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Determination of proflavine in rat whole blood without sample pretreatment by laser desorption postionization mass spectrometry

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Abstract

A novel pretreatment-free method involving laser desorption postionization (LDPI) coupled with time-of-flight mass spectrometry (MS) was developed for the monitoring of proflavine level in rat whole blood. It comprises a protocol for dosing via intravenous administration and collection of whole blood, followed by direct LDPI-MS analysis without any sample pretreatment. An intense ion signal at m/z 209 was observed from whole blood without any interference signals, except some background signals below m/z 100. The calibration curve was established with use of 9-phenylacridine as the internal standard for proflavine determination from the plotting of the peak ratios of proflavine to the internal standard, with a correlation coefficient (R 2) greater than 0.99. The limit of detection was estimated to be 0.48 pmol/mm2 and the quantification range was 0.5-16.5 μg/mL for proflavine. In addition, only a minimal matrix effect was observed, as expected from considerations of the desorption and ionization mechanism. Interday and intraday accuracy and precision were calculated to be within 13% and 82–114%, respectively. Estimated concentrations of proflavine residue in whole blood were also successfully obtained at selected time points after dosing. The proposed method is simple, low cost, and sensitive, and should be seen as a complementary method for monitoring drug levels in blood.

Monitoring proflavine levels in rat whole blood at different time points using laser desorption postionization mass spectrometry (LDPI-MS)

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Acknowledgements

This research was supported by National Natural Science Foundation of China grants (nos. 21273083 and U1332132), and the Scientific and Technological Planning Project of Guangzhou City.

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Authors and Affiliations

  1. MOE Key laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, Guangdong, 510631, China

    Jiaxin Chen, Yongjun Hu, Qiao Lu, Pengchao Wang & Huaqi Zhan

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  1. Jiaxin Chen
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  2. Yongjun Hu
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  3. Qiao Lu
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  4. Pengchao Wang
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  5. Huaqi Zhan
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Correspondence to Yongjun Hu.

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Chen, J., Hu, Y., Lu, Q. et al. Determination of proflavine in rat whole blood without sample pretreatment by laser desorption postionization mass spectrometry. Anal Bioanal Chem 409, 2813–2819 (2017). https://doi.org/10.1007/s00216-017-0225-x

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  • DOI: https://doi.org/10.1007/s00216-017-0225-x

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