Abstract
Genetic polymorphisms in the coding exons of the CYP1A1 gene were analyzed in 100 Koreans. Three types of CYP1A1 polymorphisms, specifically G134A, G184C and A2455G, were identified with allelic frequencies of 18, 3, and 16%, respectively, and no linkage was observed among them. The novel G184C polymorphism identified in this study was associated with the mutation of an alanine residue at position 62 to proline. Other earlier-reported polymorphisms in the coding region of CYP1A1 were not detected.
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Abbreviations
- CYP :
-
Cytochrome P450
- PCR :
-
Polymerase chain reaction
References
Cascorbi I, Brockmoller J, Roots I (1996) A C4887A polymorphism in exon 7 of human CYP1A1: population frequency, mutation linkages, and impact on lung cancer susceptibility. Cancer Res 56:4965–4969
Chevalier D, Allorge D, Lo-Guidice JM, Cauffiez C, Lhermitte M, Lafitte JJ, Broly F (2001) Detection of known and two novel (M331I and R464S) missense mutations in the human CYP1A1 gene in a French Caucasian population. Hum Mutat 17:355
Crofts F, Cosma GN, Currie D, Taioli E, Toniolo P, Garte SJ (1993) A novel CYP1A1 gene polymorphism in African-Americans. Carcinogenesis 14:1729–1731
Garte S (1998) The role of ethnicity in cancer susceptibility gene polymorphisms: the example of CYP1A1. Carcinogenesis 19:1329–1332
Garte S, Gaspari L, Alexandrie AK, Ambrosone C, Autrup H, et al (2001) Metabolic gene polymorphism frequencies in control populations. Cancer Epidemiol Biomarkers Prev 10:1239–1248
Gonzalez FJ, Gelboin HV (1994) Role of human cytochromes P450 in the metabolic activation of chemical carcinogens and toxins. Drug Metab Rev 26:165–183
Hahn M, Hagedorn G, Kuhlisch E, Schackert HK, Eckelt U (2002) Genetic polymorphisms of drug-metabolizing enzymes and susceptibility to oral cavity cancer. Oral Oncol 38:486–490
Hayashi S, Watanabe J, Nakachi K, Kawajiri K (1991) Genetic linkage of lung cancer-associated MspI polymorphisms with amino acid replacement in the heme binding region of the human cytochrome P450IA1 gene. J Biochem (Tokyo) 110:407–411
Hong YS, Chang JH, Kwon OJ, Ham YA, Choi JH (1998) Polymorphism of the CYP1A1 and glutathione-S-transferase genes in Korean lung cancer patients. Exp Mol Med 30:192–198
Ingelman-Sundberg M (2001) Genetic variability in susceptibility and response to toxicants. Toxicol Lett 120:259–268
Kang D (2003) Genetic polymorphisms and cancer susceptibility of breast cancer in Korean women. J Biochem Mol Biol 36:28–34
Kim KS, Ryu SW, Kim YJ, Kim E (1999) Polymorphism analysis of the CYP1A1 locus in Koreans: presence of the solitary m2 allele. Mol Cells 9:78–83
Nan HM, Kim H, Lim HS, Choi JK, Kawamoto, T, Kang JW, Lee CH, Kim YD, Kwon EH (2001) Effects of occupation, lifestyle and genetic polymorphisms of CYP1A1, CYP2E1, GSTM1 and GSTT1 on urinary 1-hydroxypyrene and 2-naphthol concentrations. Carcinogenesis 22:787–793
Nelson DR, Koymans L, Kamataki T, Stegeman JJ, Feyereisen R, Waxman DJ, Waterman MR, Gotoh O, Coon MJ, Estabrook RW, Gunsalus IC, Nebert DW (1996) P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6:1–42
Saito S, Iida A, Sekine A, Ogawa C, Kawauchi S, Higuchi S, Ohno M, Nakamura Y (2002) 906 variations among 27 genes encoding cytochrome P450 (CYP) enzymes and aldehyde dehydrogenases (ALDHs) in the Japanese population. J Hum Genet 47:419–444
Sato M, Sato T, Izumo T, Amagasa T (2000) Genetically high susceptibility to oral squamous cell carcinoma in terms of combined genotyping of CYP1A1 and GSTM1 genes. Oral Oncol 36:267–271
Smart J, Daly AK (2000) Variation in induced CYP1A1 levels: relationship to CYP1A1, Ah receptor and GSTM1 polymorphisms. Pharmacogenetics 10:11–24
Spurr NK, Gough AC, Stevenson K, Wolf CR (1987) Msp-1 polymorphism detected with a cDNA probe for the P-450 I family on chromosome 15. Nucleic Acids Res 15:5901
Yang M, Jang JY, Kim S, Lee SM, Chang SS, Cheong HK, Lee E, Kang D, Kim H, Kawamoto T, Shin HD (2003) Genetic effects on urinary 1-hydroxypyrene levels in a Korean population. Carcinogenesis 24:1085–1089
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This study was supported, in part, by the National Institute of Toxicological Research, Seoul, Korea. Our experiments complied with the currents laws of Korea, where they were conducted.
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Park, D.W., Jin, B., Jang, D. et al. Genetic polymorphisms of CYP1A1 in a Korean population. Arch Toxicol 78, 306–308 (2004). https://doi.org/10.1007/s00204-004-0552-5
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DOI: https://doi.org/10.1007/s00204-004-0552-5