Archives of Toxicology

, Volume 86, Issue 1, pp 27–35 | Cite as

Assessment of DNA integrity (COMET assay) in sperm cells of boron-exposed workers

  • Yalçın Duydu
  • Nurşen Başaran
  • Aylin Üstündağ
  • Sevtap Aydın
  • Ülkü Ündeğer
  • Osman Yavuz Ataman
  • Kaan Aydos
  • Yalçın Düker
  • Katja Ickstadt
  • Britta Schulze Waltrup
  • Klaus Golka
  • Hermann M. Bolt
Inorganic compounds

Abstract

An extension of a male reproductive study conducted in a boric acid/borate production zone at Bandırma, Turkey, is presented. The relation between DNA-strand breaks (COMET assay, neutral and alkaline version) in sperm cells and previously described sperm quality parameters was investigated in boron-exposed males. A correlation between blood boron levels and mean DNA-strand breaks in sperm was weak, and DNA-strand breaks in sperm were statistically not different between control and exposed groups. Therefore, increasing boron exposures had no additional contribution in addition to already pre-existing DNA-strand breaks in the sperm cells. Weak but statistically significant correlations between DNA-strand breaks and motility/morphology parameters of sperm samples were observed in the neutral version of the COMET assay, while correlations between the same variables were statistically not significant in the alkaline version. A likely reason for these negative results, even in highly exposed humans, is that experimental exposures that had led to reproductive toxicity in animals were significantly higher than any boron exposures, which may be reached under realistic human conditions.

Keywords

Boric acid Borax Boron COMET assay Fertility Sperm parameters Male reproduction 

Supplementary material

204_2011_743_MOESM1_ESM.doc (29 kb)
Supplementary material 1 (DOC 29 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yalçın Duydu
    • 1
  • Nurşen Başaran
    • 2
  • Aylin Üstündağ
    • 1
  • Sevtap Aydın
    • 2
  • Ülkü Ündeğer
    • 2
  • Osman Yavuz Ataman
    • 3
  • Kaan Aydos
    • 4
  • Yalçın Düker
    • 5
  • Katja Ickstadt
    • 6
  • Britta Schulze Waltrup
    • 6
  • Klaus Golka
    • 7
  • Hermann M. Bolt
    • 7
  1. 1.Department of Toxicology, Faculty of PharmacyAnkara UniversityAnkaraTurkey
  2. 2.Department of Toxicology, Faculty of PharmacyHacettepe UniversityAnkaraTurkey
  3. 3.Department of ChemistryMiddle East Technical UniversityAnkaraTurkey
  4. 4.Department of Urology, Faculty of MedicineAnkara UniversityAnkaraTurkey
  5. 5.Bandırma State HospitalBandırmaTurkey
  6. 6.Faculty of StatisticsTU DortmundDortmundGermany
  7. 7.Leibniz Research Centre for Working Environment and Human Factors (IfADo)DortmundGermany

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