritus (Duggins et al. 1989; Miller and Page 2012). It is possible that these effects could counteract any reduction in food-web productivity triggered by canopy shading of the benthos. Nevertheless, it is clear that the productivity of benthic MPB algae is likely to increase in response to kelp loss and fragmentation and decrease in response to kelp forest restoration.
Mussels
The observed negative effect of E. radiata on mussel recruitment is consistent with some other studies on subtidal bivalves (Witman 1987; Kochmann and Crowe 2014), but contrary to others where kelp has been shown to manifest a beneficial effect (Bulleri et al. 2006; Shelamoff et al. 2019a). In contrast to expectation and findings by Duggins et al. (1990) and Yang et al. (2017), our results do not support the notion that high water flow or MPB algal growth had major positive influences on mussel recruitment. Our results suggest M. galloprovincialis larvae are positively phototactic during settlement; however, this is in contrast to the negatively phototactic behaviour of M. edulis during settlement (Bayne 1964). The observed positive association with light is, however, consistent with observed reductions in recruitment of M. galloprovincialis with depth if driven by diminishing light levels with depth (Curiel-Ramirez and Caceres-Martinez 2010). This contrasts an increase in M. galloprovincialis recruitment with depth reported over a shallower depth range (Aghzar et al. 2012). We suspect that this apparent contradictory effect of depth may be explained by the positive influence of light on recruitment being outweighed by other physical factors such as increased stress from water motion at shallower depths, whilst these additional factors diminish in importance with depth.
Turf algae cover may have positively influenced mussel recruitment through certain algal species providing positive chemical cues for settlement, through the fine algal filaments providing a beneficial surface for attachment, or through their suppression of other foliose or leathery algal species which could have negative effects on mussel recruitment (Eyster and Pechenik 1988; Dobretsov 1999). It is unclear why there was a positive correlation between the depth of sediments within the algal-sediment matrix on our reefs and the recruitment of mussels to RFHs. Potentially, this could be a result of the algal-sediment matrix providing additional settlement cues which attract settling larvae, or this matrix may have resulted in increased food availability for mussels through greater retention and resuspension of food particles close to the reef surface. Additionally, it is also possible that the E. radiata canopy provided a physical barrier to settling larvae or it may have provided negative settlement cues. Once settled, canopy sweeping and scouring of the benthos may have also dislodged vulnerable newly settled individuals (Connell 2003).
Abiotic engineering of water flow and scour by E. radiata may have also contributed to the high P:B of mussel recruits (through many of the same mechanisms described above) on the larger reefs with kelp. However, high P:B values may additionally result from size selective predation (Edgar and Aoki 1993). On our experimental reefs, the abundance of invertivorous fishes increased with kelp cover (Shelamoff et al. 2020b), and these fishes may have targeted larger sized prey invertebrates (i.e., mussels recruits). Although predation can affect the size structure of invertebrates and therefore P:B, predators do not necessarily affect the choice of initial settlement location for mussels (von der Meden et al. 2015). Turf algae which dominated in the absence of kelp, appeared to have a positive effect on recruiting mussels by increasing numbers and decreasing P:B. Whilst turfs are likely to negatively impact the recruitment of the native oyster Ostrea angasi (Shelamoff et al. 2019a; McAfee et al. 2020), filamentous turf algae could act as a conduit for mussel settlement (Yang et al. 2007; Fitzsimons et al. 2019).
The beneficial effects of small patch size, reef edges, and absence of kelp on mussel recruitment suggests that increasing fragmentation of kelp habitat is likely to provide more attractive and favourable conditions for M. galloprovincialis recruitment and establishment. It is also possible that the replacement of kelp forests by turf algae could positively affect mussels. Clearly then, restoration of M. galloprovincialis shellfish reefs is unlikely to be enhanced through a multi-species approach involving canopy-forming kelp (unlike the case for O. angasi). An important caveat in interpreting these results and results from other studies that focus only on short-term recruitment of shellfish, is that initial recruitment patterns may not necessarily reflect long-term population establishment (Azpeitia et al. 2019) and that mussel populations are not necessarily stable over longer time periods (Ardizzone et al. 1996). At the end of the 2-year deployment of our reefs it was apparent that large-sized mussels (beyond the size of the recruits on the RFHs) were densely aggregated on the reef underside and to a lesser extent in the holes of the pavers. Potentially, strong competition with O. angasi on the upper surface of the reefs and increased refuge in the crevices influenced this distribution. These types of microhabitats may be required to support the longer-term persistence of mussels.
While canopy-forming kelp clearly have a facilitatory role in supporting the establishment and maintenance of species rich assemblages in some instances, these ecosystem engineers are also able to maintain dominance through creating an undesirable habitat for establishment of some species, thereby supressing potential competitors (Falkenberg et al. 2012; Miller et al. 2018). Our study suggests that the ability of ecosystem engineers to facilitate or supress other associated species can clearly depend on their patch size and their density.
Data availability
The full dataset associated with this research are available from IMAS Data Catalogue (Shelamoff et al. 2022).
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Acknowledgements
This study was funded by an Australian Research Council Discovery Grant DP130101113; (www.arc.gov.au) awarded to JTW and CRJ. This study would not have possible without generous assistance in the field and laboratory provided by many volunteers but especially Claire Butler and Kathy Willis. We would also like to thank the reviewers.
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Open Access funding enabled and organized by CAUL and its Member Institutions. This study was funded by an Australian Research Council Discovery Grant no DP130101113; (www.arc.gov.au) awarded to JTW and CRJ.
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VS, SU, CL, MT, MJC, JTW, CRJ conceived, designed, and performed the experiments; VS analysed the data and wrote the manuscript; SU, CL, JTW, CRJ edited the manuscript. We confirm this manuscript and data are original and have not been previously published or considered elsewhere.
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Shelamoff, V., Umanzor, S., Layton, C. et al. Ecosystem engineering kelp limits recruitment of mussels and microphytobenthic algae. Mar Biol 169, 85 (2022). https://doi.org/10.1007/s00227-022-04072-5
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DOI: https://doi.org/10.1007/s00227-022-04072-5