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Neiella litorisoli sp. nov., an alginate lyase: producing bacterium from South China Sea, and proposal of Echinimonadaceae fam. nov. in the order Alteromonadales

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Abstract

A Gram-stain-negative, rod-shaped bacterium, designated HB171785T, was isolated from soil sample collected from Qishui Bay, Hainan, China. The strain grew optimally at pH 7–8, 37–40 °C and with NaCl 3–4%. The predominant isoprenoid quinone was found to be Q-8 and the major fatty acids were C16:0, C16:1 ω7c/C16:1 ω6c, C18:1 ω7c/C18:1 ω6c and C12:0 3OH. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The size of the draft genome was 4.32 Mbp with G + C content 49.7%. Phylogenetic analysis of 16S rRNA gene sequence indicated that the closest phylogenetically related species were Neiella marina j221T, “Neiella holothuriorum” 126 and Echinimonas agarilytica KMM 6351T with the similarities of 98.2, 96.0 and 95.0%, respectively. The phylogenetic tree based on 16S rRNA gene and phylogenomic tree based on core genome showed that strain HB171785T clustered together with N. marina j221T, with the highest values of average nucleotide identity (82.9%) and digital DNA-DNA hybridization (25.4%). The combined phylogenetic relatedness, phenotypic and genotypic features supported the conclusion that strain HB171785T represents a novel species of the genus Neiella, for which the name Neiella litorisoli sp. nov. is proposed. The type strain is HB171785T (= MCCC 1K04625T = KCTC 82319T). In addition, Echinimonadaceae fam. nov. in the order Alteromonadales was proposed.

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Data availability

The GenBank/EMBL/DDBJ accession numbers for 16S rRNA gene and draft genome sequences of strain HB171785T are MN911325 and JACXAF000000000, respectively.

Abbreviations

AAI:

Average amino acid identity

AL:

Aminolipid

ANI:

Average nucleotide identity

APL:

Aminophospholipid

dDDH:

Digital DNA–DNA hybridization

DPG:

Diphosphatidylglycerol

L:

Lipid

LPSN:

List of prokaryotic names with standing in nomenclature

MA:

Marine agar 2216

MB:

Marine broth 2216

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PL:

Polysaccharide lyase

Q-8:

Ubiquinone 8

TLC:

Thin-layer chromatography

UBCG:

Up-to-date bacterial core gene

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Acknowledgements

We are grateful to Dr. Qiliang Lai (Third Institute of Oceanography, MNR, China) for his help in the proposal of the novel family, and generation of the bacterial core genome tree using UBCG tool.

Funding

This research was supported by grants from the Key Research and Development Project of Hainan Province (ZDYF2020182), the Financial Fund of the Ministry of Agriculture and Rural Affairs of China (NFZX2021, NHYYSWZZZYKZX2020) and Central Public-interest Scientific Institution Basal Research Fund from Chinese Government (1630052019014).

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Authors and Affiliations

  1. College of Marine Science, Hainan University, Haikou, 570228, China

    Jing-Ran Sun & Zhi-Yuan Liu

  2. Institute of Tropical Bioscience and Biotechnology, Hainan Institute for Tropical Agricultural Resources, CATAS, Haikou, 571101, China

    Jing-Ran Sun, Kun-Lian Mo, Xue Li, Yong-Hua Hu & Hui-Qin Huang

  3. Zhanjiang Experimental Station, CATAS, Zhanjiang, 524013, China

    Kun-Lian Mo, Yong-Hua Hu & Hui-Qin Huang

  4. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163000, China

    Xue Li

  5. Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Yong-Hua Hu

  6. Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bioresources, Haikou, 571101, China

    Jing-Ran Sun, Kun-Lian Mo, Yong-Hua Hu & Hui-Qin Huang

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Contributions

J-RS and XL conducted the experiments. K-LM and Y-HH conceived and designed research. Z-YL analyzed data. H-QH and J-RS wrote the manuscript and edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zhi-Yuan Liu or Hui-Qin Huang.

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Sun, JR., Mo, KL., Li, X. et al. Neiella litorisoli sp. nov., an alginate lyase: producing bacterium from South China Sea, and proposal of Echinimonadaceae fam. nov. in the order Alteromonadales. Arch Microbiol 205, 227 (2023). https://doi.org/10.1007/s00203-023-03573-1

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