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Activation of β-adrenoceptors mimics preconditioning of rat-isolated atria and ventricles against ischaemic contractile dysfunction

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Abstract

The effects of ischaemia and reoxygenation on cardiac contractile function can be abrogated by ischaemic preconditioning (IPC). We tested whether β-adrenoceptor agonists could mimic IPC and whether IPC was dependent on β-adrenoceptor activation in rat-isolated cardiac tissues. Paced left atria and right ventricular strips were set-up in Krebs solution and isometric developed tension recorded. Ischaemia was simulated by replacing with hypoxic glucose-free Krebs solution for 30 min. IPC and isoprenaline (10−7 M) preconditioning for 10 min were examined. Developed tension post-reoxygenation was expressed as a percentage of the pre-ischaemic baseline. Recovery at 15 min was significantly increased by IPC in atria (47 ± 4.0% vs. 29.3 ± 1.7%, p < 0.05) and ventricles (39.0 ± 5.2% vs. 22.4 ± 2.8%, p < 0.05). At 60 min, isoprenaline-treated atria recovery (75.8 ± 16.6%) was significantly (p < 0.05) greater than controls (47.9 ± 2.3%). Propranolol (10−6 M) abolished both effects. Therefore, both IPC and β-adrenoceptor agonist-induced improvement of contractile recovery was propranolol-sensitive and β-adrenoceptor-mediated.

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Abbreviations

βP:

β-adrenoceptor-mediated preconditioning

IPC:

ischaemic preconditioning

Pro:

propranolol

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Acknowledgement

This work was funded by a British Heart Foundation Studentship (FS-05-075-19397) awarded to P.E.P. for which the authors are grateful.

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Authors and Affiliations

  1. Pharmacology Division, Welsh School of Pharmacy, Cardiff University, King Edward VII Avenue, Cathays Park, Cardiff, CF10 3NB, UK

    Peter E. Penson, William R. Ford, Emma J. Kidd & Kenneth J. Broadley

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  1. Peter E. Penson
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  2. William R. Ford
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  3. Emma J. Kidd
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  4. Kenneth J. Broadley
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Correspondence to Kenneth J. Broadley.

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Penson, P.E., Ford, W.R., Kidd, E.J. et al. Activation of β-adrenoceptors mimics preconditioning of rat-isolated atria and ventricles against ischaemic contractile dysfunction. Naunyn-Schmied Arch Pharmacol 378, 589–597 (2008). https://doi.org/10.1007/s00210-008-0331-6

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  • DOI: https://doi.org/10.1007/s00210-008-0331-6

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