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Relationship between dicotyledone-amaranth, quinoa, fagopyrum, soybean and monocots- sorghum and rice based on protein analyses and their use as substitution of each other

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Abstract

Comparative protein studies of cereals and pseudocereals are important, especially in cases of cereal protein allergy when pseudocereal substitution is unavoidable. Therefore, ten species and cultivars belonging to different Angiosperms families (Oryza sativa normal Poaceae, bran Poaceae and Jasmin Hom Dokmali Poaceae, Sorghum technicum Battand et Traubt Poaceae, Glycine max L. Merr. Fabaceae, Fagopyrum esculentum Mnch. Polygonaceae, Chenopodium quinoa Wild Chenopodiaceae, Amaranthus hybridum v.1004 Amaranthaceae, cruentus v. R104 Amaranthaceae and hypochondriacus v.1023 Amaranthaceae) were examined by sodium dodecyl sulphate (SDS-PAGE) seed protein markers, fluorescence, circular dichroism (CD) spectra and Fourier transform infrared (FT-IR) measurements. A high degree of polymorphism of all species and cultivars was found. Amaranth species have very similar seed protein electrophoretic profiles. According to UPGMA algorithm the examined species and varieties could be clustered into two similarity groups. Soybean, quinoa, buckwheat and Amaranth (as a genus) can be considered as phylogenic distant taxa. The fluorescence properties of amaranth, soybean, quinoa, rice and buckwheat soluble protein fractions were measured by fluorescence of tryptophan at 295 nm, light intensity, peak response and shift in the maximum of emission. Relative structural stabilities of native proteins were estimated by CD and FTIR. Similarities were found between these plants, which could make them a substitution of each other as well as for cereals.

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Acknowledgement

The authors are thankful to Dr. Ivan Goshev, Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria, for his technical assistance in drawing Figure 5

Author information

Authors and Affiliations

  1. Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University-Hadassah Medical School, P. O. B. 12065, Jerusalem, 91120, Israel

    Shela Gorinstein

  2. Plant Breeding and Acclimatization Institute, Radzikow, Poland

    Jerzy Drzewiecki

  3. Department of Chemistry and Biochemistry, Technological Institute of Durango, Durango, Mexico

    Efren Delgado-Licon

  4. Institute of Agricultural Chemistry, Georg-August-University, Goettingen, Germany

    Elke Pawelzik

  5. Departamento de Biotecnologia, Centro de Desarrollo de Productos Bioticos, Instituto Politecnico Nacional, Morelos, Mexico

    Alma Leticia Martinez Ayala

  6. Food Chemistry Department, UPTC, Tunja, Colombia

    Oscar J. Medina

  7. Department of Agricultural Industry, Faculty of Agricultural Technology, King Mondkut Institute of Technology, Ladkrabang, Bangkok, Thailand

    Ratiporn Haruenkit

  8. Kaplan Medical Center, Rehovot, Israel

    Simon Trakhtenberg

Authors
  1. Shela Gorinstein
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  2. Jerzy Drzewiecki
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  3. Efren Delgado-Licon
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  4. Elke Pawelzik
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  5. Alma Leticia Martinez Ayala
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  6. Oscar J. Medina
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  7. Ratiporn Haruenkit
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  8. Simon Trakhtenberg
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Corresponding author

Correspondence to Shela Gorinstein.

Additional information

Author, S. Gorinstein is affiliated with the David R. Bloom Center for Pharmacy.

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Gorinstein, S., Drzewiecki, J., Delgado-Licon, E. et al. Relationship between dicotyledone-amaranth, quinoa, fagopyrum, soybean and monocots- sorghum and rice based on protein analyses and their use as substitution of each other. Eur Food Res Technol 221, 69–77 (2005). https://doi.org/10.1007/s00217-005-1208-2

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  • DOI: https://doi.org/10.1007/s00217-005-1208-2

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