Abstract
We explore the phylogeography of the broadcast spawner Marthasterias glacialis along south Europe and Azores. Sequences of the cytochrome c oxidase gene from 225 specimens, belonging to 10 localities, were analysed. We found 73 haplotypes grouped within two lineages (divergence 2.9%). One lineage was Atlanto–Mediterranean, whereas another one was exclusively Mediterranean. Estimation of lineages split goes back to 830,000–580,000 (±120,000) years ago. This suggests that sea-level oscillations during the Pleistocene glaciations promoted gene flow interruption, lineage divergence between basins and cryptic speciation. Secondary contact between populations allowed a recolonization of the Mediterranean by the Atlantic lineage. When animals of the Atlanto–Mediterranean lineage were considered separately, F st index and AMOVA did not show significant differences between populations along either the Iberian Peninsula or basins. Isolation by distance between populations was not detected, and only populations of Plymouth and Azores showed significant differences to all the others. The remoteness of Azores islands might explain the structure of this population. Haphazard arrival of larvae and local extinctions rather than contemporary restricted gene flow might be responsible for the distinctive population structure of Plymouth.
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Acknowledgments
Samples of Marthasterias glacialis from south Portugal were collected during the oceanographic expedition “LUSOEXPEDIÇÃO Olympus 2008” to the “Gorringe Seamounts”. This research was supported by a postdoctoral fellowship from the FCT of Portugal (Ref. SFRH/BPD/41055/2007) funded by the Pluriannual Program (FCT, UI&D 331/94, partially FEDER funded) and the project CMT2007-66635 (MARMOL) from the Spanish Ministry of Science. We strongly thank Xavier Mina for collecting samples from Mutriku, Pablo López for samples from Santander, Gustavo Martins and Joana Micael for samples from Azores, Fernando Roneberg for his help and support during the collection work and Sonia Chenu for her technical assistance in the laboratory.
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Pérez-Portela, R., Villamor, A. & Almada, V. Phylogeography of the sea star Marthasterias glacialis (Asteroidea, Echinodermata): deep genetic divergence between mitochondrial lineages in the north-western mediterranean. Mar Biol 157, 2015–2028 (2010). https://doi.org/10.1007/s00227-010-1470-0
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DOI: https://doi.org/10.1007/s00227-010-1470-0