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Fluorescent aptasensor mediated with multiple ssDNA for sensitive detection of acetamiprid in vegetables based on magnetic Fe3O4/C-assisted separation

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Abstract

Acetamiprid (ACE) is a highly effective broad-spectrum insecticide, and its widespread use is potentially harmful to human health and environmental safety. In this study, magnetic Fe3O4/carbon (Fe3O4/C), a derivative of metal–organic framework MIL-101 (Fe), was synthesized by a two-step calcination method. And a fluorescent sensing strategy was developed for the efficient and sensitive detection of ACE using Fe3O4/C and multiple complementary single-stranded DNA (ssDNA). By using aptamer with multiple complementary ssDNA, the immunity of interference of the aptasensor was improved, and the aptasensor showed high selectivity and sensitivity. When ACE was present, the aptamer (Apt) combined with ACE. The complementary strand of Apt (Cs1) combined with two short complementary strands of Cs1, fluorophore 6-carboxyfluorescein-labeled complementary strand (Cs2-FAM) and the other strand Cs3. The three strands formed a double-stranded structure, and fluorescence would not be quenched by Fe3O4/C. In the absence of ACE, Cs2-FAM would be in a single-chain state and would be adsorbed by Fe3O4/C, and the fluorescence of FAM would be quenched by Fe3O4/C via photoelectron transfer. This aptasensor sensitively detected ACE over a linear concentration range of 10–1000 nM with a limit of detection of 3.41 nM. The recoveries of ACE spiked in cabbage and celery samples ranged from 89.49% to 110.76% with high accuracy.

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Funding

This work was supported by the National Natural Science Foundation of China (No.32372438, 31772068), the Central Finance Guidance Local Science and Technology Development Fund Project (YDZX2022163), the Natural Science Foundation of Shandong Province (ZR2023MC088), the Shandong Province Major Applied Technology Innovation Project (SD2019NJ007), the Technological Innovation Guidance Project of the Department of Science &Technology of Gansu Province (22CX8NA023), and the Weifang Science and Technology Development Project (2021ZJ1103).

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Authors and Affiliations

  1. School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, 255049, Shandong, China

    Baoxin Li, Mengyue Liu, Lingjun Geng, Shouyi Dou, Shengxi Zhai, Jingjing Liu, Jiashuai Sun, Wenping Zhao, Yemin Guo & Xia Sun

  2. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, 255049, Shandong, China

    Baoxin Li, Mengyue Liu, Lingjun Geng, Shouyi Dou, Shengxi Zhai, Jingjing Liu, Jiashuai Sun, Wenping Zhao, Yemin Guo & Xia Sun

  3. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, 255049, Shandong, China

    Baoxin Li, Mengyue Liu, Lingjun Geng, Shouyi Dou, Shengxi Zhai, Jingjing Liu, Jiashuai Sun, Wenping Zhao, Yemin Guo & Xia Sun

  4. Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, China

    Haifang Wang

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  1. Baoxin Li
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Contributions

B.L.: conceptualization, methodology, formal analysis, and writing, original draft. H.W.: investigation, formal analysis, and validation. M.L.: writing, review and editing, and investigation. L.G.: investigation, formal analysis, and validation. S.D.: investigation and resources. S.Z.: investigation and resources. J.L.: investigation and resources. J.S.: investigation and resources. W.Z.: writing, review and editing. Y.G.: writing, review and editing, supervision, project administration, and funding acquisition. X.S.: writing, review and editing.

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Correspondence to Yemin Guo.

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Li, B., Wang, H., Liu, M. et al. Fluorescent aptasensor mediated with multiple ssDNA for sensitive detection of acetamiprid in vegetables based on magnetic Fe3O4/C-assisted separation. Anal Bioanal Chem 416, 1105–1115 (2024). https://doi.org/10.1007/s00216-023-05104-9

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