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Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs

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Animal Models of Allergic Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2223))

Abstract

Bronchoalveolar lavage (BAL) is a technique used to collect the contents of the airways. The fluid recovered, called BAL fluid (BALF), serves as a dynamic tool to identify various disease pathologies ranging from asthma to infectious diseases to cancer in the lungs. A wide array of tests can be performed with BALF, including total and differential leukocyte counts (DLC), enzyme-linked immunosorbent assays (ELISA) or flow-cytometric quantitation of inflammatory mediators, such as cytokines, chemokines and adhesion molecules, and assessment of nitrate and nitrite content for estimation of nitric oxide synthase (NOS) activity. Here, we describe a detailed procedure for the collection of BALF for a variety of downstream usages, including DLC by cytological and flow-cytometry-based methods, multiplex cytokine analysis by flow cytometry, and NOS activity analysis by determining nitrate and nitrite levels.

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Acknowledgments

This work was supported by NIH grants R01-HL123494, R01-HL123494-02S1, R01-HL146705 (Venkatachalem).

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Health Professions, School of Pharmacy, North Dakota State University, Fargo, ND, USA

    Rama Satyanarayana Raju Kalidhindi, Nilesh Sudhakar Ambhore & Venkatachalem Sathish

Authors
  1. Rama Satyanarayana Raju Kalidhindi
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  2. Nilesh Sudhakar Ambhore
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  3. Venkatachalem Sathish
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Correspondence to Venkatachalem Sathish .

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Editors and Affiliations

  1. Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND, USA

    Kumi Nagamoto-Combs

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Kalidhindi, R.S.R., Ambhore, N.S., Sathish, V. (2021). Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs. In: Nagamoto-Combs, K. (eds) Animal Models of Allergic Disease. Methods in Molecular Biology, vol 2223. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1001-5_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1001-5_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1000-8

  • Online ISBN: 978-1-0716-1001-5

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