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Microbiological evaluation and molecular characterization of bifidobacteria strains in commercial fermented milks

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Abstract

The aim of this study was to evaluate the presence of Bifidobacterium animalis subsp. lactis in commercial dairy products using different molecular techniques. We analyzed the microbiological composition of 13 commercial fermented milks available in the Spanish market. Thirteen strains of genus Bifidobacterium were isolated from these products and were identified by genus-specific PCR, by fluorescence in situ hybridization (FISH), by multiplex PCR and amplified ribosomal DNA restriction analysis (ARDRA). The same sets of strains were typed by randomly amplified polymorphic DNA (RAPD) analysis and by amplified fragment length polymorphism technique (AFLP). All strains were identified as B. animalis subsp. lactis using ARDRA and multiplex PCR techniques. Similarity between strains was evaluated based on RAPD and AFLP profiles. The isolated strains showed similar profiles by using these techniques, revealing the reduced genetic variability existing among commercial strains, and all these profiles were reproducible in repeated analysis. ARDRA and multiplex PCR are techniques that allow differentiation of the bifidobacteria at genus and species level, but do not indicate if they are different strains, for which reason the RAPD technique is very useful. All bifidobacteria isolated from commercial fermented milks in Spain belong to the same species B. animalis subsp. lactis. Our results demonstrate the necessity to control the presence of bifidobacteria in commercial fermented milks, not only at species level but also at strain level. Multiplex PCR and RAPDs are the most suitable, rapid and precise techniques to identify all bifidobacteria contained in fermented milk products at genus-, species-, and strain levels.

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References

  1. Biavati B, Vescovo M, Torriani S, Bottazzi V (2000) Ann Microbiol 50:117–131

    Google Scholar 

  2. Samona A, Robinson RK (1994) J Soc Dairy Tech 47:58–60

    Article  Google Scholar 

  3. Dave RI, Shah NP (1997) Int Dairy J 7:31–41

    Article  Google Scholar 

  4. Shah NP (2000) J Dairy Sci 83:894–907

    Article  PubMed  CAS  Google Scholar 

  5. Gueimonde M, Delgado S, Mayo B, Ruas-Madiedo P, Margolles A, De los Reyes-Gavilán CG (2004) Food Res Int 37:839–914

    CAS  Google Scholar 

  6. Temmerman R, Pot B, Huys G, Swings J (2003) Int J Food Microbiol 81:1–10

    Article  PubMed  CAS  Google Scholar 

  7. Hamilton-Miller JMT, Shah S, Winkler JT (1999) Public Health Nutr 2:223–229

    Article  PubMed  CAS  Google Scholar 

  8. Roy D, Ward P, Champagne G (1996) Int J Food Microbiol 29:11–29

    Article  PubMed  CAS  Google Scholar 

  9. Chevalier P, Roy D, Ward P (1990) J Appl Bacteriol 68:619–624

    PubMed  CAS  Google Scholar 

  10. Vlkova E, Nevoral J, Jencikova B, Kopecny J, Godefrooij J, Trojanova I, Rada V (2005) J Microbiol Methods 60:365–73

    Article  PubMed  CAS  Google Scholar 

  11. Kokotovic B, On SL (1999) FEMS Microbiol Lett 173:77–84

    Article  PubMed  CAS  Google Scholar 

  12. Charteris WP, Kelly PM, Morelli L, Collins JK (1997) Int J Food Microbiol 35:1–27

    Article  PubMed  CAS  Google Scholar 

  13. Roy D, Ward P, Champagne G (1996) Int J Food Microbiol 29:11–29

    Article  PubMed  CAS  Google Scholar 

  14. Vincent D, Roy D, Mondou F, Dery C (1998) Int J Food Microbiol 43:185–193

    Article  PubMed  CAS  Google Scholar 

  15. Amann RI, Krumholz L, Stahl DA (1990) J Bacteriol 172:762–770

    PubMed  CAS  Google Scholar 

  16. Franks AH, Harmsen HJM, Raangs GC, Jansen GJ, Scut F, Welling GW (1998) Appl Environ Microbiol 64:3336–3345

    CAS  Google Scholar 

  17. Ventura M, Reniero R, Zink R (2001) Appl Environ Microbiol 67:2760–2765

    Article  CAS  Google Scholar 

  18. Ingrassia I, Roques C, Prévots F (2001) Lait 81:263–280

    Article  CAS  Google Scholar 

  19. Woese CR (1987) Microbiol Rev 51:221–271

    PubMed  CAS  Google Scholar 

  20. Zoetendal EG, Akkermans ADL, De Vos WM (1998) Appl Environ Microbiol 64:3854–3859

    CAS  Google Scholar 

  21. Tynkkynen S, Satokari R, Sarela M, Mattila-Sandholm T, Saxelin M (1999) Appl Environ Microbiol 65:3908–3914

    CAS  Google Scholar 

  22. Gibson JR, Slater E, Xerry J, Tompkins DS, Owen RJ (1998) J Clin Microbiol 36:2580–2585

    PubMed  CAS  Google Scholar 

  23. Masco L, Huys G, Gevers D, Verbrugghen L, Swings J (2003) Syst Appl Microbiol 26(4):557–5563

    Article  PubMed  CAS  Google Scholar 

  24. Ventura M, Canchaya C, Meylan V, Klaenhammer TR, Zink R (2003) Appl Environ Microbiol 69(11):6908–6922

    Article  CAS  Google Scholar 

  25. Nebra Y, Blanch AR (1999) Appl Environ Microbiol 65:5173–5176

    CAS  Google Scholar 

  26. Wilson K (1987) In: Ausubel FM, Brent R, Kingston RE, Moore DD, Smith JA, Seidman JG, Struhl K (eds) Current Protocols in Molecular Biology. John Wiley and Sons, New York, USA

    Google Scholar 

  27. Kaufmann P, Pfefferkorn A, Teuber M, Meile L (1997) Appl Environ Microbiol 63:1268–1273

    CAS  Google Scholar 

  28. Langendijk PS, Schut F, Jansen GJ, Raangs GC, Kamphuis GR, Wilkinson MHF, Welling GW (1995) Appl Environ Microbiol 61:3069–3075

    CAS  Google Scholar 

  29. Lynch PA, Gilpin BJ, Sinton LW, Savill MG (2002) J Appl Microbiol 92:526–533

    Article  PubMed  CAS  Google Scholar 

  30. Bouhnik Y (1993) Lait 73:241–247

    Article  Google Scholar 

  31. FAO/WHO (2002) Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria. Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria

  32. Grand M, Küffer M, Baumgartner A (2003) Eur Food Res Technol 217:90–92

    Article  CAS  Google Scholar 

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Acknowledgements

An FPU/MEC (Spain) scholarship to M.C. Collado is fully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Polytechnic University of Valencia, Camino de Vera 14, 46022, Valencia, Spain

    M. Carmen Collado, Yolanda Moreno & Manuel Hernández

  2. Red INDE, Investigación Nutricional Danone España, Barcelona, Spain

    José M. Cobo

Authors
  1. M. Carmen Collado
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  2. Yolanda Moreno
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  3. José M. Cobo
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  4. Manuel Hernández
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Correspondence to Manuel Hernández.

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Collado, M.C., Moreno, Y., Cobo, J.M. et al. Microbiological evaluation and molecular characterization of bifidobacteria strains in commercial fermented milks. Eur Food Res Technol 222, 112–117 (2006). https://doi.org/10.1007/s00217-005-0093-z

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  • DOI: https://doi.org/10.1007/s00217-005-0093-z

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