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Three commercial antibodies against α1-adrenergic receptor subtypes lack specificity in paraffin-embedded sections of murine tissues

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Abstract

We tested the specificity of three commercially available antibodies (AB) against individual α1-adrenergic receptor subtypes (α1-ARST). We used these ABs to localize the α1-ARST proteins by immunohistochemistry in paraffin-embedded murine tissues. The specificity of the ABs was tested by comparing staining patterns in tissues from wild-type mice with those in corresponding tissues from mice with gene-targeted disruption of the respective α1-ARST, one of the most rigorous negative controls. None of the tested ABs proved to be specific for the indicated target antigen. We conclude that the tested ABs are unsuitable for immunohistochemistry in paraffin-embedded murine tissues.

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Authors and Affiliations

  1. Department of Ophthalmology, University Medical Center, Johannes Gutenberg University Mainz, Langenbeckstr. 1, 55131, Mainz, Germany

    Tobias Böhmer, Norbert Pfeiffer & Adrian Gericke

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  1. Tobias Böhmer
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  2. Norbert Pfeiffer
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  3. Adrian Gericke
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Correspondence to Tobias Böhmer.

Additional information

The results presented in this article are part of the doctoral thesis of Tobias Böhmer. Supported by a grant from the Deutsche Ophthalmologische Gesellschaft (DOG)

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Böhmer, T., Pfeiffer, N. & Gericke, A. Three commercial antibodies against α1-adrenergic receptor subtypes lack specificity in paraffin-embedded sections of murine tissues. Naunyn-Schmiedeberg's Arch Pharmacol 387, 703–706 (2014). https://doi.org/10.1007/s00210-014-0992-2

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  • DOI: https://doi.org/10.1007/s00210-014-0992-2

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