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Use of synchrotron-radiation-based FTIR imaging for characterizing changes in cell contents

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Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

FTIR imaging of individual cells is still limited by the low signal-to-noise ratio obtained from analysis of such weakly absorbing organic matter when using a Globar IR source. In this study, we used FTIR imaging with a synchrotron radiation source and a focal plane array detector to determine changes in the cellular contents of cryofixed cells after culture for 48 h on Si3N4 substrate. Several spectral differences were observed for cells deprived of glucose compared with control cells: a lower amide I-to-amide II ratio (P < 0.01); a different secondary structure profile of proteins (obtained from amide I spectral region curve fitting), with a significant increase in non-ordered structure components (P < 0.01); and a higher ν(C = C–H)/ν as(CH3) absorption ratio (P < 0.01), suggesting increased unsaturation of fatty acyl chains. Therefore, our study has shown that FTIR imaging with a synchrotron radiation source enables determination of several spectral changes of individual cells between two experimental conditions, which thus opens the way to cell biology studies with this vibrational spectroscopy technique.

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Abbreviations

FPA:

Focal plane array

FTIR:

Fourier-transform infrared

SNR:

Signal-to-noise ratio

SR:

Synchrotron radiation

References

  1. Castano S, Delord B, Fevrier A, Lehn JM, Lehn P, Desbat B (2009) Biochimie 91:765–773

    Article  CAS  Google Scholar 

  2. Castano S, Desbat B (2005) Biochim Biophys Acta 1715:81–95

    Article  CAS  Google Scholar 

  3. Petibois C, Déléris G (2006) Trends Biotechnol 24:455–462

    Article  CAS  Google Scholar 

  4. Petibois C, Desbat B (2010) Trends Biotechnol 28:495–500

    Article  CAS  Google Scholar 

  5. Petibois C, Cazorla G, Cassaigne A, Deleris G (2001) Clin Chem 47:730–738

    CAS  Google Scholar 

  6. Jamin N, Dumas P, Moncuit J, Fridman WH, Teillaud JL, Carr GL, Williams GP (1998) Proc Natl Acad Sci U S A 95:4837–4840

    Article  CAS  Google Scholar 

  7. Fernandez DC, Bhargava R, Hewitt SM, Levin IW (2005) Nat Biotechnol 23:469–474

    Article  CAS  Google Scholar 

  8. Daudon M, Marfisi C, Lacour B, Bader C (1991) Clin Chem 37:83–87

    CAS  Google Scholar 

  9. Naumann D, Helm D, Labischinski H (1991) Nature 351:81–82

    Article  CAS  Google Scholar 

  10. Cestelli Guidi M, Yao S, Sali D, Castano S, Marcelli A, Petibois C (2012) Biotechnol Adv doi:10.1016/j.biotechadv.2011.11.009

  11. Petibois C, Deleris G, Piccinini M, Cestelli Guidi M, Marcelli A (2009) Nat Photonics 3:179

    Article  CAS  Google Scholar 

  12. Petibois C (2010) Anal Bioanal Chem 397:2031–2032

    Article  CAS  Google Scholar 

  13. Petibois C, Piccinini M, Cestelli-Guidi M, Marcelli A (2010) J Synchrotron Rad 17:1–11

    Article  CAS  Google Scholar 

  14. Petibois C, Cestelli Guidi M, Piccinini M, Moenner M, Marcelli A (2010) Anal Bioanal Chem 397:2123–2129

    Article  CAS  Google Scholar 

  15. Weksler BB, Subileau EA, Perriere N, Charneau P, Holloway K, Leveque M, Tricoire-Leignel H, Nicotra A, Bourdoulous S, Turowski P, Male DK, Roux F, Greenwood J, Romero IA, Couraud PO (2005) FASEB J 19:1872–1874

    CAS  Google Scholar 

  16. Barnes PR, Taylor DJ, Kemp GJ, Radda GK (1993) J Neurol Neurosurg Psychiatry 56:679–683

    Article  CAS  Google Scholar 

  17. Nelander B, Sablinskas V (1995) J Mol Struct 348:167–170

    Article  CAS  Google Scholar 

  18. Belbachir K, Noreen R, Gouspillou G, Petibois C (2009) Anal Bioanal Chem 385:829–837

    Article  Google Scholar 

  19. Petibois C, Déléris G (2004) Analyst 129:912–916

    Article  CAS  Google Scholar 

  20. Petibois C, Cassaigne A, Gin H, Deleris G (2004) J Clin Endocrinol Metab 89:3377–3384

    Article  CAS  Google Scholar 

  21. Petibois C, Déléris G (2005) Cell Biol Int 29:709–716

    Article  CAS  Google Scholar 

  22. Noreen R, Moenner M, Hwu Y, Petibois C (2012) Biotechnol Adv doi:10.1016/j.biotechadv.2012.03.009

  23. Wehbe K, Pinneau R, Moenner M, Deleris G, Petibois C (2008) Anal Bioanal Chem 392:129–135

    Article  CAS  Google Scholar 

  24. Petibois C, Gouspillou G, Wehbe K, Delage JP, Deleris G (2006) Anal Bioanal Chem 386:1961–1966

    Article  CAS  Google Scholar 

  25. Goormaghtigh E, Raussens V, Ruysschaert JM (1999) Biochim Biophys Acta 1422:105–185

    Article  CAS  Google Scholar 

  26. Wood BR, Quinn MA, Tait B, Ashdown M, Hislop T, Romeo M, McNaughton D (1998) Biospectroscopy 4:75–91

    Article  CAS  Google Scholar 

  27. Petibois C, Déléris G (2005) Arch Med Res 36:524–531

    Article  CAS  Google Scholar 

  28. Severcan F, Gorgulu G, Gorgulu ST, Guray T (2005) Anal Biochem 339:36–40

    Article  CAS  Google Scholar 

  29. Surewicz WK, Mantsch HH, Chapman D (1993) Biochemistry 32:389–394

    Article  CAS  Google Scholar 

  30. Troullier A, Reinstadler D, Dupont Y, Naumann D, Forge V (2000) Nat Struct Biol 7:78–86

    Article  CAS  Google Scholar 

  31. Drogat B, Bouchecareilh M, Petibois C, Déléris G, Chevet E, Bikfalvi A, Moenner M (2007) J Cell Physiol 212:463–472

    Article  CAS  Google Scholar 

  32. Petibois C, Drogat B, Bikfalvi A, Deleris G, Moenner M (2007) FEBS Let 581:5469–5474

    Article  CAS  Google Scholar 

  33. Brauns EB, Dyer RB (2005) Biophys J 89:3523–3530

    Article  CAS  Google Scholar 

  34. Fabian H, Naumann D (2004) Methods 34:28–40

    Article  CAS  Google Scholar 

  35. Schultz CP, Barzu O, Mantsch HH (2000) Appl Spectrosc 54:931–938

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are indebted to the “Ligue Nationale contre le cancer” and the “Agence Nationale de la Recherche” (ANR contract no. bl-inter09_464249 – MIAG-X) for financial support. This research was also supported within the EU 7th Framework Programme (FP7/2007-2013) under the grant agreement no. 226716.

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Authors and Affiliations

  1. Université de Bordeaux, CNRS UMR 5248, Allée de St Hillaire, 33600, Pessac-Cedex, France

    Seydou Yao & Cyril Petibois

  2. Université de Bordeaux, CNRS UMR 5095, 1, rue Camille Saint Saëns, 33077, Bordeaux-Cedex, France

    Michel Moenner

  3. MAX-lab - Lund University, P.O. Box 118, 22100, Lund, Sweden

    Anders Engdahl

Authors
  1. Seydou Yao
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  2. Michel Moenner
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  3. Anders Engdahl
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  4. Cyril Petibois
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Corresponding author

Correspondence to Cyril Petibois.

Additional information

Published in the special paper collection Imaging Techniques with Synchrotron Radiation with guest editor Cyril Petibois.

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Yao, S., Moenner, M., Engdahl, A. et al. Use of synchrotron-radiation-based FTIR imaging for characterizing changes in cell contents. Anal Bioanal Chem 404, 1311–1316 (2012). https://doi.org/10.1007/s00216-012-6223-0

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  • DOI: https://doi.org/10.1007/s00216-012-6223-0

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