Abstract
In a preceding study, we reported that the numbers of macrophages and polymorphonuclear leukocytes (PMN) were increased in bronchoalveolar lavage fluid (BALF) following the intratracheal instillation of nickel sulfate (NiSO4) in rats. In the present study, BALF chemotactic activities for both macrophages and PMN were measured to investigate if the increases of these inflammatory cells in BALF depend on increases in chemotactic activities in epithelial lining fluid (ELF) of the lung. Both the number of PMN and the PMN chemotactic activity peaked at 2 days post-instillation and they were significantly correlated. However, the PMN chemotactic activity was inversely correlated with concentration of leukotriene B4 (LTB4), a well-known chemotaxin. Although PMN were not observed in control BALF, LTB4 concentration in the control ELF (ca. 5×10−7 M) was estimated to have a potential to attract PMN chemotactically through a membrane in in vitro migration assay. These results suggest that the presence of LTB4 in ELF itself does not trigger transpulmonary PMN infiltration. The rat BALF were fractionated by high performance liquid chromatography (HPLC), and PMN chemotactic activity of each fraction was measured. The elution profiles of PMN chemotactic activity showed that there were at least two different chemotaxins in BALF obtained from the NiSO4-exposed rats. Macrophage chemotactic activity in BALF also peaked at 2 days post-instillation. However, the number of macrophages was not significantly correlated with the chemotactic activity for macrophage in BALF. The HPLC study showed that the macrophage chemotactic substance in the BALF obtained from NiSO4-exposed rats was different from complement fragment (C5a) and its MW was estimated to be 10–12 kD.
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Baggiolini M, Dewald B (1985) The neutrophil. Int Arch Allergy Appl Immunol (Suppl 1) 76: 13–20
Baird BR, Cheronis JC, Sandhaus RA, Berger EM, White CW, Repine JE (1986) O2 metabolites and neutrophil elastase synergistically cause edematous injury in isolated rat lungs. J Appl Physiol 61: 2224–2229
Blussé A, Alblas VO, Furth RV (1979) Origin, kinetics, and characteristics of pulmonary macrophages in the normal steady state. J Exp Med 149: 1504–1518
Böyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Invest 21: 77–89
Broaddus VC, Hebert CA, Vitangcol RV, Hoeffel JM, Bernstein MS, Boylan AM (1992) Interleukin-8 is a major neutrophil chemotactic factor in pleural liquid of patients with empyema. Am Rev Respir Dis 146: 825–830
Doerschuk CM (1992) The role of CD18-mediated adhesion in neutrophil sequestration induced by infusion of activated plasma in rabbits. Am J Respir Cell Mol Biol 7: 140–148
Downey GP, Worthen GS, Henson PM, Hyde DM (1993) Neutrophil sequestration and migration in localized pulmonary inflammation. Am Rev Respir Dis 147: 168–176
Furfaro S, Berman JS (1992) The relation between cell migration and activation in inflammation: Beyond adherence. Am J Respir Cell Mol Biol 7: 248–250
Gie RP, Doerschuk CM, English D, Coxson HO, Hogg JC (1991) Neutrophil-associated lung injury after the infusion of activated plasma. J Appl Physiol 70: 2471–2478
Hatch GE (1992) Comparative biochemistry of airway lining fluid. In: Parent RA (ed) Comparative biology of the normal lung. CRC Press, Boca Raton, Florida, pp 617–632
Hatch GE, Gardner DE, Menzel DB (1980) Stimulation of oxidant production in alveolar macrophages by pollutant and latex particles. Environ Res 23: 121–136
Hirano S, Shimada T, Osugi J, Kodama N, Suzuki KT (1994) Pulmonary clearance and inflammatory potency of intratracheally instilled and acutely inhaled nickel sulfats in rats. Arch Toxicol 68: (in press)
Inano H, English D, Doerschuk CM (1992) Effect of zymosan-activated plasma on the deformability of rabbit polymorphonuclear leukocytes. J Appl Physiol 73: 1370–1376
Kindt GC, Gadek JE, Weiland JE (1991) Initial recruitment of neutrophils to alveolar structures in acute lung injury. J Appl Physiol 70: 1575–1585
Kleeberger SR, Hudak BB (1992) Acute ozone-induced change in airway permeability: role of infiltrating leukocytes. J Appl Physiol 72: 670–676
Kobzik L, Godleski JJ, Barry BE, Brain JD (1988) Isolation and antigenic identification of hamster lung interstitial macrophages. Am Rev Respir Dis 138: 908–914
Koyama S, Rennard SI, Daughton D, Shoji S, Robbins RA (1991) Bronchoalveolar lavage fluid obtained from smokers exhibits increased monocyte chemokinetic activity. J Appl Physiol 70: 1208–1214
Odekon LE, Frewin MB, Del Vecchio P, Saba TM, Gudewicz PW (1991) Fibronectin fragments released from phorbol ester-stimulated pulmonary artery endothelial cell monolayers promote neutrophil chemotaxis. Immunology 74: 114–120
Ozaki T, Hayashi H, Tani K, Ogushi F, Yasuoka S, Ogura T (1992) Neutrophil chemotactic factors in the respiratory tract of patients with chronic airway diseases or idiopathic pulmonary fibrosis. Am Rev Respir Dis 145: 85–91
Pardi R, Inverardi L, Bender JR (1992) Regulatory mechanisms in leukocyte adhesion: flexible receptors for sophisticated travelers. Immunol Today 13: 224–230
Parsons PE, Fowler AA, Hyers TM, Henson PM (1985) Chemotactic activity in bronchoalveolar lavage fluid from patients with adult respiratory distress syndrome. Am Rev Respir Dis 132: 490–493
Pilewski JM, Albelda SM (1993) Adhesion molecules in the lung. Am Rev Respir Dis 148 (Suppl): S31–37
Radermecker M, Bury T, Saint-Remy P (1989) Effect of histamine on chemotaxis and phagocytosis of human alveolar macrophages and blood monocytes. Int Arch Allergy Appl Immunol 88: 197–199
Riley DJ, Berg RA, Soltys RA, Kerr JS, Guss HN, Curran SF, Laskin DL (1988) Neutrophil response following intratracheal instillation of collagen peptides into rat lungs. Exp Lung Res 14: 549–563
Rodriguez R, White RR, Senior RM, Levine EA (1977) Elastase release from human alveolar macrophages: comparison between smokers and nonsmokers. Science 198: 313–314
Rosengren S, Olofsson AM, von Andrian UH, Akerlund EL, Arfors KE (1991) Leukotriene B4-induced neutrophil-mediated endothelial leakage in vitro and in vivo. J Appl Physiol 71: 1322–1330
Shak S, Goldstein IR (1984) Carbon monooxide inhibits ω-oxidation of leukotriene B4 by human polymorphonuclear leukocytes: evidence that catabolism of leukotriene B4 is mediated by a cytochrome P-450 enzyme. Biochem Biophys Res Commun 123: 475–481
Shellito J, Esparza C, Armstrong C (1987) Maintenance of the normal rat alveolar macrophage cell population. Am Rev Respir Dis 135: 78–82
Sibille Y, Reynolds HY (1990) Macrophages and polymorphonuclear neutrophils in lung defense and injury. Am Rev Respir Dis 141: 471–501
Steadman R, Irwin MH, St John PL, Blackburn WD, Heck LW, Abrahamson DR (1993) Laminin cleavage by activated human neutrophils yields proteolytic fragments with selective migratory properties. J Leuk Biol 53: 354–365
Van Furth R (1985) Cellular biology of pulmonary macrophages. Int Arch Allergy Appl Immunol 76: 21–27
Warheit DB, George G, Hill LH, Snyderman R, Brody AR (1985) Inhaled asbestos activates a complemet-dependent chemoattractant for macrophages. Lab Invest 52: 505–514
Wolpe SD, Cerami A (1989) Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J 3: 2565–2573
Worthen GS, Haslett C, Rees AJ, Gumbay RS, Henson JE, Henson PM (1987) Neurophil-mediated pulmonary vascular injury. Am Rev Respir Dis 136: 19–28
Yoshizawa Y, Nomura A, Ohdama S, Tanaka M, Morinari H, Hasegawa S (1988) The significance of complement activation in the pathogenesis of hypersensitivity pneumonitis: sequential changes of complement components and chemotactic activities in bronchoalveolar lavage fluids. Int Arch Allergy Appl Immunol 87: 417–423
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Hirano, S., Asami, T., Kodama, N. et al. Correlation between inflammatory cellular responses and chemotactic activity in bronchoalveolar lavage fluid following intratracheal instillation of nickel sulfate in rats. Arch Toxicol 68, 444–449 (1994). https://doi.org/10.1007/s002040050095
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DOI: https://doi.org/10.1007/s002040050095