Abstract
Zearalenone (ZEN), a resorcinolactone toxin, which has been a potential threat to agricultural production and human health. In this study, a sample and rapid fluorescence sensor was established for the detection of ZEN, which is based on the fluorescence properties of N-doped carbon dots-aptamer (NCDs-apt) and the quenching ability of oxidized single-walled carbon nanohorns (oxSWCNHs). NCDs synthesized by one-step hydrothermal method were connected with ZEN-aptamer (ZEN-apt), and oxSWCNHs were added to quench the fluorescence of NCDs-apt. Therefore, an oxSWCNHs/NCDs-apt aptasensor based on fluorescence “on-off” for the determination of ZEN in food was formed. Under optimum conditions, the limit of detection (LOD) of this method was 18 ng/mL and the linear range was 20 ~ 100 ng/mL. The possible interfering substances were investigated, and the results showed excellent selectivity. The recoveries were in the range of 99.5%~114.3%, and the relative standard deviations (RSDs) were not more than 6.5%, which demonstrated that this aptasensor was successfully applied for the detection of ZEN in food samples with satisfactory result.
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Funding
This work was supported by the Youqing lift Program of Shenyang Pharmaceutical University (YQ202206) and the Middle-aged Backbone Personnel Training Program of Shenyang Pharmaceutical University (ZQN2016011).
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Yue Na: Methodology, Validation, Investigation, Formal analysis, Writing-original draft, Preparing revision. Jiaxin Zhang: Methodology, Validation, Investigation, Formal analysis, Writing-original draft, Preparing revision. Shunhua Zhang: Data curation, Software. Ning Liang: Methodology, Supervision, Resources, Writing-review & editing. Longshan Zhao: Methodology, Supervision, Resources, Writing-review & editing.
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Na, Y., Zhang, J., Zhang, S. et al. Fluorescence Sensor for Zearalenone Detection Based on Oxidized Single-walled Carbon Nanohorns/N-doped Carbon Quantum Dots-aptamer. J Fluoresc (2023). https://doi.org/10.1007/s10895-023-03466-y
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DOI: https://doi.org/10.1007/s10895-023-03466-y