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A resonance light-scattering off–on system for studies of the selective interaction between adriamycin and DNA

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Abstract

On the basis of the resonance light scattering (RLS) of Ag nanoparticles (AgNPs), an RLS off–on system was developed for studies of the selective interaction between adriamycin (ADM) and DNA. In this strategy, addition of ADM could induce a proportional decrease in the RLS intensity of AgNPs; this could be used to detect trace amounts of ADM with a detection limit of 12.75 ng mL−1 in the range 0.021–10.0 μg mL−1. Subsequently, by investigating the ability of different DNA sequences to restore the RLS intensity of the analytical systems, we found that ADM was selective to dsDNA and had an obvious preference for sequences that were rich in guanine and cytosine bases. In order to validate the results of the RLS assay, fluorescence quenching was used, and binding constants and binding numbers of each system were calculated. Compared with other methods, this RLS off–on strategy was more sensitive, fast, and reliable. It has also supplied a novel method for studying the sequence selectivity of DNA-targeted anticancer drugs and is a novel application of the RLS technique in analytical chemistry.

A resonance light scattering off-on system for the studies of selective interaction between adriamycin and DNA

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Acknowledgments

All the authors are grateful for financial support from the Guangdong Science and Technology Department (no. 2006B35630009) and the Science Foundation of Shantou University (no. YR09009).

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Authors and Affiliations

  1. Department of Chemistry, Shantou University, Shantou, 515063, China

    Zhanguang Chen & Guoming Zhang

  2. Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Xi Chen

  3. Analysis and Testing Center, Shantou University, Shantou, 515063, China

    Wenhua Gao

Authors
  1. Zhanguang Chen
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  2. Guoming Zhang
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  3. Xi Chen
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  4. Wenhua Gao
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Correspondence to Zhanguang Chen.

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Chen, Z., Zhang, G., Chen, X. et al. A resonance light-scattering off–on system for studies of the selective interaction between adriamycin and DNA. Anal Bioanal Chem 402, 2163–2171 (2012). https://doi.org/10.1007/s00216-011-5672-1

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  • DOI: https://doi.org/10.1007/s00216-011-5672-1

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