Abstract
Wood dust exposure has been found to be an occupational hazard, being linked to an enhanced incidence of various neoplasias. Here we performed an experiment to evaluate the ability of solvent extracts of natural woods to induce chromosome aberrations in respiratory cells in culture. Human embryonic lung cells, MRC-5, grown in Dolbecco’s medium were exposed to various concentrations of the dust extracts of pesticide-free (untreated) beech, oak and pine woods. Three concentrations per extract with and without metabolic activation (S9) and 100 metaphase cells per dose were examined for possible structural aberrations. Although no dose-dependent activity could be found with any extract in the presence of S9, most aberrations observed were of the chromatid type caused by oak wood. Dose-dependent chromosomal breaks caused by oak and chromatid breaks caused by both beech and oak were observed in the absence of S9. These data might support the early hypothesis that hard wood dust per se contains some in vivo genotoxic and thus possibly carcinogenic components.
References
Auerbach AD, Adler B, Changanti RS (1981) Prenatal and postnatal diagnosis and cancer detection of Fancoli anemia by a cytogenetic method. Pediatrics 67: 128–135
Dunnett CW (1955) A multiple comparison procedure for comparing several treatments with a control. J Am Statist Assoc 50: 1096–1121
Jacobs JP, Jones CM, Baille JP (1970) Characteristics of a human diploid cell designated MRC-5. Nature 227: 168–170
Macbeth R (1956) Malignant disease of the paranasal sinuses. J Otolaryngol 79: 592–612
Maron DM, Ames BN (1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173–215
McGregor DB (1982) Mutagenicity of wood dust. In: The carcinogenicity and mutagenicity of wooddust. Scientific Report No. 1, MRC Environmental Unit, Southampton, pp 26–29
Mohtashamipur E, Norpoth K (1989) Nuclear aberrations in the small intestine of mice and bacterial mutagenicity caused by a fraction isolated from beech wood dusts. Proc Am Assoc Cancer Res 30: 139
Mohtashamipur E, Norpoth K, Hallerberg B (1986) A fraction of beech wood mutagenic in the Salmonella/mammalian microsome assay. Int Arch Occup Environ Health 58: 227–234
Mohtashamipur E, Norpoth K, Ernst H, Mohr U (1989a) The mouse-skin carcinogenicity of a mutagenic fraction from beech wood dusts. Carcinogenesis 10: 483–487
Mohtashamipur E, Norpoth K, Lühmann F (1989b) Cancer epidemiology of woodworking. J Cancer Res Clin Oncol 115: 503–515
Nelson E, Zhou Z, Carmichael PL, Norpoth K, Fu J (1993) Genotoxic effects of subacute treatments with wood dust extracts on the nasal epithelium of rats: assessment by the micronucleus and 32P-postlabelling. Arch Toxicol 67: 586–589
Nylander LA, Dement JM (1993) Carcinogenic effects of wood dust: review and discussion. Am J Ind Med 24: 619–647
Savage JRK (1976) Classification and relationships of induced chromosomal structure changes. J Med Genet 13: 103–122.
Schmezer P, Kuchenmeister F, Klein RG, Pool-Zobel BL, Fengel D, Stehlin J, Wolf J (1994) Untersuchung zum gentoxischen Potential verschiedener Holzextrakte und ausgewählter Zusatzstoffe aus der Holzwirtschaft. Arbeitsmed Sozialmed Umweltmed, Sonderhelft 21: 13–17
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Zhou, Z.C., Norpoth, K.H. & Nelson, E. Genotoxicity of wood dust in a human embryonic lung cell line. Arch Toxicol 70, 57–60 (1995). https://doi.org/10.1007/s002040050249
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DOI: https://doi.org/10.1007/s002040050249