Abstract
Marine heatwaves (MHWs) are projected to increase in intensity and frequency over the coming decades, and it is imperative to assess the adaptive capacity of marine organisms to these extreme temperature events. Given the nature of MHWs to last days to weeks in a region, these events may have overarching impacts on phenological events like reproduction and development. Here, the role of adult thermal history and transgenerational plasticity may be an important pathway by which MHWs are transduced to impact community structure. In this study, we sought to explore the effects of paternal thermal history in the purple urchin, Strongylocentrotus purpuratus, on a crucial aspect of reproduction, fertilization. Using ecologically relevant temperatures representative of both MHW events that occurred in 2014–2020 and non-MHW temperatures in our region of the California Large Marine Ecosystem, we conditioned male S. purpuratus for 28 days to either a high, MHW or a low, non-MHW temperature. Following the temperature acclimation of adults, sperm performance was tested for individual males by conducting fertilization success trials at varying temperatures and sperm concentrations. While sperm appeared robust to elevated temperature during fertilization, sperm produced by high-temperature-acclimated males had overall diminished performance as compared to those acclimated to non-MHW temperatures. These results suggest MHW events will have a negative impact on fertilization in situ for S. purpuratus populations. Furthermore, these results highlight the importance of considering both male and female environmental history in projections of reproduction under climate change scenarios.
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Introduction
Understanding the adaptive capacity of species in response to climate change is a major research and conservation goal (Ofori et al. 2017; Thurman et al. 2020). In marine systems, for organisms not as reliant on migration and dispersal in response to unfavorable climatic conditions, adaptive capacity can be thought of as having two elements: micro-evolutionary processes (where genetic changes occur in response to selection in the environment) and phenotypic plasticity (where species change their physiology and morphology without alteration to the underlying genome) (Hoffmann and Sgro 2011; Bay et al. 2018). Since phenotypic plasticity may act more rapidly, and on ecological time scales, this class of mechanisms might allow species to cope with environmental change in situ (Fox et al. 2019). In this study, we explore gamete plasticity and its effects on the fertilization process in the context of extreme heat stress that occurs during episodic marine heatwaves (MHWs), asking if paternal experience contributes to the generation of gametes more resistant to the thermal conditions of MHWs.
Marine heatwaves, represented by discrete periods of anomalously warm temperatures (compared to an area’s historical baseline) lasting longer than 5 days (Hobday et al. 2016; Oliver et al. 2021), have increased in frequency in the last decade and estimates are that these anomalous heat events will occur with greater frequency in future global oceans (Frolicher et al. 2018; Oliver et al. 2018; Smale et al. 2019). Recent analysis by the IPCC and others predict that these events will become a decadal certainty in the high (RCP8.5) greenhouse gas emissions scenario, with MHW events by the end of century projected to occur every two days, last 126–152 days, and reach maximum temperatures 3.1 °C–3.8 °C higher than the 99th percentile of temperatures from an area’s historical baseline (IPCC 2019). Ecologically, MHW events threaten the biodiversity of marine coastal habitats through disruptions to ecosystem structure and function (Harris et al. 2018; Smale et al. 2019). On shorter timescales, intense thermal stress associated with these events can push organisms past their physiological limits, leading to massive mortality events (Wernberg et al. 2016; Harvell et al. 2019; Seuront et al. 2019). Even populations that can survive the initial temperature stress can manifest the effects of MHWs years later through altered reproductive and recruitment success (Pineda et al. 2018; Shanks et al. 2020), loss of habitat-forming species (Marba and Duarte 2010; Rogers-Bennett and Catton 2019), or the ecological consequences associated with poleward shifts of species into or out of parts of their biogeographic distributions (Sanford et al. 2019). These impacts on coastal marine ecosystems coupled with forecasts of more MHWs in the future together underscore the pressing need for more research into the biological consequences of MHWs, including whether specific populations have the adaptive capacity to withstand these short-term, extreme climatic events. Additionally, the formation of MHWs and their intensity is correlated to El Niño years, leading to regionally-specific temporal patterns in when these events are seen most frequently (i.e. from summer to early Fall along the west coast of North America) (Sen Gupta et al. 2020). Such seasonality must be considered when projecting what organismal functions and life history stages may be most impacted by future MHW events.
In coastal marine ecosystems along the Pacific coasts of North America, reproduction of benthic invertebrates varies but can be highly seasonal (Strathmann 1987). For example, many sea urchin species consistently undergo gametogenesis in the early fall and undergo spawning events the following winter (Cochran and Engelmann 1975). In addition, the response of marine taxa to environmental stressors, such as elevated temperature, varies with ontogeny, highlighting the need to consider life history in projections of a species’ adaptive capacity (Przeslawski et al. 2015; Thurman et al. 2020). For species with complex life history strategies, as is the case for many marine invertebrates, early life history stages appear to be the most sensitive to the environmental stressors associated with ocean warming, ocean acidification, and hypoxia (Byrne 2011; Pandori and Sorte 2019). Extreme ocean warming experienced by these early stages has been documented to increase mortality, decrease growth, as well as promote stalled and abnormal development (Rahman et al. 2009; Randall and Szmant 2009; Byrne 2011; Martino et al. 2021). As many of these species are broadcast spawners that release their gametes directly into the water column to facilitate fertilization, their gametes also have the potential to be exposed to harsh conditions.
In contrast to the sensitivity of post-fertilization life stages, the general effect of increased temperature on gametes and the resulting fertilization is more contested. Fertilization can occur with unaltered success under moderate increases in temperature for many species (Byrne et al. 2010a; Enricuso et al. 2019; Pitts et al. 2020), with several studies observing positive effects of elevated temperature on echinoderm and mollusk fertilization (Ho et al. 2013; Foo et al. 2014; Eads et al. 2016b). Alternatively, a handful of studies document decreased fertility under high temperature (Parker et al. 2010; Albright and Mason 2013), with these conflicting results even observed within the same species challenged by similar temperatures (Byrne et al. 2009; Eads et al. 2016a; Chirgwin et al. 2020). These inconsistencies highlight the variability associated with measuring fertilization success as a result of differences in methods, parental crossing designs, distances between experimental temperatures from the study organism’s thermal limits, and parental environmental histories (Byrne 2011). Given the importance of fertilization for both the success of individuals and populations, it is imperative to gain a better understanding of the mechanisms through which environmental stressors, like temperature, affect the fertilization process.
At the individual gamete-level, the morphology and physiology of both eggs and sperm influence fertilization success. For example, larger eggs provide a bigger target for increased fertilization (Levitan 2006) while sperm phenotype and motility can impact fertilization kinetics (Marshall 2015). While these factors can play a role in the success of fertilization under stressful environments, the phenotypes themselves may also be influenced by the environmental conditions experienced by the parents. Altered maternal provisioning to eggs is well documented in response to temperature, impacting egg size and content (Steer et al. 2004; Hamdoun and Epel 2007; Suckling et al. 2015). Alternatively, there is limited strong evidence across taxa that male thermal history can alter sperm quality and performance (Crean and Bonduriansky 2014; Boni et al. 2016; Immler 2018). In the tubeworm species, Galeolaria caespitosa, fertilization success was more influenced by paternal temperature environment than the temperature experienced during maternal conditioning, with significant decreases in fertilization (− 30%) seen in males exposed to + 6 °C for 14 days (Guillaume et al. 2016). While a few studies have tested other paternal environment factors on fertilization (Crean et al. 2013; Jensen et al. 2014), this is the only study to date testing the influence of temperature. Given the short timescales in which paternal effects can be seen, extreme climatic events like MHWs could disrupt the reproductive potential of populations through sire-related impacts on fertilization and development.
To explore the impact of temperatures observed in MHW events on fertilization, we chose to study the ecologically and economically relevant purple sea urchin, Strongylocentrotus purpuratus (Pearse 2006). With a broad biogeographic distribution across the northeastern Pacific coast of North America, S. purpuratus populations are exposed to dynamic oceanic conditions associated with the temperature and pH mosaic observed within the California Current Large Marine Ecosystem (CCLME) (Chan et al. 2017). Ecologically, purple urchins are vital members of the kelp forest communities across the CCLME (Ebert 2010; Yorke et al. 2019). As grazers of kelp recruits, urchin density influences the shift of their habitat from a rich, kelp forest to a kelp-depleted “barren” and vice versa (Dayton et al. 1992). This role in shaping kelp forest communities has made monitoring changes in S. purpuratus populations a key element of studies assessing the health of kelp forests following MHW events (Reed et al. 2016; Rogers-Bennett and Catton 2019; Okamoto et al. 2020).
In general, the effect of temperature on urchin fertilization has been studied extensively (Rupp 1973; Sewell and Young 1999; Byrne et al. 2010a; Ericson et al. 2012; Foo et al. 2014; Mak and Chan 2018). While S. purpuratus served as the model for early work on general fertilization there are only a few studies exploring these dynamics in climate change scenarios. In these earlier studies, S. purpuratus fertilization success was independently sensitive to extremely high-temperature (+ 10 °C) and low pH conditions (Farmanfarmaian and Giese 1963; Kapsenberg et al. 2017). Given the environmental reality of future exposure to MHW conditions, in this study, we sought not only to explore the effects of elevated temperature on fertilization directly but also how the process was influenced by paternal heat exposure. Here, we acclimated groups of male adult urchins for 28 days at 2 temperature conditions—a non-MHW treatment (14 °C) and a MHW treatment (20 °C). Treatment temperatures were chosen based upon temperature data collected from 2014—2020 within the Santa Barbara Channel (using the SBC LTER database: https://sbclter.msi.ucsb.edu/data/catalog/), where multiple MHW events were observed (see also Fig. 4 within Discussion section). Here, we report on the effects of MHW temperatures on aspects of fertilization biology and early life history events in S. purpuratus: (1) sperm performance via fertilization success trials, and (2) successful development of early stage embryos.
Materials and methods
Animal collection
Urchins were hand-collected on SCUBA in December 2019 from two separate temperate reef study sites in the Santa Barbara Channel; these locations are also Santa Barbara Coastal Long-Term Ecological Research (LTER; https://sbclter.msi.ucsb.edu/) sites: Mohawk Reef (34.394°N, 119.730°W) and Arroyo Quemado Reef (34.468°N, 120.119°W). Urchins in these locations, which are ~ 37 km apart, generally experience similar water temperature conditions and there is no evidence to suggest a difference in the timing of gravidity or reproduction between locations. Collections were conducted within a week of one another using California Scientific Collection permit SC-1223 (to G. Hofmann). Urchins from Mohawk Reef and Arroyo Quemado Reef were collected at depths of 8 ft. and 10 ft., respectively. Adult urchins were transported to the Marine Science Institute’s seawater research facility and placed in flow-through seawater aquaria held at 13 °C. Urchins from each location were kept separate and their location identity was tracked for the entirety of the study.
Determination of urchin sex
In the days following collection, urchins were sexed by a physical induction of spawning via one minute of shaking outside of water. From this process, 74 urchins (representing ~ 67% of the total urchins attempted to be spawned) were successfully identified as either male (n = 31 urchins) or female (n = 43 urchins) and separated into appropriately labeled tanks. All urchins were given a recovery period at ambient temperatures while being fed kelp (Macrocystis pyrifera) ad libitum for 2–3 weeks following sexing. Any individual urchin that exhibited signs of significant injury, disease, or death during this period was promptly removed and not included in the experiment. Over the time spanning both recovery and the subsequent experimental acclimation, only 10 out of the original 111 urchins exposed to this spawning induction method were removed for signs of death or disease, indicating that this procedure for urchin sex identification did not significantly influence experimental results.
Acclimation of adult sea urchins
After the recovery period, male urchins were acclimated to either a high (20 °C) or low (14 °C) temperature treatment for 28 days (Fig. 1). The 28-day acclimation period was used to both reflect a timeframe that is within the duration of past MHW events as well as overlap with the production of new sperm cells within the regularly fed male sea urchins (personal communication). This would increase the likelihood that the sperm used in this study included those produced under experimental conditions mimicking either ambient or ecologically relevant heat stress conditions. Male urchins were randomly selected and placed in one of the three acclimation tanks in either the high temperature (H) or low-temperature treatment (L). Test diameters for each urchin were measured to ensure that no significant size difference existed between tanks or treatments. Acclimation tanks consisted of a 10-gallon tank equipped with an aquarium pump (Aqua-Supreme) and supplied with UV-sterilized, filtered seawater at a rate of 12 L/hr through irrigation button drippers (DIG Corporation). Tanks from each treatment were half-way submerged in a large water bath where temperatures could be controlled. Seawater temperature was controlled using a Delta Star heat pump with a Nema 4 × digital temperature controller (AquaLogic). Temperatures in each tank were monitored through point measurements with a wire thermocouple (Thermolyne PM 20,700 / Series 1218) and for the entirety of the acclimation, every 5 min, by Hobo temperature loggers (Onset Computer Corporation). Once per week, each acclimation tank received one kelp blade per urchin present. Each weekly feeding interval lasted three days before any uneaten kelp material was removed. Female urchins were kept in multiple 10-gallon flow-through tanks maintained at ambient temperatures, approximately 13 °C and fed kelp ad libitum for the entirety of the male acclimation.
Due to the limited resources and time available for this study, adult acclimations and the following spawning were conducted in three blocks staggered by 96 h from one another. To facilitate these staggered adult acclimations, each male acclimation tank was separated into three subdivisions representing each of the three blocks. For clarity, the first block of adult acclimations involved the random selection of 12 male urchins from the recovery tanks that were then placed into one subdivided section of 6 different tanks (n = 2 urchins per tank section), 3 replicate high-temperature acclimation tanks and 3 replicate low-temperature tanks. After 96 h, the second block was initiated by selecting 12 more male urchins and placing them into a separate, subdivided section for each of the same 6 acclimation tanks previously mentioned. This process was repeated one last time 96 h after that for the third block of this experiment. Temperatures, pCO2 levels, and urchin behavior were closely monitored throughout to ensure that there were no significant disturbances to the urchins already present in the acclimation tanks when urchins were added or removed. This method was implemented to allow increased replication while ensuring that each block’s male urchins endured exactly 28 days of acclimation before spawning without a significant amount of time between spawnings.
Urchin spawning and experimental design
During each block, male and female urchins were randomly selected to be spawned. Males from different replicate acclimation tanks were represented within each block. This time, urchin spawning was induced through a combination of intercoelomic injections of 0.55 M KCl and physical perturbation. Eggs were collected by inverting females over beakers filled with ~ 15 °C filtered seawater (FSW) and sperm was collected dry and kept on ice (Strathmann 1987). Gamete quantity and quality were observed under a light microscope. Gamete compatibility was assessed by conducting test fertilizations using every combination of males and females. During these test fertilizations, 15 uL of concentrated eggs was added to 3 mL of FSW then oversaturated with sperm. Male urchins demonstrating healthy sperm (e.g. good motility and > 95% of eggs fertilized) were used to further assay fertilization success. Sperm was used within 6 h of collection.
Each block utilized a modified split-clutch crossing design for measuring fertilization success: involving the fertilization of pooled eggs (n = 6 females) by three males from each high and low treatments, resulting in 6 unique crosses. This crossing design with pooled eggs was utilized to minimize the effects of male–female interactions on fertilization and subsequent development. Across the three blocks, 9 males from each treatment and 3 pools of females were used to proxy this question over a total of 18 crosses.
Protocol for fertilization success trials
The consequences of paternal heat exposure were assessed by performing fertilization trials under two different fertilization temperatures across three sperm concentrations (Fig. 1). Trials were conducted following protocols outlined in Kapsenberg et al. (2017) and Crean et al. (2013). First, samples of each male’s sperm were concentrated via centrifugation and excess fluid was removed, leading to a more standardized measure of sperm concentration across all individuals. Concentrated sperm from each male (n = 3 males per treatment per block) were diluted 1:200 with FSW to create sperm stock solutions. Actual sperm concentrations were calculated post trial by preserving a subsample of each sperm stock in 2% formaldehyde–seawater and counting sperm cells using a hemocytometer (Hausser Scientific) (Sewell et al. 2014). From these stocks, serial dilutions were performed in triplicate 25-mL glass vials to create 20-mL solutions of three varying sperm concentrations (1:10, 1:100, or 1:1,000) for both fertilization temperature treatments. Depending on the treatment, sperm was diluted at this point using either H or L treated FSW. Temperatures within vials were maintained throughout the assay using a temperature-controlled aluminum block (for H treatment: 20 °C) or a cold room (for L treatment: 15 °C) and measured via a thermocouple (Table 1). Target sperm concentrations were based off those used in past assays of S. purpuratus fertilization success (Kapsenberg et al. 2017), but targeting specific concentrations that led to < 100% fertilization under ambient conditions. Previous research suggests the use of lower sperm concentrations to more accurately assess the effect of environmental stressors on the fertilization process (Marshall 2006; Jensen et al. 2014; Guillaume et al. 2016). This justification applied specifically to the lower two sperm concentrations, but a third, higher sperm concentration (1:1000) was also included during the trials to both: (1) ensure that under the given conditions, sperm from each of our males would be able to fertilize all the eggs, and (2) provide potential information about the effect of MHW temperatures on the susceptibility of eggs to polyspermy. Vials of diluted sperm were homogenously resuspended by inversion before the addition of eggs.
For the trials, 1,000 eggs (suspended in 2 mL FSW) were added to each scintillation vial, bringing the total volume in each vial to 22 mL (~ 45 eggs/mL). For every trial, eggs were added to vials within 5 min of sperm activation for each male. Egg-sperm contact time was limited to 30 s after egg addition, with further fertilization events inhibited by adding 1 mL of 0.55 M KCl to each vial (Farley and Levitan 2001). A control vial, containing no sperm before egg addition, was run in conjunction with each trial’s experimental vials to ensure that there was no sperm contamination or spontaneous fertilization envelope (FE) elevation during the trial that could skew results. Control vials containing even a single fertilized egg would invalidate that trial, leading it to be thrown out before any analysis was conducted. Once fertilization was inhibited, vials of fertilized eggs were kept at the treatment temperature (H or L) for around 5 min before they were moved to a 14 °C cold room and allowed to develop for at least 2 h before scoring for fertilization success (Table 2). Fertilization success was assayed for > 75 eggs per vial. Successful development was scored by the presence of a smooth, elevated FE and equal, holoblastic cleavage. The number of unfertilized eggs and abnormal embryos (tight fertilization membranes or delayed/abnormal cleavage) were also scored (Sewell et al. 2014; Kapsenberg et al. 2017). These classifications were later used to assess two sides of the fertilization process: (1) fertilization events, or how well sperm was able to find and fuse with egg under the 30 s time limit, and (2) development, or how the subsequent cleavage proceeded after fertilization. The proportion of successful fertilization events was calculated by the following equation:
The proportion of normally and abnormally developed embryos were then calculated from the eggs that were fertilized:
Out of the 36 fertilization trials conducted for this experiment, only one revealed the presence of fertilization in the control. This trial (testing an H male’s fertilization success at the L fertilization environment) was thrown out and not considered during data analysis.
Statistical analysis
The influence of paternal temperature on the proportion of fertilization events observed was analyzed as a binomial response with a generalized linear mixed-effects model. The binary data (fertilized vs unfertilized) were fitted using a logit link function. The model included paternal temperature treatment, fertilization temperature treatment, and sperm concentration as fixed effects in addition to both experimental round and male identity as random effects. Non-significant random terms, such as site location and some of the interactions between fixed factors, were removed from the analysis following standard model reduction procedures outlined in Quinn and Keough 2002. A Wald chi-square test was employed to further determine the significance of each factor on the number of fertilization events observed. To determine if the sperm concentrations referenced in the model were themselves influenced by paternal temperature acclimation, the relationship between paternal temperature group and sperm concentration was examined using a Welch two-sample t test. Additionally, the relationship between fertilization success and the sperm concentrations corresponding to dilutions (1:10, 1:100, 1:1000) for each individual male were analyzed by linear regression and found no significant correlation (R2 < 0.1), so concentrations were binned into three categories (HIGH for 1:10, MID for 1:100, LOW for 1:1000) for use in the previously described model.
Analysis of how paternal temperature affected the proportion of fertilized eggs that developed normally was also analyzed with a generalized linear mixed-effects model. This dataset, again modeled as a binomial response fitted with the logit link function, excluded data points corresponding to eggs fertilized under the LOW sperm concentration as all replicates had < 10 eggs that had even been fertilized to start with. As such, sperm concentration, now containing only two categories (MID and HIGH), was modeled as a fixed effect in addition to paternal temperature treatment and fertilization temperature treatment. Male identity and round were included as random effects once again. Again, non-significant random terms were removed following standard model reduction procedures and a Wald chi-square test was utilized to determine whether each factor had a significant effect on normal development (Quinn and Keough 2002). All statistical analysis was performed in R (version 3.6.3) with models created using the lmer4 package (Bates et al. 2015).
Results
Fertilization events
In general, male thermal history significantly impacted the number of successful fertilization events observed in vitro at all sperm concentrations (Table 3; p = 0.035). In addition, both sperm concentration alone, and the interaction between sperm concentration and the temperature at which fertilization occurred, had significant effects in the model (Table 3). Within each sperm concentration, a lower proportion of eggs were fertilized by sperm from high-temperature males (HIGH: 97.2 ± 0.7%, MID: 44.3 ± 2.4%, LOW: 6.2 ± 0.6%, presented as mean ± standard error) as compared to sperm from low-temperature males (HIGH: 98.0 ± 0.7%, MID: 53.7 ± 3.1%, LOW: 9.2 ± 0.8%) (Fig. 2). The effect of paternal temperature was most pronounced under the middle (MID) sperm concentration, where high-temperature males produced sperm that resulted in ~ 9% less fertilization than that of low-temperature males. The other sperm concentrations exhibited a similar pattern but to a lesser degree, with high-temperature males having ~ 3% and ~ 1% less fertilization under the most dilute (LOW) and concentrated (HIGH) sperm conditions, respectively.
Not surprisingly, sperm concentration was a highly influential parameter in the experiment, displaying a positive relationship with the proportion of fertilized eggs observed (Table 3; p < 0.001). Measured sperm concentrations, calculated from a sample taken from each male’s original sperm stock solution, averaged 1.46 × 106 ± 7.32 × 104 sperm.mL−1. There was no observed difference between the sperm concentrations produced by differentially acclimated males (t (16) = 0.043, p = 0.9663), with sperm concentrations from high-temperature males averaging 1.48 × 106 ± 1.11 × 105 sperm.mL−1 compared to 1.44 × 106 ± 9.81 × 104 sperm.mL−1 in low-temperature males. Based on these calculations and estimations from conducted serial dilutions, we assume that the concentrations were roughly 105, 104, and 103 sperm.mL−1 for the HIGH, MID-, and LOW sperm concentration groups, respectively. The proportion of fertilized eggs increased positively with these estimated sperm concentrations (Fig. 2), with a majority of eggs remaining unfertilized in the most dilute sperm conditions (LOW: 7.7 ± 0.54%) to nearly all eggs exhibiting fertilization under the most concentrated sperm conditions (HIGH: 97.6 ± 0.49%). The MID-sperm concentration exhibited an almost even split between the fertilized and unfertilized eggs observed (48.9 ± 2.1%). These concentrations also exhibited an interactive effect with fertilization temperature (Table 3; p = 0.002). Note that there was no significant effect of fertilization temperature alone on the proportion of fertilized events observed (Table 3; p = 0.096).
Development of embryos
There was not a detectable effect of paternal thermal history on the proportion of fertilized eggs that cleaved normally (Table 4; p = 0.35). Alternatively, there were significant effects of sperm concentration and the interaction between sperm concentration and paternal thermal history (Table 4). As the trials associated with the LOW sperm concentration all yielded < 10 fertilized eggs, they were not included in this analysis due to low sample sizes. In comparing the normal development of fertilized eggs between the MID and HIGH sperm groups, we observed a large significant effect of sperm concentration (Table 4; p < 0.001). The proportion of normally developed fertilized eggs was diminished with higher sperm concentration, with only 32.5 ± 1.7% of fertilized eggs developing normally under high concentrations as opposed to 75.4 ± 1.9% for the middle concentrations (Fig. 3). While there was no significant effect of either paternal or fertilization temperature on the proportion of normal development (Table 4; p = 0.18), under the HIGH sperm concentration, embryos from high-temperature males that experienced high temperatures during fertilization exhibited more normal development (37.5 ± 2.9%) than any other combination of paternal and fertilization temperatures (HL: 31.3 ± 2.7%, LH: 28.7 ± 4.4%, LL: 31.4 ± 3.5%). This pattern was not observed under the MID-sperm concentration, driving the significant interactive effect of concentration and paternal temperature seen in the model (Table 4; p = 0.01).
Discussion
In this study, we examined the fertilization success of the purple sea urchin, Strongylocentrotus purpuratus, under the elevated temperatures associated with marine heatwave (MHW) scenarios. Our experimental goal was to determine how MHW-like temperatures experienced during spermatogenesis and fertilization would influence the fertilization process. To meet this goal, adult male sea urchins were exposed to two temperatures, 14 °C or 20 °C, for 28 days before their sperm was assessed via fertilization success trials under the same temperature treatments. In examining the role of paternal thermal history on fertilization dynamics under these temperatures, we discovered two central findings: (1) the temperature at which fertilization occurred had no significant effect on fertilization itself, and (2) paternal exposure to a MHW-like temperature had a negative impact on fertilization success as compared to the non-MHW treatment. Underlying the second finding is the observation that these patterns in fertilization success were driven mainly by a reduced ability of the sperm to reach the egg and fertilize it as opposed to abnormal development seen post-fertilization. Below we discuss each aspect of our study and relate our results to the phenology of the sea urchin life cycle relative to the timing of MHWs in our area.
Fertilization robust to high temperature
In our study, fertilization in S. purpuratus, regardless of paternal thermal history, was not significantly altered by high temperature. Successful fertilization under a high, MHW-like temperature (20 °C) was observed at similar proportions to fertilization occurring under a low, non-MHW temperature (15 °C). Although we did not directly measure any sperm traits in this study, a scoring design was utilized that allowed for more insight into what elements of the fertilization process could be most influenced by temperature. The presence of a fertilization membrane alone was used to calculate the proportion of fertilization events observed, which could serve as a proxy for general fertilization kinetics involved in the sperm reaching the egg and fertilizing it in a defined time frame. We then measured how many of those fertilized eggs demonstrated normal cleavage after 2 h at 14 °C, allowing for the assessment of abnormality caused by polyspermy, male–female incompatibility, or sperm DNA damage. Here, neither the proportion of fertilization events or normally cleaving embryos were significantly altered by fertilization temperature, but the effect was just non-significant (p = 0.095) for the number of fertilization events observed. Together, these results align with the prevailing view that fertilization in sea urchins displays a great deal of plasticity and is generally robust under ocean warming scenarios (Byrne 2011).
In terms of species comparisons, studies focused on how temperature affects sea urchin fertilization biology report some contrasting and species-dependent results. Most tropical and temperate sea urchins demonstrate successful fertilization over a wide range of temperatures, even under temperature challenges much higher than what the urchin populations experience in situ (Rupp 1973; Byrne et al. 2010b; Delorme and Sewell 2014; Foo et al. 2014). Several echinoid species across coastal Australia exhibit high fertilization success (> 80%) when challenged with temperatures 2–6 °C higher than that experienced locally (Byrne et al. 2010b; Foo et al. 2014). Alternatively, the fertilization of a few temperate and polar species exhibits a lower thermal tolerance threshold (Farmanfarmaian and Giese 1963; Byrne et al. 2009; Ericson et al. 2012; Gianguzza et al. 2014). Such vulnerabilities have renewed relevance when considering the anomalously high temperatures associated with current MHW events. For example, while the Mediterranean sea urchin species, Arbicula lixula, exhibited successful fertilization at temperatures above that predicted under near-future warming scenarios, the complete disruption of fertilization (< 1%) at temperatures 7 °C above ambient conditions leave A. lixula vulnerable to MHW events experienced in the area over the past decade (Olita et al. 2007; Gianguzza et al. 2014). Circumstances such as this are only becoming more common with increased MHW prevalence across global oceans (Oliver et al. 2018), potentially leaving species with narrow fertilization thermal tolerance windows, like the Antarctic sea urchin, Sterechinus neumayeri (Ericson et al. 2012; Ho et al. 2013), at risk sooner than projected under ocean warming scenarios.
This species-dependent thermal tolerance of fertilization is observed across other marine invertebrate taxa as well, including corals (Negri et al. 2007; Albright and Mason 2013) and mollusks (Parker et al. 2010; Armstrong et al. 2019; Enricuso et al. 2019). There is also evidence that a great deal of intra-species variability in fertilization success exists, with contrasting results observed in the mussel, Mytilus edilus (Eads et al. 2016a, 2016b), the sea urchin, Heliocidaris erthyogramma (Byrne et al. 2009, 2010a, 2010b), and the polychaete, Galeolaria caespitosa (Kupriyanova and Havenhand 2005; Chirgwin et al. 2020). This variation highlights the need to consider other factors, both biological (e.g. environmental history of study populations) and methodological (e.g. experimental design), that could influence fertilization dynamics under altered temperatures.
Methodological differences in fertilization success assays are common across studies, an issue prompting the creation of a more standardized approach for environmental monitoring (Lera et al. 2006). The type of crossing design used, for example, can heavily influence results, with the use of pooled gametes reducing the effects of male–female incompatibilities (Byrne et al. 2009; Eads et al. 2016b) and benefitting from the positive contributions of polyandry (Evans and Marshall 2005), while individual male–female pairings instead illuminate the extent of intra-individual variability within fertilization (Foo et al. 2014; Eads et al. 2016a). Our study employed the less-frequently used split-clutch design where sperm from individual males were used to fertilize eggs from the same pool of females (Crean et al. 2013). While some variability between males (within each paternal temperature treatment) did exist, this method appeared to conform to results seen in population-level approaches with no general effect of temperature on fertilization. Alternatively, fertilization success was significantly altered by the sperm concentration used, providing more evidence that these concentrations are an important factor to consider in the design of fertilization success assays (Marshall 2015). Here, the proportion of fertilization events increased positively with sperm concentration, most likely a result of more gamete collisions at higher ratios of sperm to egg (Kupriyanova and Havenhand 2002). The highest sperm concentration, estimated to be 105 sperm.mL−1, led to significant amounts of abnormality (~ 70%). This perceived polyspermy could have been induced by the combination of sperm concentration, contact time between gametes, and vessel size utilized in this study (Byrne 2011).
Studies aimed at uncovering the mechanistic basis of how temperature influences fertilization success could provide more standardized results in addition to accounting for individual variability. Research on fertilization under temperature stress attributes varied tolerance to differential maternal investment of heat shock factors to eggs (Yamada and Mihashi 1998) in addition to altered sperm kinetics and energy metabolism (Mita et al. 1984; Johnson and Yund 2004). For sperm specifically, elevated temperatures can increase the cell’s metabolic rate, visualized through diminished sperm longevity and increased respiration (Kupriyanova and Havenhand 2005; Rahman et al. 2009; Binet and Doyle 2013). As such, the inclusion of varied contact times between egg and sperm in addition to quantifications of sperm longevity, motility and respiration could provide useful insight for future studies quantifying fertilization under MHW scenarios.
Overall, the varying thermal tolerance of fertilization within and across marine taxa may support the idea that there is not one general effect of the environment on sperm cells but instead certain conditions that favor specific sperm phenotypes, affecting the fertilization success landscape (Marshall 2015). For example, the optimal head size of sperm cells of the tubeworm, Galeolaria gemineoa, was found to differ depending on the concentration and age of the sperm, with longer heads favored under both low concentrations and amongst aged sperm, as opposed to fertilization under high sperm concentrations where smaller heads (with longer tails) possessed increased success (Johnson et al. 2013). If this selection for specific sperm morphology holds true for other environmental conditions, the increased variation in sperm phenotypes associated with pooled sperm samples could heavily influence fertilization success results, especially when compared to studies using sperm from single males. Therefore, it is prudent for future studies of fertilization success to consider measures of sperm phenotypes in addition to what factors could influence the production of these specific phenotypes, such as paternal environmental history.
Paternal environment alters fertilization success
Based upon previous research on temperature effects in marine invertebrates (O'Connor et al. 2007; Zippay and Helmuth 2012), we hypothesized that paternal heat exposure would have a negative impact on fertilization success. Our results support this hypothesis as we observed diminished proportions of successfully fertilized eggs in male urchins exposed to 20 °C as opposed to 14 °C for 28 days. Similar, if not pronounced, results were seen in the tubeworm, Galeolaria caespitosa following a 2-week exposure of male and female worms to varied temperatures (Guillaume et al. 2016). For G. caespitosa, fertilization success in warm acclimated (21.5 °C) males was significantly lower than that of cool acclimated males (15.5 °C) across multiple sperm concentrations. To date, these are the only two studies that have explored the effect of paternal thermal acclimation on fertilization success in marine systems.
One pathway by which temperature could influence paternal function is via direct effects on gametogenesis. Generally, elevated temperatures can alter the gametogenesis process for both male and female organisms, but can be particularly detrimental to sperm production (Rogers-Bennett et al. 2010; Uthicke et al. 2014). Even when sperm are successfully produced under temperature stress, thermal history can be reflected in the sperm quality and performance (Boni et al. 2016; Johnstone et al. 2019). In this study, all male S. purpuratus were successfully spawned after a 28-day acclimation to 20 °C, but the sperm produced during this period had diminished performance regarding fertilization. Paternal exposure to high temperature exhibited a significant negative effect on the number of fertilization events observed across different sperm concentrations (LOW: -3%, MID: -9%, LOW: -1%). These patterns could potentially reflect compromised fertilization kinetics resulting from paternal temperature stress via alterations of sperm morphology, motility, or processes associated with sperm chemotaxis or acrosomal function (Immler 2018). Mussels exposed to 28 °C for a similar period of time (30 days) exhibited both decreased sperm motility and increased abnormality (Boni et al. 2016). Here, sperm from both paternal temperature treatments demonstrated nearly 100% fertilization at the highest sperm concentration, providing little evidence to suggest high-temperature males produced sperm that was unable to contact or fertilize eggs when given the opportunity (Marshall 2006). Environmental stress can additionally affect sperm quality in the form of DNA damage within the sperm cells themselves, leading to developmental abnormalities in embryos and larvae (Lewis and Galloway 2009; Johnstone et al. 2019). For S. purpuratus used in this study, measures of post-fertilization development (cleavage) varied only with sperm concentration, not with fertilization or paternal temperature alone.
While the presence of environmentally induced maternal effects has been acknowledged for years (Mousseau and Fox 1998), the potential for non-genetic paternal effects is just beginning to gain traction (Crean and Bonduriansky 2014; Marshall 2015). Depending on the predictability of the environment, adaptive paternal effects could exist where males under stressful conditions can confer tolerance to offspring experiencing similar environments (Marshall 2015). Positive impacts on larval survival, metamorphosis, and growth have been observed in response to perceived sperm competition (Crean et al. 2013) and salinity (Jensen et al. 2014), but not pH (Lane et al. 2015). Jensen et al. (2014) found that fertilization success in the tubeworm, Hydroides diramphus, was at its highest when the salinity during fertilization matched that experienced by both parents. In this study, we found that the exposure of male S. purpuratus to elevated temperatures did not confer any positive effects onto the fertilization process even when fertilization temperatures matched that experienced by the sires. All eggs used in fertilization trials came from dams kept at ambient conditions so we were unable to determine if these fertilization success results changed when both parents experienced a similar stress.
Alternatively, the extent of adaptive paternal effects observed during fertilization may depend on the duration of the adult acclimation to stressful conditions (Stillman 2003; Seebacher et al. 2014). As such, longer (or shorter) exposure of male urchins to the temperatures used in this study could result in additional shifts in the overall fertilization success seen. Guillaume et al. (2016) observed more dramatic decreases in fertilization success when male tubeworms were kept at elevated temperatures for 14 days, half the time we acclimated urchins for in this study. In that same study, larval survival was also measured at 14 days and exhibited a similarly negative response to temperature, but when authors assessed survival again after 28 days, this result had been ameliorated. The research question in this study was fueled by the growing threat of MHWs, which can vary significantly in duration and intensity. As such, these questions would be aided by future experiments directed toward elucidating the extent that paternal (and maternal) effects change in response to temperature over different timescales.
Summary & Implications in a MHW-context
The results of our study suggest MHWs will have a significant impact on marine invertebrate reproduction through alterations to the quality and performance of produced gametes. Other investigators have shown that MHW events can disrupt marine ecosystem structure and function through increased mortality events (Harvell et al. 2019; Seuront et al. 2019), diminished recruitment success (Okamoto et al. 2020; Shanks et al. 2020), and altered ecological interactions (Sanford et al. 2019). In our work, an empirical approach using MHW-like conditions (20 °C) in the lab revealed another potential negative outcome for marine invertebrate populations, altered reproductive output through diminished fertilization success. Paternal acclimation and fertilization treatments in this study were selected based on temperature data collected over the past 6 years at the study sites in which we collect S. purpuratus (Fig. 4). MHW events from 2013 to 2020 were defined using these temperature data and a baseline climatology created from temperatures recorded over the past ~ 17 years (Hobday et al. 2016). During this time, populations of urchins and other marine invertebrates in the Santa Barbara Channel (SBC) have been exposed to multiple MHW events of varying lengths and intensities, with temperatures seen exceeding the 20 °C treatment used here.
Additionally, observations of MHWs in the SBC add more evidence of these events’ seasonality (Sen Gupta et al. 2020). MHWs seen in 2014, 2015, and 2018 all occurred within late summer to Fall, times during which temperatures are at their highest of the year. The timing of these MHWs threatens the overlapping seasonality of S. purpuratus reproduction, where the bulk of gametogenesis occurs from October to January (Strathmann 1987). This phenology places our results in an ecologically relevant context. MHW events can last anywhere from 5 days to multiple months and are only expected to become more frequent and longer in duration over the next decade (Frolicher et al. 2018; Oliver et al. 2018; Smale et al. 2019). Results from this study indicate that current and future MHW events lasting around one month can negatively impact the fertilization success of individual male urchins. While fertilization itself was unaltered by the MHW-like temperatures, pre-spawning exposure of males to elevated temperatures was harmful. If similar effects were seen in nature, the reproductive output of sea urchin populations could be significantly altered by lower numbers of larvae produced or increased selection on particular genotypes from males who are the most competitive under these environments (Campbell et al. 2016). While this study only acknowledges the effect of temperature stress on an early developmental bottleneck, fertilization, negative responses to MHW events within later developmental stages of sea urchins have also been observed (Martino et al. 2021). Additionally, one must also consider the potential of significant carryover effects not explored here, where the effects of this exposure of parents and/or gametes to MHW temperatures could appear much later in development (Byrne and Hernandez 2020). Past MHW events in the SBC already coincided with decreased S. purpuratus biomass and recruitment (Reed et al. 2016; Okamoto et al. 2020). Together these data point toward MHW events as a potentially significant factor in the persistence and structure of sea urchin populations across the CCLME.
Data availability
The datasets generated during and/or analyzed during the current study are available in a Gitbhub repository (https://github.com/tleach32/Spurp-MHW-Fert_2021).
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Acknowledgements
This research was supported by NSF award IOS-1656262 to G.E.H. This work was also supported by resources from Santa Barbara Coastal Long-Term Ecological Research program (NSF award OCE-1831937; Director: Dr. Robert Miller), an award for which GEH is a co-PI. T.S.L. was supported by a Ford Predoctoral Fellowship. The Gorman Scholars Program provided support for B.B. Specimens were collected in the Santa Barbara Channel under a California Scientific Collecting Permit to G.E.H. (SC-1223). Our appreciation goes out to Clint Nelson for collection of animals. We are also extremely grateful for the assistance provided by Logan Kozal, Jannine Chamorro, Xochitl Clare, Samuel Bogan, Amelia Ritger, Adriane McDonald, Cailan Sugano, Avery DeSantis, and Alix Martin during the experiment. We also would like to thank Dr. Kathy Foltz and Dr. Debora Iglesias-Rodriguez for the feedback provided during the writing of this manuscript.
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Conceptualization and design: TSL, GEH; methodology: TSL, BB; data analysis: TSL, BB; writing—original draft preparation: TSL, GEH; writing—review and editing: TSL, BB, GEH. All authors read and approved the final manuscript.
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Leach, T.S., BuyanUrt, B. & Hofmann, G.E. Exploring impacts of marine heatwaves: paternal heat exposure diminishes fertilization success in the purple sea urchin (Strongylocentrotus purpuratus). Mar Biol 168, 103 (2021). https://doi.org/10.1007/s00227-021-03915-x
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DOI: https://doi.org/10.1007/s00227-021-03915-x