Abstract
A novel motile bacterium was isolated from a sediment sample collected in Kochi backwaters, Kerala, India. This bacterium is Gram negative, rod shaped, 1.0–1.5 µm wide, and 2.0–3.0 µm long. It was designated as strain AK27T. Colonies were grown on marine agar displayed circular, off-white, shiny, moist, translucent, flat, margin entire, 1–2 mm in diameter. The major fatty acids identified in this strain were C18:1 ω7c, C16:0, and summed in feature 3. The composition of polar lipids in the strain AK27T included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified amino lipid, two unidentified aminophospholipids, two unidentified phospholipids, and six unidentified lipids. The genomic DNA of strain AK27T exhibited a G+C content of 56.4 mol%. Based on the analysis of 16S rRNA gene sequence, strain AK27T showed sequence similarity to M. ramblicola D7T and M. zhoushanense WM3T as 98.99% and 98.58%, respectively. Compared to other type strains of the Marinobacterium genus, strain AK27T exhibited sequence similarities ranging from 91.7% to 96.4%. When compared to Marinobacterium zhoushanense WM3T and Marinobacterium ramblicola D7T, strain AK27T exhibited average nucleotide identity values of 80.25% and 79.97%, and dDDH values of 22.9% and 22.6%, respectively. The genome size of the strain AK27T was 4.55 Mb, with 4,229 coding sequences. Based on the observed phenotypic and chemotaxonomic features, and the results of phylogenetic and phylogenomic analysis, this study proposes the classification of strain AK27T as a novel species within the genus Marinobacterium. The proposed name for this novel species is Marinobacterium lacunae sp. nov.
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Data availability
The 16S rRNA gene and genome sequences were submitted to the NCBI and available to the scientific community. The type strain is deposited in culture collection centers and is available to the scientific community.
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Acknowledgements
The authors thank the NIO and the IMTech Directors. We thank the CSIR and the DBT, Govt. India, for financial support. TNRS is grateful for projects OLP 2011 and GAP 3195 for providing facilities and financial support, respectively. We express thanks to Professor Aharon Oren for his specialist suggestions about the accurate Latin etymology and species epithet. GS express gratitude to CSIR for the fellowship. The NIO communication no. is 7100.
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The financial support for the work is from the OLP 2011 (Institutional project) and the project GAP 3195 (Ministry of Earth Sciences).
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Srinivas T. N. R., Anil Kumar P.: Conceptualization, planned study, examined data, methodology, script—original draft; Swarnaprava Behera, Aditya Singh, Sudharani P, Bhumika Vaidya, Gunjan Sharma, Sampath Kumar, G., Naveen Sagar, G.: Performed research, prepared figures.
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Communicated by Wen-Jun Li.
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The GenBank/DDBJ/EMBL accession number for the 16S rRNA gene sequence of strain AK27T is HE985072, and the whole-genome sequence is JMQN01000000.
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Tanuku, S.N.R., Pinnaka, A.K., Behera, S. et al. Marinobacterium lacunae sp. nov. isolated from estuarine sediment. Arch Microbiol 205, 294 (2023). https://doi.org/10.1007/s00203-023-03627-4
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DOI: https://doi.org/10.1007/s00203-023-03627-4