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Characterization and metabolomic profiling of two pigment producing fungi from infected fruits of Indian Gooseberry

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Abstract

Two pigment producing fungi, Talaromyces atroroseus and Penicillium choerospondiatis, were isolated and identified from infected fruits of Phyllanthus emblica L. based on amplification and sequencing of internal transcribed spacer region and beta-tubulin gene. This is the first occurrence report of these two fungi from fruits of P. emblica. Culture extract containing metabolites of T. atroroseus and P. choerospondiatis contained phenolics of 26.35 mg and 30.89 mg GAE/g dry extract respectively; whereas no significant amount of flavonoids and tannins were detected. P. choerospondiatis metabolites extract showed higher DPPH and ABTS activity with IC50 values of 21.94 mg/ml and 27.03 mg/ml respectively than T. atroroseus. LC-HRMS analysis of metabolites extract of T. atroroseus revealed presence of trimethyl-isopropyl-butanamide, perlolyrine, N-hexadecanoylpyrrolidine etc. whereas P. choerospondiatis displayed presence of tangeraxanthin, ugaxanthone, daphniphylline, etc. Therefore, fungal metabolites are rich natural sources of diversified compounds that can be utilized in dyeing industries, cosmetics and novel drug development.

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Acknowledgements

We are privileged to convey our sincere gratitude to our respected Director, Prof. Sanghamitra Bandhopadhyay for providing laboratory facilities and financial support for performing this research work. We are indebted to Prof-in-Charge, Biological Sciences Division; Head, Agricultural and Ecological Research Unit, Indian Statistical Institute, for their affectionate encouragement, valuable advice and enormous laboratory facilities.

Funding

Funding was provided by Indian Statistical Institute, Kolkata (Grant no. 5521F).

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Authors and Affiliations

  1. Agricultural and Ecological Research Unit, Indian Statistical Institute, 203, B.T. Road, Kolkata, 700108, India

    Madhurima Dutta, Anjan Hazra, Ekta Bhattacharya, Rahul Bose & Suparna Mandal Biswas

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  1. Madhurima Dutta
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  2. Anjan Hazra
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  3. Ekta Bhattacharya
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  4. Rahul Bose
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  5. Suparna Mandal Biswas
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Contributions

MD—design of the experiment, investigation, collection of test data, formal analysis, drafting the article; AH—design of the experiment, investigation, formal analysis, revision of manuscript; EB—design of the experiment, investigation, formal analysis, revision of manuscript; RB—design of the experiment, investigation, formal analysis, revision of manuscript; SMB—critical revision, funding acquisition, project administration, validation, supervision.

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Correspondence to Madhurima Dutta or Suparna Mandal Biswas.

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The authors who have engaged in this study declared that they have no conflict of interests among them regarding the publication of this paper.

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Communicated by Yusuf Akhter.

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Dutta, M., Hazra, A., Bhattacharya, E. et al. Characterization and metabolomic profiling of two pigment producing fungi from infected fruits of Indian Gooseberry. Arch Microbiol 205, 141 (2023). https://doi.org/10.1007/s00203-023-03483-2

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