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Glucosylglycerol and proline reverse the effects of glucose on Rhodosporidium toruloides lifespan

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Abstract

Rhodosporidium toruloides is a novel cell factory used to synthesis carotenoids, biosurfactants, and biofuel feedstocks. However, research on R. toruloides has generally centred on the manufacture of biochemicals, while analyses of its longevity have received scant attention. Understanding of R. toruloides longevity under different nutrient conditions could help to improve its biotechnological significance and metabolite production. Glucosylglycerol (GG) and proline are osmoprotectants that could revert the harmful effects of environmental stress. This study examined how GG and proline affect R. toruloides strain longevity under glucose nutrimental stress. Herein, we provide evidence that GG and proline enhance cell performance and viability. These compatible solutes neutralises the pro-ageing effects of high glucose (10% glucose) on the yeast cell and reverse its cellular stress. GG exhibits the greatest impact on lifespan extension at 100 mM, whereas proline exerts effect at 2 mM. Our data reveal that these compounds significantly affect the culture medium osmolarity. Moreso, GG and proline decreased ROS production and mitohormetic lifespan regulation, respectively. The data indicates that these solutes (proline and GG) support the longevity of R. toruloides at a pro-ageing high glucose culture condition.

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Datasets available from the corresponding author upon reasonable request.

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Acknowledgements

CKO wishes to thank CAS-TWAS President’s Fellowship for the award of PhD scholarship (2019A8003833001).

Funding

We received no funding for conducting this study.

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Authors and Affiliations

  1. Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Rd, Dalian, 116023, China

    Chuks Kenneth Odoh & Rasool Kamal

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Chuks Kenneth Odoh

  3. Tecnológico Nacional de México/ITS de Ciudad Hidalgo, Ciudad Hidalgo, Michoacán, Mexico

    Luis Alberto Madrigal-Perez

  4. Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Rd, Dalian, 116023, China

    Rasool Kamal

Authors
  1. Chuks Kenneth Odoh
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  2. Luis Alberto Madrigal-Perez
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  3. Rasool Kamal
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Contributions

CKO: conceptualisation, methodology, investigation, data curation, writing-original draft, validation, reviewing, revision, and editing. LAMP: writing original draft, review, editing RK: reviewing, editing, and suggestion.

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Correspondence to Chuks Kenneth Odoh.

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Communicated by Yusuf Akhter.

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Odoh, C.K., Madrigal-Perez, L.A. & Kamal, R. Glucosylglycerol and proline reverse the effects of glucose on Rhodosporidium toruloides lifespan. Arch Microbiol 206, 195 (2024). https://doi.org/10.1007/s00203-024-03930-8

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  • DOI: https://doi.org/10.1007/s00203-024-03930-8

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