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Hydrolysis of starches and flours by sorghum malt amylases for dextrins production

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Abstract

Corn and wheat starches as well as wheat and cassava flours were hydrolyzed using sorghum malt at 65 °C for 6 h. During these reactions, dextrose equivalent (DE) values were followed under three concentrations of sorghum malt and calcium chloride. Wheat flour presented the highest DE values and cassava flour had the highest hydrolysis yield. Thus, different dextrins were produced in a pilot plant and were analyzed by HPSEC and HPAEC-PAD for their molecular weight distribution and oligosaccharides composition, respectively. The results indicated that oligosaccharides with broad molecular weight distributions were present in the dextrins produced and that the proportion of maltose was very high.

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References

  1. Vásquez M, Delgado R, Castro AJ (2009) Modeling of the enzymatic hydrolysis of potato (Solanum tuberosum) using response surface methodology. Starch/Stärke 61:601–609

    Article  Google Scholar 

  2. Lin AHM, Chang YH, Chou WB, Lu TJ (2011) Interference prevention in size-exclusion chromatographic analysis of debranched starch glucans by aqueous system. J Agric Food Chem 59:5890–5898

    Article  CAS  Google Scholar 

  3. Sun J, Zhao R, Zeng J, Li G, Li X (2010) Characterization of dextrins with different dextrose equivalents. Molecules 15:5162–5173

    Article  CAS  Google Scholar 

  4. White DR, Hudson P, Adamson JT (2003) Dextrins characterization by high-performance anion-exchange chromatography-pulsed amperometric detection and size-exclusion chromatography-multi-angle light scattering-refractive index detection. J Chromatogr A 997:79–85

    Article  CAS  Google Scholar 

  5. Marchal LM, Beeftink HH, Tramper J (1999) Towards a rational design of commercial maltodextrins. Trends Food Sci Technol 10:345–355

    Article  CAS  Google Scholar 

  6. Castro AM, Castilho LR, Freire DMG (2011) An overview on advances of amylases production and their use in the production of bioethanol by conventional and non conventional processes. Biomass Convers Bioref 1:245–255

    Article  CAS  Google Scholar 

  7. Ayernor GS, Hammond TK, Graffham A (2002) The combination of rice malt and amyloglucosidase for the production of sugar syrup from cassava flour. Afr J Sci Technol 3:11–18

    Google Scholar 

  8. Traoré T, Mouquet C, Icard-Vernière C, Traoré AS, Trêche S (2004) Change in nutrient composition, phytate and cyanide contents and α-amylase activity during cereal malting in small production units in Ouagadougou (Burkina Faso). Food Chem 88:105–114

    Article  Google Scholar 

  9. Dicko MH, Gruppen H, Zouzouho OC, Traoré AS, van Berkel WJH, Voragen AGJ (2006) Effects of germination on the activities of amylases and phenolic enzymes in sorghum varieties grouped according to food end-use properties. J Sci Food Agric 86:953–963

    Article  CAS  Google Scholar 

  10. Ba K, Tine E, Cissé N, Destain J, Thonart P (2010) Étude comparative des composés phénoliques, du pouvoir antioxydant de différentes variétés de sorgho sénégalais et des enzymes amylolytiques de leur malt. Biotechnol Agron Soc Environ 214:131–139

    Google Scholar 

  11. Association of Official Analytical Chemists (AOAC) (1990) Official methods of analysis of the association of official analytical chemists, 15th edn. AOAC, Washington, DC

    Google Scholar 

  12. Ewers E (1965) Determination of starch by extraction and dispersion with hydrochloric acid. International Organization for Standardization, ISO/TC 93/WGL

  13. Miller GL (1959) Use of dinitrosalycilic acid reagent for the determination of reducing sugars. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  14. Chiang SH, Chen CS, Chang CY (2006) Effect of wheat flour protein compositions on the quality of deep-fried gluten balls. Food Chem 97:666–673

    Article  CAS  Google Scholar 

  15. Maache-Rezzoug Z, Zarguili I, Loisel C, Queveau D, Buléon A (2008) Structural modifications and thermal transitions of standard maize starch after DIC hydrothermal treatment. Carbohydr Polym 74:802–812

    Article  CAS  Google Scholar 

  16. Khatoon S, Sreerama YN, Raghavendra D, Bhattacharya S, Bhat KK (2009) Properties of enzymes modified corn, rice and tapioca starches. Food Res Int 42:1426–1433

    Article  CAS  Google Scholar 

  17. Saeleaw M, Schleining G (2010) Effect of blending cassava starch; rice, waxy rice and wheat flour on physic-chemical properties of flour mixtures and mechanical and sound emission properties of cassava crackers. J Food Eng 100:12–24

    Article  CAS  Google Scholar 

  18. Zhou X, Baik BK, Wang R, Lim ST (2010) Retrogradation of waxy and normal corn starch gels by temperature cycling. J Cereal Sci 51:57–65

    Article  CAS  Google Scholar 

  19. Naguleswaran S, Li J, Vasanthan T, Bressler D, Hoover R (2012) Amylolysis of large and small granules of native triticale, wheat and corn starches using a mixture of α-amylase and glucoamylase. Carbohydr Polym 88:864–874

    Article  CAS  Google Scholar 

  20. Charoenkul N, Uttapap D, Pathipanawat W, Takeda Y (2011) Physicochemical characteristics of starches and flours from cassava varieties having different cooked root textures. Food Sci Technol LEB 44:1774–1781

    Article  CAS  Google Scholar 

  21. Charles AL, Huang TC, Lai PY, Chen CC, Lee PP, Chang YH (2007) Study of wheat flour–cassava starch composite mix and the function of cassava mucilage in Chinese noodles. Food Hydrocoll 21:368–378

    Article  CAS  Google Scholar 

  22. Aryee FNA, Oduro I, Ellis WO, Afuakwa JJ (2006) The physicochemical properties of flour samples from the roots of 31 varieties of cassava. Food Control 17:916–922

    Article  CAS  Google Scholar 

  23. Sadeghi A, Shahidi F, Mortazavi SA, Mahalati MN (2008) Evaluation of different parameters effect on maltodextrin production by α-amylase Termamyl 2-x. World Appl Sci J 3:34–39

    Google Scholar 

  24. Chen Y, Huang S, Tang Z, Chen X, Zhang Z (2011) Structural changes of cassava starch granules hydrolyzed by a mixture of α-amylase and glucoamylase. Carbohydr Polym 85:272–275

    Article  CAS  Google Scholar 

  25. Yadav JK (2012) A differential behavior of α-amylase, in terms of catalytic activity and thermal stability, in response to higher concentration CaCl2. Int J Biol Macromol 51:146–152

    Article  CAS  Google Scholar 

  26. Slomiska L, Wisniewska D, Grzeskowiak A (2003) Liquefaction of starch by thermostable α-amylase. ACTA Technol Aliment 2:17–26

    Google Scholar 

  27. Moore GRP, Canto LR, Amante ER (2005) Cassava and corn starch in maltodextrin production. Quim Nova 28:596–600

    Article  CAS  Google Scholar 

  28. Ma Y, Cai C, Wang J, Sun DW (2006) Enzymatic hydrolysis of corn starch producing fat mimetics. J Food Eng 73:297–303

    Article  CAS  Google Scholar 

  29. Sine JP (2010) Applications agroalimentaires et industrielles. In: Enzymologie et applications. Ellipses Edition, Paris, pp 289–295

  30. Wang YJ, Wang L (2000) Structure and properties of commercial maltodextrins from corm, potato and rice starches. Starch/Stärke 52:296–304

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Wallonia-Brussels International. The authors would like to express their thanks to Catherine Chemotti for her technical assistance on HPLC.

Conflict of interest

Khady Ba has received a scholarship from Wallonia Brussels International (WBI). Mario Aguedo, Emmanuel Tine, Michel Paquot, Jacqueline Destain and Philippe Thonart declare no conflict of interest.

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This article does not contain any studies with human or animal subjects.

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Correspondence to Khady Ba.

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Ba, K., Aguedo, M., Tine, E. et al. Hydrolysis of starches and flours by sorghum malt amylases for dextrins production. Eur Food Res Technol 236, 905–918 (2013). https://doi.org/10.1007/s00217-013-1937-6

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  • DOI: https://doi.org/10.1007/s00217-013-1937-6

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