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Use of enriched 74Se and 77Se in combination with isotope pattern deconvolution to differentiate and determine endogenous and supplemented selenium in lactating rats

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Abstract

A quantitative methodology has been developed to differentiate between endogenous and supplemented selenium in lactating rats using two enriched selenium isotopes. Lactating rats were fed for 2 weeks with formula milk containing one enriched Se isotope, 77Se, as the metabolic tracer. The isotopic composition of selenium in serum and urine samples was then measured by collision cell ICP-MS after the addition of a solution containing another enriched isotope, 74Se, as quantitation tracer, before analysis. Isotope pattern deconvolution allowed the transformation of measured Se isotopic abundances into concentrations of natural abundance (endogenous) selenium and enriched 77Se (supplemented) present in the samples. The proposed methodology was validated using serum and urine reference materials spiked with both 77Se and 74Se. The obtained results are discussed in terms of selenium exchange and half-life in lactating rats (11–12 days) and selenium levels in serum in comparison with non-supplemented rats and control rats after maternal feeding.

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Acknowledgements

The authors are grateful to Project PC-REC01-13 of the ‘Fundación para la Investigación Científica Aplicada y la Tecnología, Principado de Asturias (FICYT)’ and to LABORATORIOS ORDESA (Barcelona, Spain) and Fundación Grupo Castrillo (Spain) for financial support.

Additional financial support from Ministerio de Ciencia y Tecnología (Projects, 06-BQU-0468 and CTQ2006-05722) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain

    H. González Iglesias, M. L. Fernández Sánchez, J. I. García Alonso & A. Sanz-Medel

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  1. H. González Iglesias
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  2. M. L. Fernández Sánchez
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  3. J. I. García Alonso
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  4. A. Sanz-Medel
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Correspondence to A. Sanz-Medel.

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González Iglesias, H., Fernández Sánchez, M.L., García Alonso, J.I. et al. Use of enriched 74Se and 77Se in combination with isotope pattern deconvolution to differentiate and determine endogenous and supplemented selenium in lactating rats. Anal Bioanal Chem 389, 707–713 (2007). https://doi.org/10.1007/s00216-007-1499-1

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  • DOI: https://doi.org/10.1007/s00216-007-1499-1

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