Abstract
Green-ripe pineapples are shipped overseas by sea freight, while those picked at full maturity need to be transported by airfreight over the same large distance. In this study, fresh-cut pineapple cubes were assessed two, five, and eight days after processing from green-ripe pineapples after mimicked sea freigh (SF) and fully ripe air-freighted (AF) pineapples. The sea-freighted samples displayed elevated titratable acidity (TA), thus resulting in smaller ratios of total soluble solids and TA compared to the AF pineapples. Differences in the carotenoid levels of the two fresh-cut categories were found to be insignificant. By contrast, hierarchical cluster analysis (HCA) and principal component analysis (PCA) calculated on the basis of the volatiles analysed by headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME–GC–MS) permitted to distinguish all six individual sample types and to segregate them into two major clusters (SF and AF). The effect of storage on the volatiles was further evaluated by partial least squares (PLS) regression. Substantial chemical markers to differentiate the individual samples and to describe the effect of storage were deduced from the PCA and PLS regression, respectively. In general, fresh-cut products obtained from fully ripe AF fruit displayed higher concentrations of volatiles, in particular, increased concentrations of diverse methyl esters. With progressing storage duration, the concentrations of ethanol and diverse ethyl esters increased. Moreover, products from AF pineapples displayed lower microbial counts compared to those from SF fruit.
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Introduction
Owing to their exotic flavour, consumers in Europe and North America highly appreciate tropical fruits and products derived thereof. In the pineapple market, fresh fruit and fresh-cut products represent still growing sectors. Two principal logistic chains have been established for supplying the European market with fresh-cut pineapples. The first is based on harvesting fully ripe fruit, which are processed in close proximity to their production sites and then immediately exported by rapid airfreight [1]. For instance, the German and Britain markets are majorly supplied with this premium niche product by two major companies air-freighting fresh-cut fruits from Ghana to Europe [2]. The second chain is based on harvesting early green-ripe pineapples having an extended shelf life under chilled conditions, which permits their transport for multiple weeks by cargo ships. The major proportion of these sea-freighted pineapples is sold on the fresh fruit market. With regard to fresh-cut product manufacture, processing facilities located, e.g., in Europe and North America mostly rely on such sea-freighted fruit.
Pineapple volatiles have been intensely studied during the past decades [3]. In a previous study, fully ripe air-freighted pineapples and three post-harvest stages of green-ripe, sea-freighted fruit were assessed by solid-phase microextraction and gas chromatography–mass spectrometry (HS-SPME–GC–MS), revealing clear-cut differences in their volatiles [4]. In addition, the odour, taste, and overall preference of air-freighted pineapples were significantly better rated by a consumer panel compared to sea-freighted fruit assessed at two post-harvest stages [5]. By analogy to fresh fruit, also quality of fresh-cut products obtained from fully ripe and green-ripe sea-freighted pineapples may differ.
The volatiles of fresh-cut pineapples were investigated by Spanier et al. [6] during a 10-day storage period. Lamikanra [7] reported the involvement of esterases in the development of staleness in sliced pineapples. The effects of ultraviolet (UV) light irradiation [8] and modified atmosphere packaging [9] on fresh-cut pineapple volatiles were assessed. Using an electronic nose, Torri et al. [10] successfully described changes in aroma profiles of cut pineapple flesh stored at different temperatures. However, the influence of harvest maturity and fruit logistics on the composition of volatiles of fresh-cut pineapples has been disregarded in the aforementioned reports.
In the present study, volatiles of fresh-cut pineapple obtained from fully ripe fruit (“air freight”) and pineapples harvested at an earlier green-ripe stage that were stored for 14 days prior to processing (“sea freight”) were monitored by HS-SPME–GC–MS and multivariate statistical data analysis. In addition, drip loss, pH, total soluble solids (TSS), titratable acidity (TA), carotenoid levels, and microbial quality of the two fresh-cut categories were assessed.
Materials and methods
Fresh-cut samples
Green-ripe and fully ripe pineapples (Ananas comosus [L.] Merr. cv. ‘MD2’ (syn. “Extra Sweet”)) were obtained from Jei River Farms (Opeman, Ghana). Green-ripe fruits were harvested ~ 137–145 days after ethylene induced flower induction (synchronisation) at a TSS of ~ 9.5–10 °Brix. According to Ghanaian post-harvest procedures for local market and airfreight export, fruits allowed to ripen on-plant were harvested ~ 147–157 days after synchronisation (~ 11 °Brix), i.e., 10–12 days later than those destined for dispatch by sea freight. For “de-greening”, green-ripe and fully ripe pineapples were treated with approx. 20 and 10 ppm ethylene, respectively, five days prior to harvest. Pineapples of both categories were harvested by hand and brought to the farm (15 km distance from the field) using a pick-up truck. Subsequently, the pineapples were dispatched to the processing facility (ca. 50 km) using a truck. Prior to processing, green-ripe pineapples were stored at 8 °C for 14 days, mimicking their sea freight export from West Africa or Central America to Europe. The fully ripe pineapples were processed within 24 h after harvest.
Flesh cylinders of 83 × 95 mm were obtained with a commercial processing machine. Cores were stamped out using a 26-mm bore hollow knife. The fruit flesh was sliced into rings of 1 cm thickness that were cut into eights. Bits obtained were subsequently dipped into aqueous citric acid solution (20 g/L) and allowed to drip off. Portions of 400 ± 5 g were packed into lockable plastic containers (185 × 145 × 55 mm, Safe-t-fresh, Inline Plastics, Shelton, CT, USA). Following packaging, the fresh-cut samples from green-ripe fruit processed after 14 days of storage at 8 °C and those from fully ripe pineapples were transported to Accra airport and air-freighted to Frankfurt (Germany) lasting approx. 6–7 h. Upon arrival, the samples were dispatched by a forwarding agent to their final destination in Stuttgart. Storage temperature during dispatch and subsequent storage was 8 °C.
Fresh-cut samples were analysed two, five, and eight days after processing (dap). Thus, the relevant shelf life period from the first day of the products´ availability on the European market (2 dap) to the end of the commercial shelf life (8 dap) was considered. Two packages of each fresh-cut category and storage stage were analysed in analytical duplicates (n = 4).
Reagents
Sodium chloride was pre-conditioned at 105 °C for minimum 24 h prior to use. 2-Methyl-1-pentanol, (all-E)-β-carotene, (all-E)-violaxanthin, sodium hydroxide, and potassium hydrogen phthalate were purchased from Sigma Aldrich (Taufkirchen, Germany). Purified water was prepared with an ultrapure water system type arium® 611 UV (Sartorius, Göttingen, Germany) and subsequently boiled for at least 20 min.
Physicochemical characterisation
The drip loss, i.e., the liquid drained from the pineapple chunks was determined gravimetrically. The pH and TSS were assessed with an InoLab 720 pH-meter (WTW, Weilheim, Germany) and a Rx-5000 Abbe refractometer (Atago, Tokyo, Japan), respectively. TA expressed as g citric acid per 100 g of fresh weight (FW) was determined with a Titrino 702 SM (Metrohm, Herisau, Switzerland) titration system [4]. The concentrations of (all-E)-β-carotene and total carotenoids were determined by HPLC–DAD as detailed elsewhere [11]. Retinol activity equivalents (RAEs) were calculated by converting 12 µg dietary (all-E)-β-carotene to 1 µg RAE [12].
Analyses of volatiles
Volatiles were analysed applying an Agilent GC–MS system type 6890 N coupled to an Agilent 5975 Mass Selective Detector (both Agilent Technologies, Santa Clara, CA, USA) as reported previously [4]. Briefly, 110 g pineapple flesh, 10 mL purified water, and 30 g sodium chloride were spiked with the internal standard 2-methyl-1-pentanol and homogenised using a laboratory blender. An aliquot of 8.0 ± 0.1 g of the puree obtained was filled into a 20-mL headspace vial. Volatiles were isolated from the headspace of the sample tempered to 30 °C using a polydimethylsiloxane/divinylbenzene fibre (65 μm PDMS/DVB, StableFlex®, Supelco 57293-U, Sigma Aldrich) during an incubation time of 60 min.
Volatiles were desorbed for 1 min in the splitless mode at a temperature of 250 °C. Carrier gas was helium at a constant flow rate of 1.2 mL/min. GC separation was achieved using a fused silica capillary column coated with a polar polyethylene glycol (PEG) stationary phase (30 m × 0.25 mm, df = 0.25 μm DB-Wax, Agilent J&W Columns, Santa Clara, CA, USA). The temperature program applied was as follows: isothermal hold at 60 °C (1 min), constant raise to 170 °C (3 °C/min) followed by a ramp to 220 °C (10 °C/min) with a final hold time of 10 min. Total run time was 53 min [4]. For validation of peak identity, the system described above was equipped with a fused silica column coated with a slightly polar 5% phenyl-containing polydimethylsiloxane stationary phase (30 m × 0.25 mm, df = 0.25 μm HP-5ms, Agilent J&W Columns). The following temperature program was applied: isothermal hold at 40 °C (1 min), constant raise to 180 °C (5 °C/min) followed by a ramp to 250 °C (10 °C/min) with a final hold time of 5 min. Total run time was 41 min [4].
Individual compounds were identified on the basis of their mass spectra compared to Wiley 6N library (Wiley and Sons, NY, USA) and literature data [13, 14]. Linear retention indices (LRI) according to Van den Dool and Kratz [15] were calculated relative to n-alkanes. Concentrations of the individual volatiles in µg/100 g were expressed as equivalents of the internal standard 2-methyl-1-pentanol.
Microbiological analyses
A representative sample of 25 g of fresh-cut pineapple was taken from a sealed package and blended with 225 mL of sterile physiological NaCl solution in a sterile stomacher bag with lateral filter for 120 s using a Stomacher 400 Circulator (Seward, London, UK). Decimal dilutions of each homogenized sample were prepared in the same diluent and surface spread plated in duplicate on the respective agars. Total aerobic mesophilic plate counts (TPCs) and Pseudomonas spp. viable counts were determined on standard I nutrient (VWR, Darmstadt, Germany) and glutamate starch phenol red (GSP) agar (Merck, Darmstadt, Germany), respectively. Enterobacteriaceae viable counts were assessed using violet red bile dextrose (VRBD) agar (Oxoid, Basingstoke, UK) as previously reported [16]. Yeast and mould plate counts were determined using yeast extract glucose chloramphenicol (YGC) agar (Oxoid). Plates were incubated aerobically at 30 °C for 48 h on standard I and GSP agar. Prior to evaluation, samples surface spread on VRBD and YGS agar were incubated at 37 °C for 24 and 72 h, respectively. Microbial loads were expressed as colony-forming units (CFU) in log scale per gram (log10 CFU/g).
Statistics
Data obtained by HS-SPME–GC–MS were evaluated by PCA, HCA, and PLS regression calculated using Solo software (release 7.3.1, Eigenvector Research, Wenatchee, WA, USA). The PLS regression biplot was drawn with OriginPro 8 (OriginLab, Northampton, MA, USA) as detailed elsewhere [4, 17].
Compounds relevant for class separation in the PCA were calculated by summarising the absolute value of the loadings multiplied with the variance explained by the corresponding principal component [18]. The most discriminative volatiles describing the effect of storage were deduced from the PLS regression using variable identification (VID) coefficients [17, 18].
Significant (p < 0.05) differences of means of the physicochemical traits and the microbial loads were determined by analysis of variance (ANOVA) and Tukey´s test, applying SAS version 9.4 (SAS Institute, Cary, NC, USA).
Results and discussion
Physicochemical characterisation
Physiochemical traits of fresh-cut products from fully ripe air-freighted and green-ripe pineapples after mimicked sea freight are compiled in Table 1. Irrespective of their logistic history, drip loss increased from 5.2–5.4 to 7.2–7.4% with progressing storage duration of all fresh-cut samples. Both fresh-cut categories displayed comparable TSS of 10.50–10.90 and 10.83–11.10 °Brix as determined in the products made from fully and green-ripe pineapples, respectively. By contrast, significantly elevated titratable acids amounting to 0.50–0.53 g/100 g of FW were determined in the samples from green-ripe fruit (fully ripe: 0.36–0.40 g/100 g of FW). Correspondingly, the TSS/TA, approximating the sugar-to-acid ratios of the fully ripe pineapples ranging between 27.5 and 29.9 g/g exceeded those of the sea-freighted samples of merely reaching levels between 21.2 and 22.1 g/g.
The concentrations of (all-E)-β-carotene among all samples amounted to values between 30 and 38 µg/100 g of FW. This provitamin A precursor contributed up to 6–10% of the total carotenoids that ranged between 374 and 518 µg/100 g of FW. The total carotenoids tended to decline at the end of the storage experiment (8 dap), although not reaching statistical significance (p < 0.05). The retinol activity equivalents (RAEs) amounted to 2.5–3.2 µg RAE/100 g of FW, thus being in the range of the 0.3 to 7.3 µg RAE/100 g of FW previously reported in the flesh of ‘Sugar Loaf’, ‘Smooth Cayenne’, ‘MD2’, and ‘Queen Victoria’ pineapples from Ghana [11]. In the present study, the impact of harvest maturity and post-processing storage on the (all-E)-β-carotene and total carotenoid levels of fresh-cut pineapples were found to be insignificant and thus, not further investigated.
HS-SPME–GC–MS profiling analysis
Assignment of fresh-cut pineapple volatiles
Table 2 compiles the HS-SPME–GC–MS data of fresh-cut pineapple volatiles. A total of 131 peaks was detected. Among the tentatively assigned compounds, 91 esters prevailed numerically and quantitatively, comprising 39 and 35 methyl- and ethyl esters, respectively, in addition to 16 acetates as well as 3-methylbutyl hexanoate (64) and ethyl methyl propanedioate (81). Furthermore, 16 terpenes, nine alcohols, six γ-and δ-lactones, five aldehydes, four undecaene hydrocarbons, and two furanones were tentatively identified. This qualitative composition of volatiles resembled that previously reported for fresh ‘MD2’ pineapples [4, 5, 19] and juice obtained thereof [20], except for some juice-specific constituents, such as free fatty acids and diverse norterpenoids, that were not found in the fresh-cut samples assessed herein.
Noteworthy, the concentrations of the volatiles reported in literature as the key odorants of pineapple [21], i.e., ethyl 2-methylpropanoate (no. 8, described to exert a fruity odour), methyl 2-methylbutanoate (11, fruity, apple-like), ethyl 2-methylbutanoate (15, fruity), and 4-hydroxy-2,5-dimethyl-3(2H)-furanone (127, sweet, pineapple-like, caramel-like) were found in elevated concentrations in the samples from fully ripe fruit compared to those obtained from green-ripe pineapples stored for 14 days prior to processing. Applying our analytical method, another key aroma compound of pineapples 1-(3E,5Z)-3,5-undecatriene (52b, fresh, pineapple-like) was not baseline resolved from the abundant ester methyl octanoate and thus, could not be quantitated separately. Moreover, γ- and δ-octalactone (124 and 126, resp.) that have been proposed to exert the distinct coconut-like odour in fully ripe pineapples [22] were found in elevated concentrations in the AF samples compared to those in the SF fresh-cut produce.
Unsupervised classification by PCA and HCA
The effect of harvest maturity and post-harvest procedures on fresh-cut pineapple volatiles was assessed by PCA. The scores and correlation loadings obtained for the first two principal components (PCs) are displayed in Fig. 1a, a′. PC1 and PC2 of the model explained high 80% of variance. PC1 (60%) clearly segregated the fresh-cut samples obtained from green-ripe pineapples after mimicked sea freight (SF) from a second cluster comprising the air-freighted (AF) produce. In addition, the samples were arranged along PC2 (20%) according to the days after processing. This clustering was also observed in the HCA as illustrated by Fig. 1b and the corresponding circles in the PCA scores plot, respectively.
PCA scores (a) and loadings plot (a′) illustrating the effect of harvest maturity and storage duration on fresh-cut pineapple volatiles. The large circles represent the clusters obtained by HCA (b). dap days after processing, AF fresh-cut samples from fruit harvested at a full maturity (“air freight”), SF fresh-cut produce from green-ripe fruit stored for 14 days prior to processing (“sea freight”). Loadings of tentative markers are highlighted by filled circles and labelled with the compound numbers compiled in Table 3
The most discriminative volatiles contributing to the clustering by PCA are compiled in Table 3. Hereby, volatiles with positive loadings on PC1 contribute to the differentiation of the two principal fresh-cut categories, i.e., SF and AF produce. In general, elevated concentrations of volatiles were found in the samples from fully ripe fruit compared to those from their sea-freighted counterparts as can be deduced from the arrangement of the loadings on the right part of the plot. Moreover, two sub-groups of volatiles can be distinguished. Whereas those with positive loadings on PC2, i.e., diverse methyl esters of intermediate chain fatty acids (C6–C10), such as methyl 5-acetoxyhexanoate (119), methyl heptanoate (40), methyl octanoate (52a), methyl (Z)-5-octanoate (59), and methyl decanoate (93), were correlated to the freshly processed samples, volatiles with negative loadings on PC2 contributed to the segregation of the stored produce. In particular, ethanol (6) and diverse ethyl esters of short to intermediate chain fatty acids (C2–C7), such as inter alia ethyl acetate (3), ethyl butanoate (13), ethyl pentanoate (23), ethyl hexanoate (29b), and ethyl (Z)-4-heptenoate (49), were found among the markers contributing to the clustering of the progressed storage stages. Further discriminative volatiles segregating the latter samples were assigned to ethyl esters, i.e., ethyl 3-hydroxybutanoate (76), ethyl methyl propanedioate (82) and the sulphurous volatile ethyl 3-(methylthio)propanoate (86a). The latter was not baseline resolved from the unique pineapple constituent ethyl 3-acetoxy-2-methylbutanoate (86b) [13] on a PEG stationary phase.
In summary, methyl esters prevailed in the fresh samples, whereas diverse ethyl esters were generated with progressing storage duration. This shift from methyl to ethyl esters has been previously observed during post-harvest storage of pineapple fruit and may be triggered by the endogenous and possibly microbial genesis of ethanol [4]. Noteworthy, the differences in the volatiles between fully ripe fruit and those harvested at a green-ripe stage following chilled storage for 14 days were largely retained throughout the entire storage period. To further explore the particular effect of post-processing storage, a PLS regression was calculated as discussed in the following section.
PLS regression
The scores and correlation loadings obtained for the first two latent variables (LVs) of the PLS regression model are illustrated in Fig. 2. As also observed in the PCA, the samples are arranged in two clusters comprising the air-freighted products and those obtained from green-ripe pineapples after mimicked sea freight. In addition, the model clearly fitted an effect of storage as indicated by the high 98% of cumulative variance in the y-variable explained by LV1 (Y = 79%) and LV2 (Y = 19%), as well as by the length of the correlation loadings vector.
PLS regression illustrating the effect of storage on fresh-cut pineapple volatiles. Volatiles with VID ≥|0.80| are displayed as filled circles and labelled with the peak numbers compiled in Table 4
Variable identification (VID) coefficients revealed distinctive volatiles (Table 4). Hereby, positive VIDs indicate the genesis of the respective compound with progressing storage duration. In most instances, such volatiles were alcohols deriving from fermentative metabolic pathways, i.e., ethanol (6), 2-methylpropanol (20), 2-/3-methylbutanol (28ab), and 2,3-butanediol (83). Moreover, the acetates n-propyl acetate (9) and 2-/3-methylbutyl acetate (22ab) in addition to diverse ethyl esters, e.g., ethyl 3-methylbutanoate (16), ethyl 2-hydroxy-3-methylbutanoate (58), and diethyl butanedioate (105) were found as possible markers generated with progressing storage. Vice versa, the concentrations of the volatiles with negative VIDs, here methyl pentanoate (19), methyl (Z)-3-hexenoate (33), and n-hexyl acetate (36) decreased during storage. This may be attributed to transesterification reactions or possibly the enzymatic cleavage of certain esters during storage of fresh-cut pineapples as previously described by Lamikanra [7]. Interestingly, 2,3-butanediol (83) that was found as a putative storage marker of fresh-cut produce represents a precursor of threo- and erythro-2,3-butanediol diacetate that have been reported to arise during post-harvest storage of green-ripe pineapples [4, 13]. Most of the aforementioned volatiles may derive from endogenous metabolic pathways, but also from microbial growth that was assessed in the second part of this study.
Microbiological analysis
To further characterise the influence of post-harvest procedures on the quality of fresh-cut pineapples, total plate counts (TPCs), pseudomonads, Enterobacteriaceae, and the yeast and mould counts were assessed. Enterobacteriaceae were not detected across all samples (not shown in Fig. 3).
Total plate count, TPCs (a), pseudomonads (b), and yeast and mould counts (c) in fresh-cut pineapples displayed as log10 CFU/g. Enterobacteriaceae were not detected among all samples assessed (data not shown). Different letters indicate significant (p < 0.05) differences of means. The dashed line indicates the maximum acceptable plate count for fresh-cut fruit of 6.0 log10 CFU as specified by the IFST [23]
With progressing storage duration, the TPCs increased in both fresh-cut categories (Fig. 3a). However, the TPCs determined in the air-freighted products (AF 3.3–4.4 log10 CFU/g) were significantly smaller than those in their counterparts obtained from pineapples after mimicked sea freight (SF 4.6–6.5 log10 CFU/g). Compared to the TPCs, Pseudomonas spp. viable counts were found at lower levels of 3.2–3.5 log10 CFU/g (AF) and 3.2–4.1 log10 CFU/g (SF) as determined across all air- and sea-freighted samples, respectively. In both sample categories, the pseudomonads only slightly increased during storage (Fig. 3b).
Whereas the initial TPC already differed comparing the AF and SF samples, comparable yeast and mould counts of 3.4 and 3.6 log10 CFU/g, respectively, were found in the two fresh-cut categories assessed 2 dap (Fig. 3c). However, with progressing storage, the yeasts and moulds significantly increased and the plate counts of the sea-freighted samples (SF 5.6 and 6.5 log10 CFU/g as determined 5 and 8 dap, respectively) clearly exceeded those of the air-freighted produce (AF 4.4 and 5.4 log10 CFU/g, resp.).
Yeasts and moulds have been reported to be the most important spoilage microorganisms of fresh-cut fruits [23]. According to the Institute of Food Science and Technology (IFST) [23], the maximum acceptable plate count of 6.0 log10 CFU/g shall not be exceeded during the entire shelf life of sliced fruits stored under chilled conditions. All AF samples assessed displayed microbial loads smaller than 6.0 log10 CFU/g, whereas the TPC as well as the yeast and mould count determined in the SF pineapples exceeded this critical value already five and eight days after processing, respectively (see dashed line in Fig. 3). Noteworthy, the pineapple flesh is not sterile and contains fungi, yeasts, and bacteria [24]. Storage of the green-ripe pineapples prior to processing may result in the elevated TPCs observed in the SF samples, possibly from the growth of this genuine microflora. Concluding, processing freshly harvested pineapples at full maturity resulted in lower total plate as well as yeast and mould counts compared to samples from the green-ripe fruit that were stored for 14 days prior to processing.
Conclusion
With the exception of the drip loss, the titratable acids, and the TSS/TA ratio, post-harvest procedures had only a marginal impact on the physicochemical properties of fresh-cut pineapples. By contrast, processing fully ripe pineapples resulted in lower total plate as well as yeast and mould counts compared to samples from green-ripe fruit stored for 14 days prior to processing. Consequently, processing fully ripe pineapples immediately after harvest may be recommended for fresh-cut processors targeting the premium market rather than using sea-freighted fruit. Moreover, HS-SPME–GC–MS analysis of volatiles allowed the unambiguous distinction between fresh-cut products obtained from fruit harvested at full maturity and green-ripe pineapples stored for 14 days mimicking their sea freight export.
In most instances, elevated concentrations of volatiles were found among the products from fully ripe pineapples. With progressing storage duration, additional volatiles deriving from fermentative metabolic pathways, i.e., diverse alcohols and ethyl esters, were generated.
Concluding, in particular the factors determining the sensory properties, i.e., TSS/TA and the aroma-determining volatiles were the most distinctive traits to discriminate air-freighted fresh-cut pineapples from the produce obtained from sea-freighted fruit. Hence, air-freighted fresh-cut produce may be recommended for consumers that desire to experience the unique aroma quality of fully ripe pineapples. The differing composition of volatiles indicates distinct sensory properties of the two fresh-cut categories assessed that may be subject of ongoing research.
Change history
27 April 2021
A Correction to this paper has been published: https://doi.org/10.1007/s00217-021-03756-4
Abbreviations
- AF:
-
Fully ripe air-freighted pineapple
- dap:
-
Days after processing
- d f :
-
Film thickness
- FW:
-
Fresh weight
- GSP:
-
Glutamate starch phenol red (agar)
- HCA:
-
Hierarchical cluster analysis
- HS-SPME–GC–MS:
-
Headspace solid-phase microextraction–gas chromatography–mass spectrometry
- LRI:
-
Linear retention index
- LV:
-
Latent variable
- PCA:
-
Principal component analysis
- PC:
-
Principal component
- PLS:
-
Partial least squares
- RAE:
-
Retinol activity equivalent
- SF:
-
Green-ripe sea-freighted pineapple
- TA:
-
Titratable acidity
- TPC:
-
Total plate count
- TSS:
-
Total soluble solids
- VID:
-
Variable identification (coefficient)
- VRBD:
-
Violet red bile dextrose (agar)
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Acknowledgements
We gratefully thank Frank Oberschilp (Peelco Ltd., Accra, Ghana) and Fritz Schumacher (Schumacher GmbH, Filderstadt-Bernhausen, Germany) for donating the samples and organisation of the export trial.
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Steingass, C.B., Dickreuter, J., Kuebler, S. et al. Influence of fruit logistics on fresh-cut pineapple (Ananas comosus [L.] Merr.) volatiles assessed by HS-SPME–GC–MS analysis. Eur Food Res Technol 247, 1617–1630 (2021). https://doi.org/10.1007/s00217-021-03718-w
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DOI: https://doi.org/10.1007/s00217-021-03718-w