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Quantitative bioimaging of trace elements in the human lens by LA-ICP-MS

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Abstract

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used for the quantitative imaging of Fe, Cu and Zn in cryostat sections of human eye lenses and for depth profiling analysis in bovine lenses. To ensure a tight temperature control throughout the experiments, a new Peltier-cooled laser ablation cell was employed. For quantification purposes, matrix-matched laboratory standards were prepared from a pool of human lenses from eye donors and spiked with standard solutions containing different concentrations of natural abundance Fe, Cu and Zn. A normalisation strategy was also carried out to correct matrix effects, lack of tissue homogeneity and/or instrumental drifts using a thin gold film deposited on the sample surface. Quantitative images of cryo-sections of human eye lenses analysed by LA-ICP-MS revealed a homogeneous distribution of Fe, Cu and Zn in the nuclear region and a slight increase in Fe concentration in the outer cell layer (i.e. lens epithelium) at the anterior pole. These results were assessed also by isotope dilution mass spectrometry, and Fe, Cu and Zn concentrations determined by ID-ICP-MS in digested samples of lenses and lens capsules.

Depth profiling analysis and quantitative imaging analyses of Fe, Cu and Zn in eye lens sections by LA-ICP-MS using matrix-matched laboratory standards for external calibration and 197Au+ as internal standard

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Acknowledgments

Financial support from “Plan Nacional de I+D+I” (Spanish Ministry of Science and Innovation or MICINN, and FEDER Program) through MAT2010-20921-C02-01 and PCTI Asturias through the project FC-09-EQUIP09-29 is acknowledged. I. Konz and B. Fernández thank the FPU program (MICINN) and FC-11-COF11-21 project (PCTI Asturias), respectively, for financial support. M. Coca-Prados and H. González-Iglesias acknowledge financial support from “Fundación de Investigación Oftalmológica”, “Fundación Rafael del Pino” and “Fundación Ma. Cristina Masaveu Paterson”. Finally, the authors gratefully acknowledge Lydia Alvarez from Fundación de Investigación Oftalmológica, Instituto Oftalmológico Fernández-Vega.

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Authors and Affiliations

  1. Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julian Clavería, 8, 33006, Oviedo, Spain

    Ioana Konz, Beatriz Fernández, M. Luisa Fernández, Rosario Pereiro & Alfredo Sanz-Medel

  2. Fundación de Investigación Oftalmológica, Instituto Oftalmológico Fernández-Vega, Avenida Doctores Fernández-Vega, 34, 33012, Oviedo, Spain

    Héctor González-Iglesias & Miguel Coca-Prados

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  1. Ioana Konz
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  2. Beatriz Fernández
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  3. M. Luisa Fernández
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  4. Rosario Pereiro
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  5. Héctor González-Iglesias
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  6. Miguel Coca-Prados
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  7. Alfredo Sanz-Medel
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Corresponding authors

Correspondence to Beatriz Fernández or Alfredo Sanz-Medel.

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Konz, I., Fernández, B., Fernández, M.L. et al. Quantitative bioimaging of trace elements in the human lens by LA-ICP-MS. Anal Bioanal Chem 406, 2343–2348 (2014). https://doi.org/10.1007/s00216-014-7617-y

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  • DOI: https://doi.org/10.1007/s00216-014-7617-y

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