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A resonance light scattering sensor based on methylene blue–sodium dodecyl benzene sulfonate for ultrasensitive detection of guanine base associated mutations

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Abstract

A resonance light scattering (RLS) sensor for guanine base associated mutations has been developed on the basis of the high selectivity of methylene blue (MB) for guanine bases in the presence of sodium dodecyl benzene sulfonate (SDBS). MB, when bound to SDBS, underwent a dramatic enhancement of its RLS intensity. However, the addition of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) caused the strong RLS intensity of MB–SDBS to decrease, and the RLS intensity of MB–SDBS–ssDNA was much lower than that of MB–SDBS–dsDNA. Consequently, it can be concluded that the binding abilities of MB–SDBS with ssDNA and dsDNA were different. Besides, the experimental results showed that MB–SDBS could bind specifically to oligonucleotides rich in guanine bases. Short DNA targets with sequences related to β-thalassaemia, thrombophilia and psoriasis, all of which are guanine base relevant mutations, were synthesized. It was found that MB–SDBS could recognize the single-base mismatches in the mutational DNA, followed by different RLS signal changes between MB–SDBS–normal DNA systems and MB–SDBS–mutational DNA systems. The ultrasensitive sensor allows simple, rapid, sensitive and selective detection of guanine base associated mutations, indicating its potential application in the medical field.

An RLS sensor for the detection of guanine base associated mutations

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Acknowledgments

The authors express their sincere thanks for the financial support from the Guangdong Science and Technology Department (no. 2006B35630009) and the Science Foundation of Shantou University (no. YR09009).

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Authors and Affiliations

  1. Department of Chemistry, Shantou University, Shantou, 515063, China

    Zhanguang Chen, Sihua Qian & Liwen Zheng

  2. Interventional Oncology & Minimally Invasive Therapies Department, Peking University Shenzhen Hospital, Shenzhen, 518036, China

    Junhui Chen

  3. Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Xi Chen

  4. Department of Chemistry, The University of Texas at Dallas, Richardson, TX, 75080, USA

    Jinbin Liu

Authors
  1. Zhanguang Chen
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  2. Sihua Qian
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  3. Junhui Chen
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  4. Xi Chen
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  5. Liwen Zheng
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  6. Jinbin Liu
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Correspondence to Zhanguang Chen.

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Chen, Z., Qian, S., Chen, J. et al. A resonance light scattering sensor based on methylene blue–sodium dodecyl benzene sulfonate for ultrasensitive detection of guanine base associated mutations. Anal Bioanal Chem 404, 1673–1679 (2012). https://doi.org/10.1007/s00216-012-6289-8

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  • DOI: https://doi.org/10.1007/s00216-012-6289-8

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