Skip to main content
Log in

Effect of soy protein isolates inclusion on physicochemical, functional, and nutritional properties of buckwheat gels

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

Soy protein is rich in protein and antioxidant compounds compared to other plant-based foods. Additionally, buckwheat is known as healthy grains which can help strengthen the immune system and inhibit carbohydrate and fat digestion. However, it has a low protein content that can be fortified by soy protein isolate inclusion. The objective of the study was to evaluate the effect of soy protein isolate addition on the physicochemical, functional, and glycaemic properties of the buckwheat gel. In this study, buckwheat gel from buckwheat flour was incorporated with buckwheat bran and soy protein isolate at various levels (5, 10, and 15%) and was analysed for its total starch content, resistant starch, protein content, total phenolic content, antioxidant capacity, pasting properties, and starch digestibility properties. The results show that adding soy protein isolates altered antioxidant and resistant starch content whereas increased protein content and reduced starch digestibility and viscosity of buckwheat gel. Overall, buckwheat flour, buckwheat bran and soy protein isolate blend exhibited buckwheat gel with high nutritive values (rich in protein and antioxidants) and low glycaemic properties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

Availability of data and materials

Data is available upon request.

Code availability

Not applicable.

References

  1. Tien NNT, Trinh LND, Inoue N, Morita N, Hung PV (2018) Nutritional composition, bioactive compounds, and diabetic enzyme inhibition capacity of three varieties of buckwheat in Japan. Cereal Chem 95:615–624

    CAS  Google Scholar 

  2. Gimenez-Bastida JA, Zielinski H (2015) Buckwheat as a functional food and its effects on health. J Agric Food Chem 63:7896–7913

    CAS  PubMed  Google Scholar 

  3. Zhu F (2016) Chemical composition and health effects of Tartary buckwheat. Food Chem 203:231–245

    CAS  PubMed  Google Scholar 

  4. Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 4:118–126

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Georgiev V, Ananga A, Tsolova V (2014) Recent advances and uses of grape flavonoids as nutraceuticals. Nutrients 6:391–415

    PubMed  PubMed Central  Google Scholar 

  6. Jara-Palacios MJ, Hernanz D, Escudero-Gilete ML, Heredia FJ (2016) The use of grape seed byproducts rich in flavonoids to improve the antioxidant potential of red wines. Molecules 21:12

    Google Scholar 

  7. Panche AN, Diwan AD, Chandra SR (2016) Flavonoids: an overview. J Nutr Sci 5:e47

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Cho YJ, Bae IY, Inglett GE, Lee S (2014) Utilization of tartary buckwheat bran as a source of rutin and its effect on the rheological and antioxidant properties of wheat-based products. Ind Crops Prod 61:211–216

    CAS  Google Scholar 

  9. Sindhu R, Khatkar BS (2018) Thermal, structural and textural properties of amaranth and buckwheat starches. J Food Sci Technol 55:5153–5160

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Rachman A, Chen L, Brennan M, Brennan C (2020) Effects of addition of buckwheat bran on physicochemical, pasting properties and starch digestion of buckwheat gels. Eur Food Res Technol 246:2111–2117

    CAS  Google Scholar 

  11. Akinwale TE, Shittu TA, Adebowale A-RA, Adewuyi S, Abass AB (2017) Effect of soy protein isolate on the functional, pasting, and sensory acceptability of cassava starch-based custard. Food Sci Nutr 5:1163–1169

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Zhu Z, Sun C, Wang C, Mei L, He Z, Mustafa S, Du X, Chen X (2024) The anti-digestibility mechanism of soy protein isolate hydrolysate on natural starches with different crystal types. Int J Biol Macromol 255:128213

    CAS  PubMed  Google Scholar 

  13. Waziiroh E, Schoenlechner R, Jaeger H, Brusadelli G, Bender D (2022) Understanding gluten-free bread ingredients during ohmic heating: function, effect and potential application for breadmaking. Eur Food Res Technol 248:1021–1034

    CAS  Google Scholar 

  14. Imran M, Liyan Z (2023) Production of plant-based meat: functionality, limitations and future prospects. Eur Food Res Technol 249:2189–2213

    CAS  Google Scholar 

  15. Cao Z, Liu Y, Zhu H, Li Y, Xiao Q, Yi C (2021) Effect of soy protein isolate on textural properties, cooking properties and flavor of whole-grain flat rice noodles. Foods 10:1085

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Rachman A, Kie S, Brennan MA, Brennan CS (2023) Effect of cassava-banana flour composition and soy protein isolate addition on bread quality and antioxidant properties of gluten-free bread. IOP Conf Ser Earth Environ Sci 1246:012051

    Google Scholar 

  17. AOAC. Official methods of analysis. Method 996.11 Starch (Total) in Cereal Products. AOAC international, Rocville, MD, USA; 1996.

  18. AOAC. Official Methods of Analysis, 17th Ed. Method 2002.02 Resistant Starch Assay Procedure. AOAC International, Rockville, MD, USA; 2002.

  19. Mæhre HK, Dalheim L, Edvinsen GK, Elvevoll EO, Jensen I-J (2018) Protein determination—method matters. Foods 7:5

    PubMed  PubMed Central  Google Scholar 

  20. Yoo KS, Lee EJ, Leskovar D, Patil BS (2012) Development of an automated method for Folin–Ciocalteu total phenolic assay in artichoke extracts. J Food Sci 77:C1279–C1284

    PubMed  Google Scholar 

  21. Lee EJ, Nomura N, Patil BS, Yoo KS (2014) Measurement of total phenolic content in wine using an automatic Folin–Ciocalteu assay method. Int J Food Sci Technol 49:2364–2372

    CAS  Google Scholar 

  22. Siddiqui N, Rauf A, Latif A, Mahmood Z (2017) Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gul-e-Zoofa (Nepeta bracteata Benth). J Taibah Univ Med Sci 12:360–363

    PubMed  PubMed Central  Google Scholar 

  23. Liang W, Wang S, Yao L, Wang J, Gao W (2019) Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric-reducing antioxidant power. J Food Biochem 43:e12901

    PubMed  Google Scholar 

  24. Al-Dabbas MM, Al-Ismail K, Kitahara K, Chishaki N, Hashinaga F, Suganuma T, Tadera K (2007) The effects of different inorganic salts, buffer systems, and desalting of Varthemia crude water extract on DPPH radical scavenging activity. Food Chem 104:734–739

    CAS  Google Scholar 

  25. Hui X, Wu G, Han D, Gong X, Stipkovits L, Wu X, Tang S, Brennan MA, Brennan CS (2021) Bioactive compounds from blueberry and blackcurrant powder alter the physicochemical and hypoglycaemic properties of oat bran paste. LWT 143:111167

    CAS  Google Scholar 

  26. Englyst KN, Hudson GJ, Englyst HN (2006) Starch analysis in food. Encycl Anal Chem Appl Theory Instrum 2006:1

    Google Scholar 

  27. Bindels LB, Munoz RRS, Gomes-Neto JC, Mutemberezi V, Martínez I, Salazar N, Cody EA, Quintero-Villegas MI, Kittana H, de Los Reyes-Gavilán CG (2017) Resistant starch can improve insulin sensitivity independently of the gut microbiota. Microbiome 5:12

    PubMed  PubMed Central  Google Scholar 

  28. Bahmanyar F, Azari M, Hosseini SM, Mirmoghtadaie L, Aliabadi SS (2020) Effects of replacing soy protein and bread crumb with quinoa and buckwheat flour in functional beef burger formulation. Meat Sci 2020:108305

    Google Scholar 

  29. Sharma K, Ko EY, Assefa AD, Ha S, Nile SH, Lee ET, Park SW (2015) Temperature-dependent studies on the total phenolics, flavonoids, antioxidant activities, and sugar content in six onion varieties. J Food Drug Anal 23:243–252

    CAS  PubMed  Google Scholar 

  30. Şensoy Í, Rosen RT, Ho C-T, Karwe MV (2006) Effect of processing on buckwheat phenolics and antioxidant activity. Food Chem 99:388–393

    Google Scholar 

  31. Kang M, Zhai F-H, Li X-X, Cao J-L, Han J-R (2017) Total phenolic contents and antioxidant properties of buckwheat fermented by three strains of Agaricus. J Cereal Sci 73:138–142

    CAS  Google Scholar 

  32. Guo Y, Ma M, Jiang F, Jiang W, Wang H, Du S-K (2020) Protein quality and antioxidant properties of soymilk derived from black soybean after in vitro simulated gastrointestinal digestion. Int J Food Sci Technol 55:720–728

    CAS  Google Scholar 

  33. Wang K, Gao Y, Zhao J, Wu Y, Sun J, Niu G, Zuo F, Zheng X (2022) Effects of in vitro digestion on protein degradation, phenolic compound release, and bioactivity of black bean tempeh. Front Nutr 9:1017765

    PubMed  PubMed Central  Google Scholar 

  34. Wojtunik-Kulesza K, Oniszczuk A, Oniszczuk T, Combrzyński M, Nowakowska D, Matwijczuk A (2020) Influence of in vitro digestion on composition, bioaccessibility and antioxidant activity of food polyphenols—a non-systematic review. Nutrients 12:1401

    CAS  PubMed  PubMed Central  Google Scholar 

  35. Ashaolu TJ (2020) Applications of soy protein hydrolysates in the emerging functional foods: a review. Int J Food Sci Technol 55:421–428

    CAS  Google Scholar 

  36. Bermúdez-Soto M-J, Tomás-Barberán F-A, García-Conesa M-T (2007) Stability of polyphenols in chokeberry (Aronia melanocarpa) subjected to in vitro gastric and pancreatic digestion. Food Chem 102:865–874

    Google Scholar 

  37. Liu D, Guo Y, Wu P, Wang Y, Kwaku Golly M, Ma H (2020) The necessity of walnut proteolysis based on evaluation after in vitro simulated digestion: ACE inhibition and DPPH radical-scavenging activities. Food Chem 311:125960

    CAS  PubMed  Google Scholar 

  38. Cozzolino D (2016) The use of the rapid visco analyser (RVA) in breeding and selection of cereals. J Cereal Sci 70:282–290

    CAS  Google Scholar 

  39. Sindhu R, Khatkar BS (2023) Influence of oxidation, acetylation and hydrothermal treatment on structure and functionality of common buckwheat starch. Int J Biol Macromol 253:127211

    CAS  PubMed  Google Scholar 

  40. Tao J, Wan C, Leng J, Dai S, Wu Y, Lei X, Wang J, Yang Q, Wang P, Gao J (2023) Effects of biochar coupled with chemical and organic fertilizer application on physicochemical properties and in vitro digestibility of common buckwheat (Fagopyrum esculentum Moench) starch. Int J Biol Macromol 246:125591

    CAS  PubMed  Google Scholar 

  41. Ma Q, Zhao Y, Wang H-L, Li J, Yang Q-H, Gao L-C, Murat T, Feng B-L (2020) Comparative study on the effects of buckwheat by roasting: antioxidant properties, nutrients, pasting, and thermal properties. J Cereal Sci 95:103041

    CAS  Google Scholar 

  42. Huang S, Martinez MM, Bohrer BM (2019) The compositional and functional attributes of commercial flours from tropical fruits (breadfruit and banana). Foods 8:586

    CAS  PubMed  PubMed Central  Google Scholar 

  43. Wallace AJ, Monro JA, Hedderley DI, Willis JA, Scott RS (2006) Determining the glycemic glucose equivalent value of foods in humans. Nutr Res 26:47–52

    CAS  Google Scholar 

  44. Zheng M, Xiao Y, Yang S, Liu M, Feng L, Ren Y, Yang X, Lin N, Liu J (2020) Effect of adding zein, soy protein isolate and whey protein isolate on the physicochemical and in vitro digestion of proso millet starch. Int J Food Sci Technol 55:776–784

    CAS  Google Scholar 

Download references

Funding

No funding for the project is declared.

Author information

Authors and Affiliations

Authors

Contributions

AR and YH were responsible for experimental, analysis data production, and writing a draft; AR was responsible for data analysis, supervision, writing of draft; MB was responsible for conceptualization, data analysis, supervision and writing of the draft; CB was responsible for conceptualization, funding, data analysis, supervision and writing of the draft.

Corresponding author

Correspondence to Adetiya Rachman.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Compliance with ethics requirements

The study did not involve human participants or animal objects.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rachman, A., Huang, Y., Brennan, M. et al. Effect of soy protein isolates inclusion on physicochemical, functional, and nutritional properties of buckwheat gels. Eur Food Res Technol 250, 1061–1067 (2024). https://doi.org/10.1007/s00217-023-04445-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-023-04445-0

Keywords

Navigation