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Characterization of ACE inhibitory peptide from Cassia tora L. globulin fraction and its antihypertensive activity in SHR

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Abstract

Cassia tora L. seeds, known as “juemingzi” in China, have been one of the key traditional Chinese medicines for thousands of years. When stir-fried, this herb has an excellent therapeutic effect on hypertension. Research on its active components has mainly focused on flavonoids. Proteins account for approximately 20% of the total seed weight. The effect of protein hydrolysate-derived peptides on blood pressure has not been reported yet. In this study, C. tora peptides from globulin hydrolysate were sequentially separated by ultrafiltration, ion exchange chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC) and identified by the Nano LC-Q-TOF–MS. Then, molecular simulation combining in silico proteolysis was applied to screen the potent sequences. Finally, a novel ACE inhibitory peptide TTPSY was obtained with an IC50 of 5.92 × 10–6 mol/L. In vivo antihypertensive effect of this pentapeptide was evaluated in spontaneously hypertensive rats (SHR). The maximum reduction in blood pressure was 46.00 mmHg at a dose of 10 mg/kg body weight. Echocardiographic assessment revealed the treatment with TTPSY reduced ventricular wall thickness and prevented left ventricle remodeling. Novel peptide TTPSY possessed a satisfactory hypotensive effect and the cardioprotective function, similar to that of captopril, and could be a potential candidate for antihypertensive treatment. Our research provides beneficial data to reveal the pharmacological actions of C. tora.

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Funding

This work was supported by the National Natural Science Foundation of China (NO. 81872972).

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Author notes

  1. Lingzhi Wang and Zujun Chen have contributed equally to this work and share last authorship.

Authors and Affiliations

  1. School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China

    Peiyao Chen, Yueyuan Zhang, Haoyu Shi & Lingzhi Wang

  2. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

    Yue Ren & Yanan Liu

  3. Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, 100037, China

    Zujun Chen

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  1. Peiyao Chen
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Contributions

PC: Investigation, Data curation, Writing-Original draft preparation. YR: Software. YZ: Investigation. YL: Software. HS: Investigation. ZC: Conceptualization, Methodology and Supervision. LW: Conceptualization, Methodology and Supervision. All authors approved it for publication.

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Correspondence to Zujun Chen or Lingzhi Wang.

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Chen, P., Ren, Y., Zhang, Y. et al. Characterization of ACE inhibitory peptide from Cassia tora L. globulin fraction and its antihypertensive activity in SHR. Eur Food Res Technol 248, 1917–1928 (2022). https://doi.org/10.1007/s00217-022-04015-w

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