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Simultaneous determination of chloramphenicol, stilbenes, and resorcylic acid lactones in pork using UPLC–MS/MS with a C18 cartridge and immunoaffinity microextraction in a packed syringe

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Abstract

The aim of this study was to establish an analytical method for the simultaneous detection of chloramphenicol, stilbenes, and resorcylic acid lactones. After enzymatic hydrolysis, pork samples were extracted by ethyl acetate. Two methods were used for purification: a C18 cartridge and immunoaffinity microextraction in a packed syringe. The samples were analyzed by UPLC–MS/MS on an Acquity UPLC® BEH C18 column with gradient elution. The methods were demonstrated to be suitable for the determination of chloramphenicol, stilbenes, and resorcylic acid lactones in pork. The SPE–UPLC–MS/MS had average recoveries of 42.4–97.1%. The intra-day and inter-day RSDs ranged from 6.1 to 16.9% and 3.7 to 18.4%, respectively. The IA–MPES–UPLC–MS/MS had average recoveries of 14.6–33.3%, and the intra-day and the inter-day RSDs ranged from 4.7 to 19.2% and 1.0 to 16.5%, respectively. Overall, the immunoaffinity microextraction in a packed syringe saves the organic reagent when balancing the C18 cartridge; thus, the dose of the organic reagent is less. However, the recovery of extraction by immunoaffinity microextraction in a packed syringe is less ideal.

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Data availability

The authors declare that the data supporting the findings of this study are available within the paper and its supplementary information files.

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Acknowledgements

The authors gratefully thank the National Science Foundation of China (No. 31372482) and Project of Tianjin “131” innovative talent team (20180318) for financial support.

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Authors and Affiliations

  1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, People’s Republic of China

    Shijin Zhao, Leiming Fu, Linyan Yang, Chang Liu, Hongyu Wang, Yan Zhang & Cun Li

  2. Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin, 300381, People’s Republic of China

    Na Li & Yongze Guo

  3. Tianjin Beidagang Wetland Management Center, Tianjin, 300270, People’s Republic of China

    Xinda Zhang

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  1. Shijin Zhao
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Correspondence to Yongze Guo or Cun Li.

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Zhao, S., Fu, L., Yang, L. et al. Simultaneous determination of chloramphenicol, stilbenes, and resorcylic acid lactones in pork using UPLC–MS/MS with a C18 cartridge and immunoaffinity microextraction in a packed syringe. Eur Food Res Technol 250, 1083–1091 (2024). https://doi.org/10.1007/s00217-023-04447-y

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