Abstract
Brazilian biomes are important sources for environmental microorganisms, including efficient metabolic machineries, like actinomycetes. These bacteria are known for their abilities to produce many bioactive compounds, including enzymes with multiple industrial applications. The present work aimed to evaluate lignocellulolytic abilities of actinomycetes isolated from soil and rhizosphere samples collected at Caatinga, Atlantic and Amazon Forest. Laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and cellulase were evaluated for their efficiency. These enzymes have an essential role in lignin decomposition, through oxidation of phenolic and non-phenolic compounds, as well as enzymatic hydrolysis of vegetal biomass. In this sense, a total of 173 actinomycetes were investigated. Eleven (11) of them were selected by their enzymatic performance. The actinomycete AC166 displayed some activity in all analysed scenarios in terms of Lac, MnP and LiP activity, while AC171 was selected as the most promising strain, showing the following activities: 29.7 U.L−1 for Lac; 2.5 U.L−1 for LiP and 23 U.L−1 for MnP. Cellulolytic activities were evaluated at two pH conditions, 4.8 and 7.4, obtaining the following results: 25 U.L−1 and 71 U.L−1, respectively. Thermostability (4, 30 and 60 o C) and salinity concentrations (0 to 4 M) and pH variation (2.0 to 9.0) stabilities of the obtained LiP and Lac enzymatic extracts were also verified. The actinomycete strain AC171 displayed an adaptable response in distinct pH and salt profiles, indicating that bacterial LiP was some halophilic type. Additionally, the strain AC149 produced an alkali and extreme halophilic lignin peroxidase, which are promising profiles for their future application under lignocellulosic biomass at bioethanol biorefineries.
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The data that support the findings of this study are available on request from the corresponding author.
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Acknowledgements
This study was supported by the Brazilian research agencies FAPESP (Process 2016/23685-7), CAPES (finance code 001) and CNPq (scholarships). The authors would like to register their gratitude to Brazilian Agricultural Research Corporation, EMBRAPA Environment for providing the samples.
Funding
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior,001,001,001,001,001,001,Conselho Nacional de Desenvolvimento Científico e Tecnológico,Fundação de Amparo à Pesquisa do Estado de São Paulo,Brasil,2016/23685-7
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Suzan Pantaroto de Vasconcellos and Debora Noma Okamoto contributed equally to this manuscript. Conceptualization: Debora Noma Okamoto and Suzan Pantaroto de Vasconcellos; Formal analysis: Guilherme Mascalchi de Figueiredo, Mariana Rocha Roswell and Itamar Soares de Melo; Investigation: Kelly Matos Marques, Fernanda Camila Gutierres, Guilherme Gonçalves Guerini, Guilherme Mascalchi de Figueiredo, Vitor Gonçalves Vital, Mariana Rocha Roswell and Itamar Soares de Melo; Methodology: Vitor Baptista Ferrari, Lidiane Maria dos Santos Lima and Marghuel Aparecida Vieira Silveira; Supervision: Suzan Pantaroto de Vasconcellos; Visualisation: Debora Noma Okamoto; Writing – original draft, Vitor Baptista Ferrari, Lidiane Maria dos Santos Lima, Marghuel Aparecida Vieira Silveira, Debora Noma Okamoto and Suzan Pantaroto de Vasconcellos.
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Ferrari, V.B., dos Santos Lima, L.M., de Matos Marques, K. et al. Caatinga, Amazon and Atlantic Forest as natural sources for microbial lignocellulolytic enzymes. Arch Microbiol 206, 161 (2024). https://doi.org/10.1007/s00203-024-03883-y
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DOI: https://doi.org/10.1007/s00203-024-03883-y